脱色菌群的富集及其对活性艳红X-3B的脱色效果

发布时间：2018-05-13 20:09

  本文选题：定向驯化 + 脱色菌群　； 参考：《安徽农业大学学报》2017年05期

【摘要】：通过定向驯化法,从实验室活性污泥处理系统中,富集驯化出对活性艳红X-3B具有良好脱色性能的菌群H1~H5,针对其染料脱色性能进行了一系列研究。结果表明,脱色菌群有一定的耐盐能力,在盐度为15%,染料浓度100 mg·L~(-1)时,24 h后脱色菌群H5的脱色率为86%;脱色菌群H1~H5在低染料浓度下能够快速脱色(600 mg·L~(-1)),在800~1 500 mg·L~(-1)的浓度范围内,经过48 h的培养后,脱色率都能达到95%以上;同时染料浓度为500 mg·L~(-1)时,脱色菌群H5脱色的适宜p H5~11,温度25~37℃,培养时间24 h,脱色率达到90%以上;脱色菌群H5对直接大红4BS和分散艳蓝KN-R有着良好的脱色性能,培养24 h,脱色率达到90%以上。利用扫描电镜观察,发现脱色菌群H5以杆菌为主。基于Illumina Miseq测序平台研究了脱色菌群H5的微生物菌群,发现其中优势菌属为Alcaligenes sp.和Pseudomonas sp.,所占比例分别为55.78%和11.14%。
[Abstract]:Through the directional acclimation method, the bacteria H _ 1 / H _ 5which has good decolorization performance to reactive brilliant red X-3B was enriched and domesticated from the activated sludge treatment system in laboratory. A series of studies were carried out on its dye decolorization performance. The results showed that the decolorization bacteria had a certain salt tolerance, and the decolorization rate of H5 was 86 after 24 h at the salinity of 15 and the dye concentration of 100mg / L ~ (-1), and the decolorization rate of decolorization bacteria H1~H5 could be quickly decolorized at a concentration of 600mg / L ~ (-1), within the concentration range of 8001 500 mg / L ~ (-1) at low dye concentration. After 48 h culture, the decolorization rate was more than 95%, and the decolorization rate was over 90% when the dye concentration was 500 mg / L 路L ~ (-1). The suitable decolorization rate of the decolorizing bacteria H _ 5 was over 90%, temperature 2537 鈩，

本文编号：1884626