非致死剂量的四溴双酚A胁迫下蚯蚓的生长和抗氧化防御反应

发布时间：2018-10-08 07:38

【摘要】：以自然土壤为介质,考察赤子爱胜蚓(Eisenia fetida)的生长抑制率、蛋白质含量、超氧化物歧化酶(SOD)活性、谷胱甘肽转移酶(GST)活性等指标,进行了急性(14 d)、亚急性(28 d)暴露下四溴双酚A(tetrabromobisphenol A,TBBPA)非致死剂量胁迫对蚯蚓的生长和抗氧化防御效应研究。结果表明:在急性试验中TBBPA对蚯蚓的生长抑制均存在显著的剂量效应关系,而在亚急性暴露期,400 mg·kg~(-1)时显著抑制蚯蚓的生长(P0.05);TBBPA对蚯蚓体内蛋白含量影响,在急性试验中各处理间差异不显著(P=0.712),进入亚急性期蛋白质含量在400 mg·kg~(-1)时被显著诱导(P=0.039,P0.05);TBBPA对蚯蚓SOD酶活性的影响,在急性试验中50 mg·kg~(-1)时被显著诱导(P0.01),在亚急性试验中400 mg·kg~(-1)时SOD酶活性显著升高(P0.05);TBBPA对蚯蚓GST酶活性影响,在急性试验中400 mg·kg~(-1)时呈现显著诱导效应(P0.05),在亚急性期各处理间差异不显著(P=0.428)。蚯蚓生长抑制率、蛋白质含量、体内各生化酶系对TBBPA暴露的时间效应和剂量效应的敏感性存在不同程度差异,应依据污染暴露指标的有效性和敏感性选择多时间段检测和多指标进行土壤安全评价诊断。
[Abstract]:The growth inhibition rate, protein content, superoxide dismutase (SOD) activity and glutathione transferase (GST) activity of (Eisenia fetida) were investigated in natural soil. The effects of non-lethal dose stress of tetrabromobisphenol A (tetrabromobisphenol A TBBPA) on earthworm growth and antioxidant defense were studied under acute (14 d),) subacute (28 d) exposure. The results showed that there was a significant dose-effect relationship between TBBPA and earthworm growth inhibition in acute test, while in subacute exposure period (400 mg kg~ (-1), TBBPA significantly inhibited the growth of earthworm (P0.05). In the acute test, there was no significant difference among the treatments (P0. 712), but the protein content in the subacute phase was significantly induced (P0. 039) when the protein content entered the subacute phase (P0. 039). The effect of TBBPA on the SOD activity of earthworm was observed. It was significantly induced at 50 mg kg~ (-1) in acute test (P0.01), significantly increased at 400 mg kg~ (-1) in subacute test (P0.05), and significantly induced GST activity in earthworm at 400 mg kg~ (-1) in acute test (P0.05), but there was no significant difference among different treatments in subacute phase (P0.428). The inhibition rate of earthworm growth, the content of protein, the sensitivity of each biochemical enzyme system to TBBPA exposure were different in different degrees. According to the effectiveness and sensitivity of pollution exposure indicators, soil safety assessment and diagnosis should be carried out in different time periods.
【作者单位】：辽宁科技学院生物医药与化学工程学院辽宁省生物医药与化学工程重点实验室;中国科学院生态环境研究中心城市与区域生态国家重点实验室;
【基金】：国家自然科学基金资助项目(No.41671483) 国家国际科技合作专项(No.2012DFA91150)
【分类号】：X171.5

【相似文献】