磷霉素制药废水的生物强化处理及应用研究

发布时间：2018-03-03 05:39

本文选题：磷霉素制药废水　切入点：生物强化技术　出处：《中国环境科学研究院》2017年硕士论文　论文类型：学位论文

【摘要】：磷霉素是一种广谱性抗菌素,对革兰氏阳性菌和阴性菌都具有较高的抗菌活性。磷霉素的生产过程中产生大量废水,这些废水如果不经处理排入河道将对生态环境和人类健康造成巨大威胁。磷霉素废水的现有处理方法中,生物法由于其经济性受到企业青睐。然而,常规的生物处理法中微生物受抗生素的抑制,处理效率有限。本研究采用生物强化技术对磷霉素废水进行处理,对从制药废水处理设施中分离的6种磷霉素高效降解菌进行复配,并优化了生长条件,并采取直接投加和用载体固定高效菌两种投加方式的生物强化技术进行处理,分析了废水处理过程中高效菌的数量变化。为了获得最佳的复配比,采用微生物分类上逐步扩大差异性的方法,将6支磷霉素高效降解菌N1、N2、N3、P1、P2、P3分两步进行复配。在接种比例为10%(V/V),磷霉素浓度为30.00 mg/L,培养温度为25℃、摇床转速为120r/min的条件下获得了最佳复配比例,当N1:N2:N3:P1:P2:P3的比例为1:1:1:1:1:1:1时对磷霉素的去除率最高,24 h的去除率为86.06%。投加高效菌前后,比较了 CASS反应器对磷霉素制药废水的去除效率。当进水磷霉素浓度为10.00 mg/L左右,土著污泥MLSS为3100.00 mg/L,HRT为6天时,土著污泥对磷霉素去除率低于12.20%;按土著污泥MLSS与复配磷霉素高效降解菌MLSS的比为1:1投加复配高效菌,投加后混合液的MLSS为2312.00 mg/L,进水磷霉素浓度23.60-38.00 mg/L,HRT为6天,经过10个周期的运行对磷霉素的去除率始终维持在80%以上。土著污泥高效菌N1、N2、N3的相对丰度分别为78.53 copy/ugDNA、250.66 copy/ugDNA、108.68copy/ugDNA。投加高效菌与土著污泥混合后,高效菌N1、N2、N3的相对丰度分别为 1.92 × 104 copy/ugDNA、293.78 copy/ugDNA、1.21 × 104 copy/ugDNA。稳定后 N1、N2、N3 的相对丰度分别为 123.65 copy/ugDNA、469.68 copy/ugDNA、181.19 copy/ugDNA。投加高效菌后,混合污泥中N1、N2、N3的相对丰度明显高于投加高效菌前的丰度,这可能就是其处理效果优于土著污泥的原因。为了比较不同填料对磷霉素高效降解菌的固定化效果,CASS反应器中分别投加了PE和PU-GEL两种填料。在进水COD为2500.00～4700.00 mg/L条件下,HRT为6天,填充PE填料的反应器COD去除率稳定在91.00%以上,出水COD在300.00 mg/L以下;进水有机磷浓度为34.50～48.10 mg/L,出水有机磷在4.70 mg/L以下,去除率在87.50%～91.90%之间;进水磷霉素浓度为22.70～39.70 mg/L,出水磷霉素的在1.20～3.40 mg/L,磷霉素去除率在89.90%～94.70%之间。填充PU-GEL填料的反应器的COD去除率稳定在93.10%以上,出水COD在229.00mg/L以下;进水有机磷浓度为36.70～67.70 mg/L,出水机磷在4.40mg/L以下,去除率在91.10%～95.20%之间;进水磷霉素浓度为21.90～39.20 mg/L,出水磷霉素在0.80～1.60 mg/L,磷霉素去除率稳定在95.00%左右。系统稳定后填充PE填料的反应器中高效菌N1、N2、N3的相对丰度分别为123.40～276.50 copy/ugDNA、118.80～549.70 copy/ugDNA、157.30～587.70 copy/ugDNA;填充 PU-GEL填料的反应器中高效菌N1、N2、N3的相对丰度分别为112.90～970.20 copy/ugDNA、219.10～981.70 copy/ugDNA、404.70～2035.30copy/ugDNA。PE 填料上高效菌的丰度小于PU-GEL填料,投加PU-GEL填料后系统稳定性优于投加PE填料。
[Abstract]:Fosfomycin is a broad-spectrum antibiotic, has high antibacterial activity against gram positive and negative bacteria to produce large amounts of wastewater. The production process of fosfomycin, if the wastewater discharged into the river will be treated to the ecological environment and human health caused great threat. The existing processing method of fosfomycin wastewater. Biological method because of its economy favored by enterprises. However, conventional biological treatment by antibiotics microbial inhibition, the processing efficiency is limited. This research adopts the bioaugmentation of fosfomycin wastewater, 6 kinds of fosfomycin isolated from pharmaceutical wastewater treatment facilities in the pheromone degrading bacteria were studied, and optimization of the growth conditions, and take direct feeding and use of immobilized microbial two dosing method of bioaugmentation technology for processing, analysis of the changes in the number of bacteria in the wastewater treatment process in order to receive. The best mixing ratio, using microbial classification and gradually expand the differences, the 6 branch of fosfomycin degrading bacteria N1, N2, N3, P1, P2, P3 was divided into two steps. In the inoculation ratio was 10% (V/V), fosfomycin concentration was 30 mg/L, culture temperature was 25 C, the shaking speed won the best proportion is 120r/min, when the ratio of N1:N2:N3:P1:P2:P3 to 1:1:1:1:1:1:1 of phosphonomycin the highest removal rate of 24 h, the removal rate of 86.06%. before and after the addition of high efficiency bacteria, compared the removal efficiency of CASS reactor of fosfomycin pharmaceutical wastewater. When the inflow of phosphonomycin the concentration is about 10 mg/L, indigenous sludge MLSS 3100 mg/L HRT for 6 days, a native of fosfomycin sludge removal rate of less than 12.20%; according to the native sludge MLSS and compound phosphonomycin degrading bacteria MLSS is 1:1 dosage of compound high bacteria, after adding mixed liquor MLSS is 2312 mg/L. In Water fosfomycin concentrations of 23.60-38.00 mg/L, HRT for 6 days, after 10 cycles of operation of phosphonomycin removal rate has remained above 80%. Native sludge bacteria N1, N2, the relative abundance of N3 were 78.53 copy/ugDNA, 250.66 copy/ugDNA, 108.68copy/ugDNA. dosage, sludge mixed with indigenous bacteria, high efficiency from N1, N2, N3 relative abundance were 1.92 * 104 copy/ugDNA, 293.78 copy/ugDNA, 1.21 x 104 copy/ugDNA. after N1, N2, the relative abundance of N3 were 123.65 copy/ugDNA, 469.68 copy/ugDNA, 181.19 copy/ugDNA. after adding bacteria, mixed sludge in N1, N2, the relative abundance of N3 was significantly higher than the dosage the abundance of bacteria before, this may be the cause of the effect is better than the native sludge. In order to compare the effect of fillers on the immobilization of fosfomycin degrading bacteria in CASS reactor, respectively adding PE and PU-GEL two kinds of fillers In the influent COD is 2500 ~ 4700 mg/L, HRT for 6 days, the reactor filled with COD PE material removal rate was over 91%, the effluent COD is below 300 mg/L in water; organic phosphorus concentration is 34.50 ~ 48.10 mg/L, the effluent organic phosphorus below 4.70 mg/L, the removal rate of 87.50% ~ 91.90%; the inlet of fosfomycin concentration is 22.70 ~ 39.70 mg/L, the effluent of fosfomycin in 1.20 ~ 3.40 mg/L, the removal rate of fosfomycin in 89.90% ~ 94.70%. The reactor filled with PU-GEL filler COD removal rate was over 93.10%, the effluent COD is below 229.00mg/L; the influent organic phosphorus concentration is 36.70 ~ 67.70 mg/L. Water machine p below 4.40mg/L, the removal rate of 91.10% ~ 95.20%; water fosfomycin concentration is 21.90 ~ 39.20 mg/L, the effluent of fosfomycin in 0.80 ~ 1.60 mg/L, the removal rate of phosphonomycin stable at around 95%. The stability of the system after filling filling in PE reactor Bacteria N1, N2, the relative abundance of N3 were 123.40 ~ 276.50 copy/ugDNA, copy/ugDNA 118.80 ~ 549.70, 157.30 ~ 587.70 copy/ugDNA; N1, bacteria reactor PU-GEL stuffing in N2, the relative abundance of N3 were 112.90 ~ 970.20 copy/ugDNA, 219.10 ~ 981.70 copy/ugDNA, the abundance of 404.70 ~ 2035.30copy/ugDNA.PE packing bacteria less than PU-GEL filler, adding PU-GEL filler after the system stability is better than adding PE filler.

【学位授予单位】：中国环境科学研究院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：X787

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