市政污泥接种焦化废水好氧降解能力及微生物群落演替的响应分析

发布时间：2018-06-05 14:56

本文选题：焦化废水 + 市政污泥　；参考：《环境科学》2017年09期

【摘要】：焦化废水是毒性很大的典型工业废水,生物处理过程中需要微生物具备很强的适应能力.以探讨焦化废水对微生物的毒性抑制以及微生物对焦化废水的适应过程为目的,通过于焦化废水原水中接种市政污泥,在考察COD、苯酚、氨氮和硫氰化物等主要污染物指标降解的基础上,运用Illumina高通量测序平台分析降解过程微生物群落组成及多样性变化的响应关系.结果表明,接种了市政污泥的焦化废水培养16 h后COD开始下降,40 h时苯酚降解了97.14%,72 h时硫氰化物开始降解,96 h时硫氰化物浓度低于检测限,氨氮浓度随着硫氰化物的降解而升高.群落测序分析表明,不同培养阶段污泥中微生物表现出群落结构及丰度上的差异:在苯酚降解阶段,苯酚优势降解菌Acinetobacter、Pseudomonas的丰度增大,48 h总相对丰度为13.04%;在硫氰化物降解阶段,Sphingobacterium、Brevundimonas、Lysobacter、Chryseobacterium为主导菌属,96 h时其总相对丰度为16.13%;在144 h阶段,优势菌属则变为Fluviicola、Stenotrophomonas和Thiobacillus,总相对丰度为22.45%.由此认为,市政污泥克服了焦化废水中毒性成分的抑制作用之后能迅速适应环境,表现出微生物群落结构随着废水降解成分的变化而改变,环境因子和降解功能菌之间的竞争是群落结构演变的主要因素.
[Abstract]:Coking plant wastewater is a typical industrial wastewater with high toxicity. It is necessary for microorganisms to have strong adaptability in the process of biological treatment. In order to study the inhibition of microbial toxicity of coking wastewater and the adaptation of microorganisms to coking wastewater, municipal sludge was inoculated in raw wastewater of coking plant, and COD and phenol were investigated. Based on the degradation of main pollutants such as ammonia nitrogen and thiocyanide, the response relationship of microbial community composition and diversity changes during degradation was analyzed by Illumina high-throughput sequencing platform. The results showed that the COD of coking wastewater inoculated with municipal sludge decreased at 40 h after incubation, and the degradation of phenol was 97.14 h and the concentration of thiocyanate was lower than the detection limit at 96 h when thiocyanate was degraded at 72 h. The concentration of ammonia nitrogen increased with the degradation of thiocyanate. Community sequencing analysis showed that the microbial community structure and abundance were different in different culture stages: in phenol degradation stage, The total relative abundance of acinetobacter Pseudomonas in 48 h was 13.04, the total relative abundance of Sphingobacter Brevundimonia Lysobacter Chryseobacterium was 16.1313 at 96 h, and at 144 h, the dominant genus became Fluviicolaena Stenotrophomonas and Thiobacillus. the total relative abundance was 22.45. It is concluded that municipal sludge can quickly adapt to the environment after overcoming the inhibition of toxic components in coking wastewater, and show that the microbial community structure changes with the change of degradation composition of wastewater. Competition between environmental factors and biodegradable functional bacteria is the main factor in the evolution of community structure.
【作者单位】：华南理工大学生物科学与工程学院;广州市市政工程维修处;华南理工大学环境与能源学院;
【基金】：国家自然科学基金项目(21377040) 广东省省级科技计划项目(2015A020215008) 广东省应用型科技研发专项(2015B020235005)
【分类号】：X172;X784

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