自清洗式活性生物滤池对工业废水深度处理的试验研究
本文选题:自清洗式活性生物滤池 + 滤料 ; 参考:《郑州大学》2017年硕士论文
【摘要】:现阶段水资源短缺和水污染情况越来越严重,污水处理排放的标准越来越高,有些污水处理厂二级生化出水已经不能满足《城镇污水处理厂污染物排放标准》(GB 18918—2002)一级A标准的排放要求,所以需要增加深度处理设施;自清洗式活性生物滤池具有操作简单、占地面积小的特点,在深度处理脱氮作用方面有广阔的应用前景。本次试验采用自然挂膜和人工接种污泥法挂膜两种启动方式来运行,对比不同滤料在自然挂膜条件下对污染物的去除效果,并在人工接种污泥条件下优化碳源投加种类和投加量,运用高通量测序法对滤池内部和生化池内微生物进行分析,确定滤池内优势菌种,并将滤池和水厂生化池内微生物进行对比分析。(1)自然挂膜启动阶段周期运行20天,通过对比两种滤料对COD、TN、TP的去除效果研究得出结论:石英砂对污染物的整体去除效果高于陶粒。(2)人工接种污泥条件下,乙酸钠对COD的去除率范围是33%~39%,乙醇的去除率范围为39.2%~46.1%,葡萄糖的则为27.3%~41.2%,乙醇TN的去除率范围是48.6%~80%,乙酸钠和葡萄糖去除范围则分别为34.4%~39.9%,35.4%~58.8%,确定乙醇为最佳投加碳源种类。研究乙醇的最佳投药量和最适C/N,向试验进水中投加不同量的乙醇,分析其污染物的处理效果,得出乙醇最佳投药量为50ml/m3,最适宜本次试验的C/N为3,最佳HRT为60min。(3)对样品中微生物进行丰度分析,β-变形菌纲(Betaproteobacteria)为主要微生物种群,许多种类都具有反硝化能力,而在生化池中疣微菌门(Verrucomicrobia)、酸杆菌门(Acidobacteria)含量相对较多,为好氧型细菌;在科分类水平上分析,含量较高的微生物菌科为红环菌科(Rhodo-cyclaceae),生化池红环菌科(Rhodo-cyclaceae)含量低于滤池,在属分类水平上分析可得,样品中的优势菌种为Thauera菌属,它们均是反硝化反应的主要参与者。(4)对自清洗式活性生物滤池和水厂生化池五个样品做Alpha多样性分析、OTU韦恩图分析及OTU Rank曲线,从中可以看出微生物物种均比较丰富,具有多样性,但滤池微生物数量高于生化池。
[Abstract]:At the present stage, the shortage of water resources and water pollution are becoming more and more serious, and the standards of sewage treatment and discharge are becoming higher and higher. In some wastewater treatment plants, the secondary biochemical effluent can no longer meet the discharge requirements of the first class A standard of "pollutants discharge Standard of the Municipal sewage treatment Plant" (GB 18918-2002), so it is necessary to increase the advanced treatment facilities. Self-cleaning active biological filter has the characteristics of simple operation and small area. It has a broad application prospect in deep treatment of denitrification. In this experiment, two kinds of start-up methods, natural membrane hanging and artificial inoculating sludge method, were used to run, and the effects of different filter media on the removal of pollutants under the condition of natural membrane suspension were compared. Under the condition of artificial inoculation sludge, the species and dosage of carbon source were optimized, and the microbes in the filter and biochemical tank were analyzed by high-throughput sequencing method, and the dominant bacteria in the filter were determined. The microbes in the filter tank and the biochemical tank of water plant were compared and analyzed. By comparing the removal effect of two kinds of filter media on COD ~ (2 +) TNN _ (TP), it is concluded that the overall removal efficiency of quartz sand is higher than that of ceramsite. 2) under the condition of artificial inoculation sludge, The removal rate of sodium acetate to COD is 339.39, the removal rate of ethanol is 39.2 and 46.1s, the range of glucose is 27.31.2, the removal rate of ethanol TN is 48.6 and 800.The removal range of sodium acetate and glucose is 39.935.435.4 and 58.8respectively, which determines that ethanol is the best kind of carbon source. To study the optimal dosage of ethanol and the optimum C / N, to add different amounts of ethanol to the experimental influent, and to analyze the treatment effect of the pollutants. The results show that the optimum dosage of ethanol is 50ml / m ~ 3, the best C / N is 3 and the best HRT is 60 min 路m ~ (3), and 尾 -Proteobacteriae (Beta proteobacteriae) is the main microbial population, and many species have denitrification ability. However, the content of Acidobacterium acidobacteriae was relatively high in the biochemical pool, and the content of Rhodo-cyclaceae, Rhodo-cyclaceaeae, was lower than that of the filter, at the taxonomic level of the family Rhodo-cyclaceaeae, which was higher than that in the filter, and the content of Acidobacteriae in the biochemistry pool was lower than that in the filter, and the content of Rhodo-cyclaceaeae in the biochemistry pool was lower than that in the filter, and in the taxonomic level, the microbiota was Rhodo-cyclaceaeae. At the taxonomic level, the dominant species in the sample were Thauera. They are the main participants in denitrification. (4) the Alpha diversity analysis and OTU Rank curves of five samples from self-cleaning active biofilter and water plant biochemistry tank were analyzed. It can be seen that the microbial species are abundant. It has diversity, but the number of microorganism in filter is higher than that in biochemical pond.
【学位授予单位】:郑州大学
【学位级别】:硕士
【学位授予年份】:2017
【分类号】:X703
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