四溴联苯醚慢性胁迫对大泷六线鱼生长及抗氧化酶活力的影响

发布时间：2018-04-28 18:43

本文选题：四溴联苯醚 + 大泷六线鱼　；参考：《大连海洋大学学报》2017年06期

【摘要】：为研究四溴联苯醚(BDE-47)慢性胁迫对大泷六线鱼Hexagrammos otakii的生物毒性效应,通过设置不同浓度的BDE-47探究了其对大泷六线鱼生长及抗氧化酶活力的影响,试验设置空白对照组和DMSO对照组以及5 ng/L(A组)、50 ng/L(B组)、500 ng/L(C组)、5μg/L(D组)、50μg/L(E组)5个BDE-47浓度组。结果表明:在本试验条件下,各组大泷六线鱼的存活率均为100%;BDE-47对大泷六线鱼生长存在剂量-效应关系,随着BDE-47浓度的增大,大泷六线鱼体长和体质量增长受到的影响也增大,尤其是BDE-47浓度在50 ng/L以上时,鱼体质量出现负增长;BDE-47对大泷六线鱼肝脏和肌肉中的超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力有显著影响,随养殖时间的延长,各浓度组肝脏SOD、CAT活力均呈先升高后降低的波动变化趋势;随着养殖时间的延长,高浓度组肌肉CAT活力呈先升高后降低的变化趋势,低浓度组肌肉CAT活力及各浓度组肌肉SOD活力均无明显变化趋势。研究表明,各浓度的BDE-47不同程度地抑制大泷六线鱼生长,其中体质量较体长对BDE-47的响应更敏感;在肝脏组织中,SOD和CAT活力对于污染物的胁迫反应比在肌肉组织中更敏感。
[Abstract]:In order to study the biological toxicity of four brominated diphenyl ether (BDE-47) chronic stress on Hexagrammos otakii of longlong six line fish, the effects of different concentrations of BDE-47 on the growth of six line fish and antioxidant enzyme activity were investigated. The experiment set up blank control group and DMSO control group and 5 ng/L (A group), 50 ng/L (B group), 500 ng/L (C group), 5 um g/L. (group D), 50 g/L (E group) 5 BDE-47 concentration groups. The results showed that under this test, the survival rate of six line six line fish was 100%; BDE-47 had a dose effect relationship with the growth of six line fish. With the increase of BDE-47 concentration, the growth of the body length and physical quantity of the six line big long longlong was also increased, especially the BDE-47 concentration in 50 ng/L. At the same time, the mass of fish was negatively increased; BDE-47 had a significant influence on the activity of superoxide dismutase (SOD) and catalase (CAT) in the liver and muscles of the six line fish. With the prolongation of the culture time, the SOD of liver and CAT activity in each concentration group increased first and then decreased; with the prolongation of culture time, the high concentration group muscles were increased. The activity of meat CAT increased first and then decreased. There was no obvious change in the muscle CAT activity and the muscle SOD activity in the low concentration group. The study showed that the BDE-47 of each concentration inhibited the growth of the six line fish in different degrees, and the body mass was more sensitive to the response of the body length to the response to BDE-47; in the liver tissue, SOD and CAT vitality was found. The stress response of pollutants is more sensitive than that of muscle tissue.

【作者单位】：大连海洋大学农业部北方海水增养殖重点实验室;大连市水产技术推广总站;
【基金】：国家自然科学基金资助项目(41101481) 辽宁省百千万人才支持计划项目(2014921067)
【分类号】：S917.4

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