ANAMMOX的快速启动及EPS在ANAMMOX颗粒污泥中的空间分布

发布时间：2018-05-23 17:58

本文选题：厌氧氨氧化 + 颗粒污泥　；参考：《环境科学》2017年07期

【摘要】：为探讨厌氧氨氧化反应的快速启动过程及胞外聚合物(extracellular polymeric substances,EPS)在厌氧氨氧化颗粒污泥中的空间分布,采用厌氧序批式反应器(anaerobic sequencing batch reactor,ASBR)接种活性污泥成功启动厌氧氨氧化反应.结果表明稳定运行时,NH_4~+-N、NO_2~--N去除率均达到99%以上,TN去除率为89.87%±0.43%,总氮(TN)去除负荷达到1.7kg·(m~3·d)~(-1).NH_4~+-N与NO_2~--N的消耗量和NO_3~--N生成量之间的比例关系为1∶(1.32±0.08)∶(0.24±0.03).反应器运行中,出水pH和NO_3~--N浓度可作为反应性能的指标,快速判断反应器运行情况.蛋白质为厌氧氨氧化颗粒污泥EPS的主要组分,蛋白质(PN)和多糖(PS)的含量分别为(59.61±5.64)mg·g~(-1)、(12.21±2.04)mg·g~(-1),PN/PS为4.88±1.39.β-D-呋喃葡萄糖和死细胞集中分布在颗粒污泥最外层;活细胞、蛋白质、脂类、α-呋喃葡萄糖和α-甘露糖遍布整个颗粒污泥,但主要集中在外侧.蛋白质和脂类构成了厌氧氨氧化颗粒污泥的骨架,厌氧氨氧化菌分布在蛋白质和脂类中间.
[Abstract]:In order to investigate the rapid start-up process of anaerobic ammonia oxidation reaction and the spatial distribution of extracellular polymeric substrates in anaerobic ammonia oxidized granular sludge, anaerobic sequencing batch reactor (anaerobic sequencing batch reactor) was used to inoculate the activated sludge to successfully initiate the anaerobic ammonia oxidation reaction. The results showed that the removal rate of total nitrogen (TN) was 89.87% 卤0.43, and the ratio of total nitrogen removal load to NO_2~--N consumption and NO_3~--N production was 1: 1. 32 卤0. 08% 卤0. 24 卤0. 03%, respectively. The results showed that the removal rates of NH4 ~ -N were more than 99%, 89. 87% 卤0. 43%, and the ratio of total nitrogen removal load to NO_2~--N was 1: 1.32 卤0. 08% 卤0. 24 卤0. 03%. The pH of effluent and the concentration of NO_3~--N can be used as the indexes of reaction performance, and the operation condition of the reactor can be judged quickly during the operation of the reactor. Protein was the main component of EPS in anaerobic ammonia oxidized granular sludge. The contents of protein PNs and polysaccharides PNs were 59.61 卤5.64)mg / g ~ (-1) and 12.21 卤2.04)mg / g ~ (-1) P _ (P) = 4.88 卤1.39 respectively. 尾 -Dfuran glucose and dead cells were mainly distributed in the outermost layer of granular sludge. Lipids, 伪-furan glucose and 伪-mannose were found throughout the granular sludge, but mainly on the outside. Proteins and lipids form the framework of anaerobic ammoxidation granular sludge, and anaerobic ammonia-oxidizing bacteria distribute between proteins and lipids.
【作者单位】：西安建筑科技大学环境与市政工程学院;西北水资源与环境生态教育部重点实验室;浙江工业大学环境学院;
【基金】：陕西省住房城乡科技开发项目(2015-K65) 浙江省自然科学青年基金项目(LQ15E080011)
【分类号】：X703.1

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