光透法定量粗糙裂隙生物堵塞下的隙宽

发布时间：2018-02-08 13:23

  本文关键词： 裂隙 生物堵塞 隙宽 光透法　出处：《水文地质工程地质》2017年06期 　论文类型：期刊论文

【摘要】：基于光透法定量测量裂隙隙宽的原理及在两相流中的应用,设计了一组二维粗糙裂隙模拟装置研究微生物在裂隙地下水系统中的运移行为,首次定量研究了生物堵塞过程中隙宽及分布的变化。研究结果表明:微生物大多以离散菌簇状形态存在,未能形成明显的连续生物膜,不同空间位置微生物分布具有一定的随机性,本试验中微生物接种和营养注入方式决定了微生物集中分布在裂隙底部区域;微生物生长造成裂隙平均隙宽的下降(从1 181μm降低为1 086μm,下降8.04%),此外,基于光透法测量的不同空间位置隙宽(除2个点外)在微生物生长过程中都有所下降,两次微生物接种后点2处隙宽下降最明显(从1 275μm减少到1 081μm),下降了194μm,约为15.22%。光透法作为一种高效无损的室内监测手段,将其应用于裂隙介质的生物堵塞尤其是定量计算生物膜厚度的研究中,对裂隙中生物堵塞研究具有重要的参考意义。
[Abstract]:Based on the principle of measuring fissure gap width by photo-permeation method and its application in two-phase flow, a set of two-dimensional rough fracture simulator was designed to study the migration behavior of microorganisms in fractured groundwater system. The changes of gap width and distribution in the process of biological blockage were quantitatively studied for the first time. The results showed that most of the microorganisms existed in the form of discrete bacteria clusters and could not form obvious continuous biofilms. The distribution of microorganism in different space is random. In this experiment, the way of inoculation and nutrition injection determines the distribution of microorganism in the bottom of fissure. The average gap width (from 1 181 渭 m to 1 086 渭 m) was decreased by 8. 04 渭 m. In addition, the gap width at different locations (except 2 points) measured by optical transmission method decreased during the growth process of microorganism, and the mean gap width decreased from 1 181 渭 m to 1 086 渭 m, and decreased by 8. 04 渭 m from 1 181 渭 m to 1 086 渭 m. The gap width at the two points after inoculation was the most obvious (from 1 275 渭 m to 1 081 渭 m, 194 渭 m, about 15.22). Its application in the study of biological blockage in fractured media, especially in the quantitative calculation of biofilm thickness, has an important reference significance for the study of biofilm in fissures.
【作者单位】： 南京大学地球科学与工程学院/表生地球化学教育部重点实验室;东华理工大学水资源与环境工程学院;
【基金】：国家自然科学基金项目(41472212,41602258)
【分类号】：X523
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