北海湖微生物群落结构随季节变化特征

发布时间：2018-06-19 10:47

本文选题：北海湖 + 高通量测序　；参考：《环境科学》2017年08期

【摘要】：分别于夏季、秋季、冬季和春季采集北海湖水样,进行水质和微生物群落结构分析.水质分析表明,夏季和秋季的水质较冬季和春季差,其中TN和TP是影响水质的最重要因素.通过Mi Seq高通量测序分析微生物群落结构表明:蓝细菌门(Cyanobacteria)、变形菌门(Proteobacteria)、放线菌门(Actinobacteria)和拟杆菌门(Bacteroidetes)是湖水中的优势门,但在不同季节的分布不同.夏季和秋季微生物的多样性高,蓝细菌门(Cyanobacteria)是第一优势菌门(32.7%~43.2%),其中聚球藻属(Synechococcus)是优势蓝细菌.冬季变形菌门(Proteobacteria)的含量相对丰富,占细菌群落的34.8%~40.4%.而春季,放线菌门(Actinobacteria)和拟杆菌门(Bacteroidetes)的含量增加,两者之和占细菌群落的51.5%~64.3%.RDA(redundancy analysis)分析表明,水温是影响北海湖微生物群落结构的最主要环境因子.总体而言,北海湖水质符合景观水的标准,但夏季聚球藻属(Synechococcus)的大量繁殖使北海湖发生水华的风险增加,因此要加强对北海湖水质检测和管理.
[Abstract]:Water samples from Beihai Lake were collected in summer, autumn, winter and spring to analyze water quality and microbial community structure. Water quality analysis shows that the water quality in summer and autumn is worse than that in winter and spring, among which TN and TP are the most important factors affecting water quality. The analysis of microbial community structure by Mi Seq high-throughput sequencing showed that Cyanobacteria, Proteobacteria, Actinobacteria) and Bacteroideteswere the dominant ones in lake water, but the distribution was different in different seasons. Cyanobacter teriana is the first dominant bacterium in summer and autumn, and Synechococcus is the dominant cyanobacteria. The content of Proteobacteria is relatively rich in winter, which accounts for 34.8g and 40.4in the community of bacteria. However, in spring, the contents of Actinobacteria) and Bacteroidetes increased, and the sum of them accounted for 51.5% of the total bacterial community. The results of water temperature analysis showed that water temperature was the most important environmental factor affecting the microbial community structure in Beihai Lake. In general, the water quality of Beihai Lake meets the standard of landscape water, but the risk of occurrence of Shui Hua in Beihai Lake is increased due to the proliferation of Synechococcus in summer. Therefore, it is necessary to strengthen the monitoring and management of water quality in Beihai Lake.
【作者单位】：中国科学院大学资源与环境学院;华北水利水电大学水务研究院;
【基金】：中国科学院重点布署项目(KZZ-EW-09-01)
【分类号】：X172;X52

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