水稻秸秆浸泡液对蓝藻和绿藻生长选择性抑制作用

发布时间：2018-02-25 04:27

  本文关键词： 化感作用 流式细胞仪 水稻秸秆 铜绿微囊藻 抑制作用　出处：《环境科学》2017年07期 　论文类型：期刊论文

【摘要】：运用流式细胞仪研究了水稻秸秆浸泡液(RSE)对藻类生长、形态学特征(细胞大小)和生理参数(叶绿素荧光强度)的影响.RSE对有毒、无毒铜绿微囊藻(Microcystis aeruginosa)、有毒水华鱼腥藻(Anabaena flos-aquae)、鱼害微囊藻(Microcystis ichthyoblabe)这4种蓝藻和羊角月牙藻(Selenastrum capricornutum)、蛋白核小球藻(Chlorella pyrenoidosa)、斜生栅藻(Scenedesmus obliqnus)这3种常见绿藻生长的抑制作用具有选择性.RSE浓度为2.0~10.0 g·L~(-1)时,可以显著抑制蓝藻的生长,同时强烈影响了藻细胞的叶绿素荧光强度和细胞大小.有毒水华鱼腥藻、鱼害微囊藻、有毒和无毒铜绿微囊藻的7d EC50分别为1.72、2.21、2.92和5.72 g·L~(-1).暴露于RSE的蛋白核小球藻和斜生栅藻,细胞生长被促进了并观测到有群体形成;而对羊角月牙藻来说,当RSE浓度为1.0~4.0 g·L~(-1)时月牙藻生长和叶绿素荧光强度被抑制,但是当RSE浓度为8.0~10.0g·L~(-1)时,月牙藻生长被显著促进.总之,RSE对蓝藻生长的抑制效果强于羊角月牙藻、蛋白核小球藻和斜生栅藻,蓝藻光合系统Ⅱ反应中心的敏感性和绿藻群体的形成可能是造成两者对水稻秸秆敏感性不同的重要原因.
[Abstract]:The effects of rice straw soaking solution (RSE) on algae growth, morphological characteristics (cell size) and physiological parameters (chlorophyll fluorescence intensity) were studied by flow cytometry. These four species of cyanobacteria and Selenastrum capricornum, Chlorella pyrenoidosaanae, Anabaena flos-aquaeae, Anabaena flos-aquaeae, Microcystis ichthyoblabe4 and Selenastrum capricornutumum, Chlorella pyrenoidosaanus, Scenedesmus obliqnus, have selective inhibitory effects on the growth of these four species of cyanobacteria and Selenastrum capricornutum. When the RSE concentration was 2.0 ~ 10.0 g 路L ~ (-1), It can significantly inhibit the growth of cyanobacteria and strongly affect the chlorophyll fluorescence intensity and cell size of algal cells. The 7d EC50 of toxic and non-toxic microcystis aeruginosa were 1.72O2.21G / L 2.92 and 5.72g 路L ~ (-1) 路L ~ (-1) respectively. The cell growth of Chlorella cereus and Chlorella obliquus exposed to RSE was promoted and colony formation was observed. When the concentration of RSE was 1.0 g 路L ~ (-1), the growth and the fluorescence intensity of chlorophyll were inhibited, but when the concentration of RSE was 8.0 ~ 10.0 g 路L ~ (-1), the growth of caterpillar was significantly promoted. The sensitivity of photosynthetic system 鈪，

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