碳酸氢盐对嗜氢和嗜乙酸产甲烷菌的影响机制

发布时间：2018-05-04 03:38

本文选题：厌氧活性污泥 + 碳酸氢盐　；参考：《中国环境科学》2017年05期

【摘要】：分别以H_2/CO_2和乙酸盐为底物,研究了HCO_3~ 浓度([HCO_3~ ])对厌氧活性污泥中嗜氢产甲烷菌(HM)和嗜乙酸产甲烷菌(AM)活性的影响,并从群落结构、反应动力学和热力学等方面进行了分析.结果表明,0.05~0.20mol/L的[HCO_3~ ]对HM的产甲烷活性具有刺激作用,刺激浓度随着系统氢分压的升高而降低.但高[HCO_3~ ](0.05~0.20mol/L)对AM的产甲烷反应有抑制作用.HM中的Methanobacterium formicium和AM中的Methanosarcina mazei是厌氧活性污泥的优势菌群.随[HCO_3~ ]的升高,M.formicicum的丰度逐渐增加,而M.mazei的丰度则呈现先增加([HCO_3~ ]￡0.10mol/L)后降低([HCO_3~ ]30.15mol/L)的趋势.[HCO_3~ ]可显著改变发酵体系的p H值和产甲烷反应的吉布斯自由能变化,进而影响HM和AM的产甲烷效能.
[Abstract]:Using H_2/CO_2 and acetate as substrates, the effects of HCO3 concentration on the activities of methanophilic bacteria (HM3) and methanophilic acidophilic bacteria (AMAM) in anaerobic activated sludge were studied, and the community structure, reaction kinetics and thermodynamics were analyzed. The results showed that the methanogenic activity of HM was stimulated by 0.05mol / L of 0.20mol / L [HCO3C], and the stimulating concentration decreased with the increase of hydrogen partial pressure. However, high [HCO3] 0.05 (0.20 mol / L) inhibited the methanogenic reaction of AM. The Methanobacterium formicium in HM and Methanosarcina mazei in AM were the dominant flora of anaerobic activated sludge. The abundance of M. formicicum increased gradually with the increase of [HCO3], while the abundance of M.mazei increased first ([HCO3 ~ +] 0.10 mol / L) and then decreased ([HCO3 ~ +] 30.15 mol / L). [HCOS3] could significantly change the pH value of fermentation system and the Gibbs free energy of methanogenic reaction. Furthermore, the methane production efficiency of HM and AM was affected.
【作者单位】：哈尔滨工业大学城市水资源与水环境国家重点实验室;民政部一零一研究所;
【基金】：国家自然科学基金资助项目(51478141) 城市水资源与水环境国家重点实验室(哈尔滨工业大学)自主课题(2016DX06)
【分类号】：X172;X703

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