酿酒废水产微生物絮凝剂菌株的筛选及发酵条件优化

发布时间：2018-05-07 06:51

  本文选题：酿酒废水 + 菌种筛选　； 参考：《中国酿造》2017年09期

【摘要】：该研究采用平板划线法、液体发酵筛选获得产絮凝剂菌株,命名为Y1,采用形态学观察、16S r DNA测序及系统进化树分析进行鉴定;并在单因素试验基础上,采用响应面试验设计方法优化菌株Y1的发酵条件。结果表明,菌株Y1为枯草芽孢杆菌(Bacillus subtilis),最佳发酵条件为初始p H 7.4,转速147 r/min,温度35℃,接种量8%。在最佳发酵条件下,絮凝率为81.6%。
[Abstract]:In this study, a flocculant producing strain, named Y1, was obtained by liquid fermentation, and was identified by morphological observation, 16s r DNA sequencing and phylogenetic tree analysis. The fermentation conditions of strain Y1 were optimized by response surface design. The results showed that strain Y1 was Bacillus subtilisa. The optimum fermentation conditions were as follows: initial pH 7.4, rotational speed 147 rmin, temperature 35 鈩，

本文编号：1855849