紫金牛叶杆菌RC6b及其诱变菌株对二苯砷酸的降解特征

发布时间：2018-05-01 11:37

本文选题：二苯砷酸 + 紫金牛叶杆菌　；参考：《微生物学通报》2017年11期

【摘要】：【目的】阐明紫金牛叶杆菌Phyllobacterium myrsinacearum RC6b及其诱变菌株对二苯砷酸(Diphenylarsinic acid,DPAA)的降解特征与代谢产物。【方法】以紫金牛叶杆菌RC6b为出发菌株,分别以蔗糖、葡萄糖和乙酸钠为外加碳源,优化共代谢降解条件;采用亚硝基胍(N-methyl-N′-nitro-N-nitrosoguanidine,NTG)对出发菌株进行化学诱变,比较诱变前后菌株对DPAA降解能力的变化,鉴定诱变菌株对DPAA的降解代谢产物。【结果】以DPAA为唯一碳源时,培养28 d后RC6b菌株对DPAA的降解率低于2%;分别添加蔗糖、葡萄糖和乙酸钠为外加碳源培养28 d后,DPAA的降解率显著提高,分别达到14.08%、15.21%和15.05%;采用250μg/m L的NTG诱变后获得3株诱变菌,在以DPAA为唯一碳源培养28 d后,3株诱变菌对DPAA的降解率与出发菌株相比均显著提高,其中N-RC6b2对DPAA的降解率最高,达36.71%;代谢产物鉴定结果表明,诱变菌株N-RC6b2对DPAA的代谢产物中有单羟基化DPAA的生成。【结论】RC6b出发菌株难以直接利用DPAA为唯一碳源生长,外加蔗糖、葡萄糖和乙酸钠等共代谢碳源可显著提高菌株RC6b对DPAA的降解率;NTG化学诱变可进一步提高RC6b菌株对DPAA的降解效果,代谢产物为单羟基化DPAA。
[Abstract]:[objective] to elucidate the degradation characteristics and metabolites of Diphenylarsinic acid (DPAA) by Phyllobacterium myrsinacearum RC6b and its mutagenic strains. [methods] with RC6b as the starting strain, sucrose, glucose and sodium acetate were used as additional carbon sources, respectively. The co-metabolic degradation conditions were optimized, and the chemical mutagenesis of the original strain was carried out by using N-methyl-nitro-N-nitro-N-nitro-nitrogen guanidine (NTG), and the degradation ability of the strain to DPAA was compared before and after mutagenesis. [results] when DPAA was used as the sole carbon source, the degradation rate of DPAA was lower than that of RC6b strain after 28 days of culture, and sucrose was added respectively. After cultured with glucose and sodium acetate as carbon source for 28 days, the degradation rate of DPAA increased significantly, reaching 14.08% and 15.05%, respectively, and three strains of mutagenic bacteria were obtained after mutagenesis with 250 渭 g / mL NTG. After being cultured with DPAA as the sole carbon source for 28 days, the degradation rate of DPAA by the three mutagenesis strains was significantly higher than that of the original strain, and the degradation rate of DPAA by N-RC6b2 was the highest (36.71%). The production of monohydroxylated DPAA was found in the metabolites of DPAA by mutagenesis strain N-RC6b2. [conclusion] it is difficult for the original RC6b strain to grow directly using DPAA as the sole carbon source, and sucrose is added to it. Co metabolized carbon sources such as glucose and sodium acetate could significantly increase the degradation rate of DPAA by RC6b. The degradation of DPAA by RC6b could be further improved by chemical mutagenesis. The metabolites were monohydroxylated DPAA.
【作者单位】：中国科学院海岸带环境过程与生态修复重点实验室烟台海岸带研究所;中国科学院大学;
【基金】：国家自然科学基金项目(No.41230858,41571310)~~
【分类号】：X172

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