XH02菌强化反应器脱氮过程中菌群结构的高通量分析

发布时间：2018-03-11 17:17

本文选题：异养硝化-好氧反硝化　切入点：生物多样性　出处：《中国环境科学》2017年05期 　论文类型：期刊论文

【摘要】：为了强化生物反应器脱氮以及揭示微生物菌群结构随时间的动态变化,以Ochrobactrum anthropi XH02和SBR反应器为研究对象,利用16Sr DNA高通量测序技术,对不同时期反应器中微生物的菌群结构和多样性进行了动态分析.研究结果表明,XH02菌的加入使得反应器中TN和COD的去除率分别提升了15%和10%以上;反应器中微生物菌群在属水平上的相对丰度和多样性呈先下降后上升的趋势;XH02的加入对菌群结构产生了较大影响.Acinetobacter、Blvii28和Aquabactenium菌的相对丰度显著下降,而Fontibacter和Treponema菌的相对丰度则在强化过程中显著升高;XH02的相对丰度随着反应器的运行逐渐增加,最后形成了较为稳定的菌群;主成分分析和UPGMA聚类分析大致把反应器运行过程分成4个阶段.
[Abstract]:In order to enhance denitrification in bioreactor and reveal the dynamic change of microbial flora structure with time, Ochrobactrum anthropi XH02 and SBR reactors were used as research objects, and 16Sr DNA high-throughput sequencing technique was used. The microbial community structure and diversity in the reactor at different periods were dynamically analyzed. The results showed that the removal rates of TN and COD in the reactor were increased by more than 15% and 10% respectively with the addition of XH02 bacteria. The relative abundance and diversity of microbial flora in the reactor decreased first and then increased. The addition of XH02 had a great effect on the structure of the flora. The relative abundance of Acinetobacter Blvii28 and Aquabactenium decreased significantly. However, the relative abundance of Fontibacter and Treponema increased with the operation of the reactor, and a stable flora was formed. Principal component analysis (PCA) and UPGMA cluster analysis generally divide the reactor operation into four stages.
【作者单位】：暨南大学环境学院;广东省污染控制与修复材料工程技术中心;
【基金】：国家基金委-广东省政府联合基金重点项目(U1501233) 广东省基金研究团队项目(2016A030312009) 广东省环境污染控制与修复材料工程技术研究中心建设项目(2015B090903070)
【分类号】：X172;X703

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