陕北石油污染区土壤细菌群落结构解析

发布时间：2018-02-12 13:52

本文关键词： 石油污染微生物群落结构微生物多样性　出处：《环境污染与防治》2017年11期 　论文类型：期刊论文

【摘要】：为了解陕北地区石油污染对土壤微生物群落结构的影响,采用高通量测序对石油污染土壤中细菌群落结构进行研究,考察了石油污染土样的理化性质、可培养微生物数量及细菌群落结构,探讨石油污染程度与细菌群落结构间的关系。结果表明,石油污染土样中残油量为28.7~870.0mg/kg,相对于未污染土样,石油污染土样的总碳随残油量的增加呈增加趋势。石油污染土样中可培养细菌数量大于未污染土样,而可培养放线菌数量则相反。细菌群落结构分析表明,石油污染土样中细菌群落数量显著低于未污染土样,操作分类单元(OTU)数从未污染土样的7 707个最低下降到2 034个,且物种丰富度显著降低,其中厚壁菌门、放线菌门及变形菌门为优势菌群,随土壤残油量增加,厚壁菌门、变形菌门及拟杆菌门比例显著上升,而酸杆菌门、芽单胞菌门等比例则显著降低。
[Abstract]:In order to understand the effect of petroleum pollution on soil microbial community structure, the bacterial community structure in petroleum contaminated soil was studied by high-throughput sequencing, and the physical and chemical properties of petroleum contaminated soil were investigated. The relationship between the degree of petroleum pollution and the structure of bacterial community was discussed. The results showed that the residual oil content of petroleum contaminated soil was 28.770 mg / kg, which was higher than that of uncontaminated soil. The total carbon of petroleum contaminated soil increased with the increase of residual oil content. The number of culturable bacteria in petroleum contaminated soil was larger than that in uncontaminated soil, but the number of culturable actinomycetes was opposite. The number of bacterial communities in oil-contaminated soil samples was significantly lower than that in uncontaminated soil samples, and the lowest number of 7 707 soil samples, which had never been contaminated, was reduced to 2 034, and the species richness was significantly decreased. Actinomycetes and Proteus were the dominant flora. With the increase of residual oil in soil, the proportion of phylum, Proteus and Bacteroides increased significantly, while the proportion of acidobacterium and phylomonas decreased significantly.
【作者单位】：陕西省微生物研究所;
【基金】：西部之光人才培养项目(No.K2012-02) 陕西省科学技术研究发展计划项目(No.2011K08-31) 陕西省科学院人才培养专项(No.2014k-27)
【分类号】：X172;X53

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