异戊二烯对刺参急性毒性及其抗氧化酶和免疫酶活性的影响

发布时间：2018-08-06 09:50

【摘要】：为研究有机污染物对海洋生物的毒性影响,选择海洋环境中典型的异戊二烯作为暴露污染物,研究其对体质量为(5.23±0.96)g刺参Apostichopus japonicus的急性毒性及其体腔液中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、酸性磷酸酶(ACP)和碱性磷酸酶(AKP)活性的影响,并在实验室条件下,采用静水式生物毒性试验法开展了急性毒性试验,以及96 h亚致死效应的毒性试验。结果表明:异戊二烯对刺参的24、48、72、96 h半致死质量浓度分别为83.00、78.09、72.93、69.77 mg/L,安全质量浓度为6.98 mg/L;亚致死试验中,CAT活性受到的影响最大,表现为受到抑制作用,存在良好的剂量-效应和时间-效应正相关性;96 h时,中、低浓度异戊二烯对SOD活性具有显著的诱导作用(P0.05),而高浓度异戊二烯对SOD活性具有极显著的抑制作用(P0.01);ACP和AKP活性受到的影响较小,但48 h时两种酶均受到极显著的诱导作用(P0.01),96 h时AKP活性受到显著的诱导作用(P0.05);随着异戊二烯质量浓度的增加和时间的延长,刺参体腔液中4种酶活性受到不同程度的影响,因此,异戊二烯胁迫对刺参体内产生自由基导致氧化胁迫效应,以及对消化、能量代谢系统的影响可能是其重要的致毒原因,刺参体腔液中4种酶的变化体现了异戊二烯的致毒机制。本研究结果可为异戊二烯对海洋生物的生态毒理学方面的研究提供参考依据。
[Abstract]:In order to study the toxic effects of organic pollutants on marine organisms, the typical isoprene in the marine environment was selected as exposure pollutants. The acute toxicity of Apostichopus japonicus and the activities of superoxide dismutase (SOD),) catalase (CAT),) acid phosphatase (ACP) and alkaline phosphatase (AKP) in the body cavity of Acanthopsis japonicus were studied. The acute toxicity test and the 96 h sublethal toxicity test were carried out by hydrostatic biotoxicity test. The results showed that the semi-lethal concentration of isoprene to sea cucumber was 83.00 (78.09) (72.93) 渭 g / L and the safe concentration was 6.98 mg / L, respectively, and the activity of catalase was the most affected in the sublethal test, which showed that the activity of catalase was inhibited, and the concentration of isoprene was 69.77 mg / L and 6.98 mg / L, respectively, in the sublethal test. There was a good dose-effect and time-effect positive correlation at 96 h. Low concentration isoprene could induce SOD activity significantly (P0.05), while high concentration isoprene had extremely significant inhibitory effect on SOD activity (P0.01). However, at 48 h, both of the two enzymes were significantly induced (P0.01) and the activity of AKP was significantly induced at 96 h (P0.05), and with the increase of isoprene concentration and the prolongation of time, the activities of four enzymes in the body cavity fluid of Acanthopsis japonicus were affected to some extent. Therefore, the effects of isoprene stress on the production of free radicals in the body of Acanthopsis japonicus and the effects of isoprene stress on digestion and energy metabolism may be important causes of its toxicity. The changes of four enzymes in the body cavity of Acanthopsis japonicus showed the toxic mechanism of isoprene. The results of this study may provide a reference for the study of ecotoxicology of isoprene to marine organisms.
【作者单位】：辽宁省海洋水产科学研究院辽宁省海洋生物资源和生态学重点实验室;大连市水产研究所;
【基金】：辽宁省海洋与渔业厅科研项目(201417)
【分类号】：X171.5

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