耐盐植物虎尾草内生解烃细菌的筛选及其降解特性

发布时间：2018-05-29 16:24

本文选题：石油污染 + 盐渍化土壤　；参考：《农业环境科学学报》2017年11期

【摘要】：从耐盐植物中分离具有石油烃降解功能内生细菌并研究其降解特性,为内生细菌协同宿主植物修复石油污染盐渍化土壤提供基础。以黄河三角洲长期受石油污染重盐碱地生长的耐盐植物虎尾草为材料,分离出能以柴油为唯一碳源生长的内生细菌24株。通过柴油降解试验,筛选得到1株高效石油烃降解内生细菌BF04。经生理生化特性及16S r DNA同源序列分析,确定BF04为短小芽孢杆菌(Bacillus pumilus)。通过液体培养试验研究了BF04对不同烃的利用能力和对柴油的降解动力学特征。结果表明:BF04能够利用直链烷烃、支链烷烃和单环芳烃,不能利用多环芳烃和苯酚。BF04能降解柴油中大部分烷烃,其中对短链烷烃(n C11~n C23)降解率高于长链烷烃(n C24~n C27)。BF04对柴油降解可以用一级反应动力学模型表示。在含有3%Na Cl的液体培养基中,BF04具有较强的降解柴油能力,在修复石油污染高盐环境中具有一定的应用潜力。
[Abstract]:Endophytic bacteria with petroleum hydrocarbon degradation function were isolated from salt-tolerant plants and their degradation characteristics were studied, which provided the basis for the remediation of petroleum-contaminated salinized soil by endophytic bacteria in cooperation with host plants. In this paper, 24 strains of endophytic bacteria which can grow with diesel oil as the sole carbon source were isolated from the salt-tolerant plant of the Yellow River Delta, which grew in heavy saline and alkali soil polluted by oil for a long time. A high efficiency petroleum hydrocarbon degrading endophytic bacteria BF04 was obtained by diesel oil degradation test. Based on physiological and biochemical characteristics and 16s r DNA homology analysis, BF04 was identified as Bacillus pumilusus. The utilization ability of BF04 to different hydrocarbons and the degradation kinetics of diesel oil were studied by liquid culture. The results show that: BF04 can use straight chain alkanes, branched alkanes and monocyclic aromatic hydrocarbons, and can not use polycyclic aromatic hydrocarbons and phenol. BF04 can degrade most of the alkanes in diesel oil. The degradation rate of short chain alkanes n C 11 C 23) is higher than that of long chain alkanes n C 24 n C27).BF04. The degradation rate of diesel oil can be expressed by the first order reaction kinetic model. In liquid medium containing 3%Na Cl, BF04 has a strong ability to degrade diesel oil, and has a certain application potential in the remediation of high salt environment caused by oil pollution.
【作者单位】：滨州学院生物工程学院山东省黄河三角洲野生植物资源开发利用工程技术研究中心;滨州学院山东省黄河三角洲生态环境重点实验室;滨州职业学院生物工程学院;
【基金】：国家自然科学基金青年科学基金项目(41401359) 山东省高等学校科技计划项目(J14LE05) 山东省自然科学基金项目(ZR2013DM001)~~
【分类号】：X172

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