同步脱氮除磷好氧颗粒污泥培养过程微生物群落变化

发布时间：2018-08-11 17:59

【摘要】：本实验利用生活污水培养具有同步脱氮除磷(simultaneous nitrogen and phosphorus removal,SNPR)功能的好氧颗粒污泥(aerobic granular sludge,AGS).采用Illumina Mi Seq PE300高通量测序对AGS培养过程中细菌群落变化进行了研究,以期揭示污泥好氧颗粒化成因.采用实时荧光定量PCR对AGS培养过程中氨氧化细菌(ammonia-oxidizing bacteria,AOB)、氨氧化古菌(ammonia-oxidizing archaea,AOA)、亚硝酸盐氧化菌(nitrite-oxidizing bacteria,NOB)和聚磷菌(polyphosphate accumulating organisms,PAOs)的丰度变化进行了研究.结果表明:历时100 d培养出的AGS质地紧实,具有良好的SNPR效果.AGS胞外聚合物(extracellular polymeric substances,EPS)中多糖含量在培养过程中增加明显,而蛋白质含量保持稳定.AGS培养过程中,AOB的丰度略微下降,AOA的丰度明显下降,NOB的丰度无明显变化,而PAOs的丰度在AGS培养初期明显增加.在AGS培养过程中,细菌群落多样性呈现出先升高后降低的趋势,且细菌群落组成发生了明显的变化.持久型OTUs占样品总序列数的92.70%,其中变形菌门(Proteobacteria,31.07%~53.67%)、拟杆菌门(Bacteroidetes,6.70%~16.50%)和绿弯菌门(Chloroflexi,7.84%~13.36%)是AGS培养过程中的细菌优势门.Candidatus competibacter属在AGS培养过程中大量富集(由种泥中的0.11%增加到35.33%),其可能会分泌胞外多糖,形成黏性EPS,促进絮状污泥团聚成为AGS.
[Abstract]:In this experiment, aerobic granular sludge (aerobic granular sludgege AGS) with simultaneous nitrogen and phosphorus removal (simultaneous nitrogen and phosphorus removalSNPR was cultured from domestic sewage. The changes of bacterial community during AGS culture were studied by Illumina Mi Seq PE300 high-throughput sequencing in order to reveal the cause of aerobic granulation of sludge. The changes of abundance of ammonia-oxidizing bacteria (ammonia-oxidizing), ammonia-oxidizing archaea (AOA), nitrite-oxidizing bacteria (nitrite-oxidizing) and (polyphosphate accumulating organismsPAOs (polyphosphate accumulating organismsPAOs) during AGS culture were studied by real-time fluorescence quantitative PCR. The results showed that the content of polysaccharides in the extracellular polymer (extracellular polymeric substanceses EPS) of AGS, which was cultured for 100 days, was compact and had a good effect of SNPR. However, the abundance of AOB decreased slightly during the culture of AGS, but the abundance of PAOs increased significantly at the initial stage of AGS culture. In the process of AGS culture, the diversity of bacterial community increased first and then decreased, and the composition of bacterial community changed obviously. Persistent OTUs accounts for 92.70% of the total sequence of samples, of which Proteobacteriae 31.070.57%, Bacteroides 6.70% and Chloroflexius 7.84% 13.36% are abundant in the AGS culture process (from 0.11% to 35.33% of the mud). May secrete an exopolysaccharide, The formation of viscous EPSs promotes agglomeration of flocculated sludge into AGSs.
【作者单位】：北京工业大学环境与能源工程学院城镇污水深度处理与资源化利用技术国家工程实验室;北京城市排水集团有限责任公司科技研发中心;
【基金】：国家自然科学基金项目(51378027,51578015) 北京市自然科学基金项目(8162010)
【分类号】：X172;X703

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