产絮凝剂菌种的筛选及其在猪场污水净化中的应用

发布时间：2018-05-01 13:24

  本文选题：筛选 + 污水　； 参考：《农业工程学报》2017年20期

【摘要】：分离高效絮凝剂产生菌以有效絮凝猪场污水中的悬浮物。通过稀释平板法和筛选培养基及以发酵液对猪场污水的絮凝率为指标复筛,从猪场污水沉淀池污泥中筛选获得一株产絮凝剂的细菌FD-14,并进行16S r DNA鉴定,然后以絮凝效果为标准,采用La(34)正交设计对其培养条件进行优化;采用单因素试验对其碳氮源进行优化。以最优培养条件下菌株的发酵液实际絮凝猪场污水,与化学絮凝剂的絮凝效果进行比较。分离得到一株高效絮凝剂产生菌FD-14,分子生物学鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens),其产絮凝剂最适条件为温度33℃,p H值6.6,转速150 r/min,培养时间42 h。菌株FD-14利用淀粉作为其廉价碳源替代发酵培养基的絮凝率为80.80%,与原标准培养基差异不显著(P0.05);对猪场实际应用结果表明,微生物絮凝剂和化学絮凝剂对猪场污水絮凝率分别为79.0%和62.7%,微生物絮凝剂比化学絮凝剂对猪场污水絮凝率高16.3%。解淀粉芽孢杆菌FD-14是生物絮凝剂高效产生菌,其产生的絮凝剂可用于猪场污水悬浮物絮凝。
[Abstract]:High-efficiency flocculant-producing bacteria were isolated to effectively flocculate suspended matter from pig farm wastewater. A flocculant producing bacteria FD-14 was obtained by dilution plate method and screening medium, and the flocculation rate of fermentation broth on pig farm sewage was rescreened. A bacterial strain FD-14 was obtained from the sludge of pig farm sewage sedimentation tank, and the 16s r DNA was used to identify the flocculant. Then the culture conditions were optimized by La-34 orthogonal design, and the carbon and nitrogen sources were optimized by single factor test. Under the optimal culture conditions, the actual flocculation of pig wastewater by fermentation broth was compared with that of chemical flocculant. An efficient flocculant producing strain FD-14 was isolated and identified as Bacillus amyloliquefaciensus by molecular biology. The optimum conditions for flocculant production were as follows: temperature 33 鈩，

本文编号：1829554