邻苯二甲酸二甲酯暴露对凡纳滨对虾壳膜几丁质酶的影响

发布时间：2018-05-28 21:15

本文选题：凡纳滨对虾 + 邻苯二甲酸二甲酯　；参考：《江苏农业科学》2017年17期

【摘要】：以凡纳滨对虾为试验对虾,通过不同浓度0、2、10、20μg/L邻苯二甲酸二甲酯(DMP)暴露不同时间(120、240 h)后,采用酶促反应动力学法及实时PCR检测分析对虾壳膜几丁质酶比活力及几丁质酶mRNA表达量差异。结果显示,DMP暴露对对虾壳膜几丁质酶比活力具有抑制作用,但不具有显著性。不同浓度DMP暴露不同时间后,对对虾壳膜几丁质酶系列基因的mRNA表达量均受到不同程度的影响,2μg/L DMP暴露120 h时,对虾壳膜Lv CHT3mRNA表达量显著上升;2μg/L DMP暴露240 h时,Lv CHT2 mRNA表达量显著下降;20μg/L DMP暴露240 h时,Lv CHT6 mRNA表达量也显著下降,说明DMP对对虾生长发育具有毒性。
[Abstract]:Using Penaeus vannamei as an experimental shrimp, after being exposed to DMP20 渭 g / L of dimethyl phthalate for 120240 h at different concentrations, The specific activity of chitinase and the difference of chitinase mRNA expression in shrimp shell membrane were analyzed by enzymatic kinetic method and real-time PCR detection. The results showed that the specific activity of chitinase in shrimp shell membrane was inhibited by DMP exposure, but it was not significant. After exposure to different concentrations of DMP for different time, the mRNA expression of chitinase genes in shrimp shell was affected by different degrees of exposure to 2 渭 g / L DMP for 120h. The expression of Lv CHT3mRNA in shrimp shell membrane was significantly increased with 2 渭 g / L DMP exposure for 240h. The expression of Lv CHT2 mRNA in shrimp shell membrane decreased significantly when exposed to 20 渭 g / L DMP for 240h, indicating that DMP is toxic to the growth and development of Penaeus penaeus.
【作者单位】：泉州师范学院化工与材料学院;
【基金】：国家自然科学基金青年科学基金(编号:31302190) 福建省自然科学基金(编号:2012J01047) 福建省泉州市优秀人才培养专项(编号:16D04)
【分类号】：S917.4;X503.22

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