伊乐藻-高效脱氮微生物协同作用对污染水体氮素脱除机制的影响

发布时间：2018-06-04 10:54

本文选题：伊乐藻-固定化高效脱氮微生物联用技术 + 铵态氮　；参考：《环境科学》2017年11期

【摘要】：在入贡湖河道亲水河采集上覆水、伊乐藻及底泥柱芯样品进行室内实验,探究伊乐藻与固定化高效脱氮微生物协同作用下脱除铵态氮的机制,以及对污染水体的生态修复效果.运用~(15)N同位素配对技术及高通量测序技术,研究了4个不同处理组中NH_4~+的转化机制,其中,处理组A:裸泥组,处理组B:固定化高效脱氮微生物,处理组C:伊乐藻,处理组D:固定化高效脱氮微生物+伊乐藻.结果表明,氮素脱除主要有底泥存储、伊乐藻吸收及微生物过程(反硝化和厌氧氨氧化)这3种途径.在添加沉水植物伊乐藻的处理组C和D中,伊乐藻对~(15)NH_4~+吸收率分别为25.44%和19.79%.不同处理组中底泥对~(15)NH_4~+存储率分别为7.94%、5.52%、6.47%和4.86%,微生物过程以气体形式释放的~(15)NH_4~+分别为16.06%、28.86%、16.93%和33.09%.反硝化和厌氧氨氧化是产生含氮素气体的主要过程,对于处理组D,脱氮微生物丰度和多样性均得到不同程度的提升.4个不同处理组对~(15)NH_4~+的总去除率分别为24%、34.38%、48.84%和57.74%,伊乐藻与高效脱氮微生物联用技术(EINCB)的应用,可以提高水体氮素的脱除速率,促进污染水体的净化.
[Abstract]:The overlying water was collected from the hydrophilic river of Jingong Lake, and the samples of Eloidea and sediment core were collected in laboratory. The mechanism of removing ammonium nitrogen under the synergistic action of Eloidea and immobilized high efficiency denitrification microorganism, and the effect of ecological remediation on polluted water were explored. The transformation mechanism of NH _ 4 ~ in four different treatment groups was studied by using the technique of N isotopic pairing and high-throughput sequencing. Among them, treatment group A: bare mud group, treatment group B: immobilized high-efficiency denitrification microorganism, treatment group C: Eloidea. Treatment group D: immobilized high efficiency denitrogenation microorganism Eloidea. The results showed that nitrogen removal mainly includes sediment storage, Eloidea absorption and microbial processes (denitrification and anaerobic ammonia oxidation). In the treatment groups C and D, the absorptivity was 25.44% and 19.79%, respectively. In different treatment groups, the storage rates of NH4 ~ in the sediment were 7.94 ~ 5.52% and 6.47% and 4.86%, respectively. The NH4 ~ of 15 ~ + ~ was 16.06% and 33.09%, respectively, which were released in the form of gas from the microbial process. Denitrification and anaerobic ammonia oxidation are the main processes to produce nitrogen-containing gases. For treatment group D, the abundance and diversity of denitrogenation microorganism were improved in different degrees. The total removal rate of NH4 ~ was 244.38% and 57.74%, respectively. The application of EINCBs to EINCBs was studied. It can improve the rate of nitrogen removal and promote the purification of polluted water.
【作者单位】：南京大学环境学院污染控制与资源化研究国家重点实验室;
【基金】：国家水体污染控制与治理重大专项(2013ZX07101-014)
【分类号】：X172;X52

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