外接菌种对污泥堆肥效能及堆体细菌群落的影响

发布时间：2018-05-28 08:11

本文选题：高温菌 + 污泥堆肥　；参考：《环境科学》2017年08期

【摘要】：以污泥、锯末、蘑菇渣为堆肥原料,分阶段添加高温复合菌和白腐真菌,通过测定温度、pH、有机质、水溶性有机碳、含水率、总氮、NH_4~+-N、NO_3~--N和种子发芽率,比较了外接菌种对堆肥效能的影响.利用高通量测序技术,研究了污泥堆肥过程中细菌群落结构变化及外接菌种对细菌群落的影响.结果表明,外接菌种可以延长堆体高温持续时间,降低氮损失,加快堆体腐熟脱毒.在整个堆肥过程中,细菌群落结构发生了较大的演变,同一堆肥的不同阶段的细菌群落结构相似性较低,同一时期不同堆肥的细菌群落结构相似性较高.外接菌种提高了堆体中细菌群落的丰富度,增加了高温期优势菌的所占比例,但未改变优势菌的种类.典型对应分析(CCA)表明pH对细菌群落结构的影响最大,温度主要与9个菌属呈正相关关系.
[Abstract]:Using sludge, sawdust and mushroom dregs as composting materials, high temperature compound fungi and white rot fungi were added in different stages. The pH of temperature, organic matter, water soluble organic carbon, moisture content, total nitrogen (NH4 ~ -N) no _ 3 -N and seed germination rate were determined. The effect of external bacteria on composting efficiency was compared. The changes of bacterial community structure during sludge composting and the effect of inoculated bacteria on bacterial community were studied by high-throughput sequencing technique. The results showed that the external bacteria could prolong the duration of high temperature, reduce the nitrogen loss and accelerate the detoxification. In the whole composting process, the bacterial community structure changed greatly. The similarity of bacterial community structure was lower in different stages of the same compost and higher in the same composting period. The external species increased the richness of bacterial community and the proportion of dominant bacteria in high temperature period, but did not change the species of dominant bacteria. Canonical correspondence analysis (CCAA) showed that pH had the greatest influence on the community structure of bacteria, and the temperature had a positive correlation with 9 genera.
【作者单位】：华南理工大学环境与能源学院工业聚集区污染控制与生态修复教育部重点实验室广东省环境风险防控与应急处置工程技术研究中心;暨南大学化学系;
【基金】：广东省科技厅公益研究与能力建设专项重点项目(2015B020215006) 广州市科信局产学研协同创新重大专项(2014Y2-00523)
【分类号】：X172;X703

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