高效反硝化细菌的快速培养及群落结构多样性分析

发布时间：2018-03-11 02:34

本文选题：快速培养　切入点：高效反硝化细菌　出处：《环境科学》2017年09期 　论文类型：期刊论文

【摘要】：培养高效的反硝化细菌可提高污水处理效率.本实验为序批式实验,以Ⅰ号、Ⅱ号发酵液为碳源,采用梯度提高硝氮的方式,培养高效的反硝化细菌,从中选择培养更加快速的发酵液.并采用高通量测序技术分析反硝化细菌的生物群落结构和多样性的变化.结果表明,Ⅱ号发酵液能够在第11 d便实现高效反硝化细菌[300 mg·(L·h)-1]的快速培养,比Ⅰ号发酵液提前了17 d,同时,高效反硝化细菌系统对氨氮和总磷有一定的去除效果,最大去除速率分别为34.43 mg·(L·h)-1和2.98mg·(L·h)-1.高通量测序分析结果表明,污泥经过驯化培养,物种丰度和多样性降低,但发挥反硝化作用的优势菌群的类别和比例得到增大;细菌的组成及数量发生了较大的改变,最终发挥高效反硝化作用的核心菌属为Thauera和Pseudomonas.另外,反硝化聚磷菌科(Rhodocyclaceae和Pseudomonadaceae)和异养硝化菌属(Pseudomonas、Alcaligenes、Bacillus和Comamonas)的存在,验证了系统对氨氮和总磷的去除能力.
[Abstract]:The high efficiency denitrifying bacteria were cultured in the batch experiment, the fermentation broth I and II were used as carbon source, and the denitrifying bacteria were cultured by gradient increasing nitrate nitrogen. The fermentation broth was selected to grow faster. The changes of biome structure and diversity of denitrifying bacteria were analyzed by high-throughput sequencing technique. The culture of high efficiency denitrifying bacteria [300 mg 路L 路h ~ (-1)] was achieved on the 11th day, which was 17 days earlier than that of No. 1 fermentation broth. At the same time, the high efficiency denitrifying bacteria system could remove ammonia nitrogen and total phosphorus to a certain extent. The maximum removal rates were 34.43 mg 路L 路hn-1 and 2.98 mg 路L 路hn-1, respectively. The results of high-throughput sequencing showed that the species abundance and diversity decreased after the sludge was acclimated, but the type and proportion of dominant bacteria that played a role in denitrification were increased. The composition and quantity of bacteria changed greatly, and the core bacteria of high efficiency denitrification were Thauera and Pseudomonas. In addition, the presence of Rhodocyclaceae and Pseudomonadaceaeand Pseudomonas Alcaligenes Bacillus and Comonas. The removal capacity of ammonia nitrogen and total phosphorus was verified.
【作者单位】：北京工业大学建筑工程学院北京市水质科学与水环境恢复工程重点实验室;
【分类号】：X172;X703

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