锑胁迫下丛枝菌根真菌对玉米生长与锑吸收及抗氧化酶的影响

发布时间：2019-04-09 12:18

【摘要】：为了探讨AM(arbuscular mycorrhizal,丛枝菌根)真菌在Sb(锑)胁迫下对农作物生长及吸收Sb的影响,采用盆栽试验研究了在不同Sb添加量[即w(Sb)分别为0、500、1 000 mg/kg]下,接种AM真菌对玉米植株生物量以及N、P和Sb的吸收、膜脂过氧化[MDA(丙二醛)]和抗氧化酶活性的影响.结果表明:随着土壤中Sb添加量的增加,玉米植株的生物量、w(TN)和w(TP)均呈显著下降趋势,植株体内的w(Sb)和Sb积累量、MDA含量、CAT(过氧化氢酶)以及POD(过氧化酶)活性均显著上升.与未接种组相比,接种AM真菌显著促进了玉米植株的生长,提高了玉米植株地上部分的w(TN)和w(TP).在3个Sb添加量处理下,接种显著增加了玉米植株地下部分的w(Sb)、地上和地下部分的Sb积累量以及地上部分的CAT活性,增幅分别为8.90%~23.30%、18.87%~28.37%、27.68%~78.95%及14.92%~88.52%;同时,接种降低了玉米植株中Sb的转运率、玉米植株地上部分的w(Sb)和MDA含量,在1000 mg/kg Sb添加量下差异达显著水平,三者分别降低了36.35%、22.81%和24.29%.研究显示,在Sb污染环境下,接种AM真菌能够减轻玉米植株膜脂过氧化程度,在提高玉米植株地下部分w(Sb)的同时,也会降低Sb向地上部分的转运,减轻Sb对玉米的毒害作用.
[Abstract]:In order to investigate the effects of arbuscular mycorrhizal fungi (AM (arbuscular mycorrhizal,) on the growth and Sb uptake of crops under Sb (antimony) stress, pot experiments were conducted to study the effects of different amounts of Sb (w (Sb) = 0, 500, 1 000 mg/kg, respectively) on the growth and uptake of Sb in crops. The effects of inoculating AM fungi on biomass, uptake of N, P and Sb, membrane lipid peroxidation [MDA (malondialdehyde)] and antioxidant enzyme activities in maize plants were studied. The results showed that with the increase of Sb content in soil, the biomass, w (TN) and w (TP) of maize decreased significantly, and the accumulation of w (Sb) and Sb and the content of MDA in the plant decreased significantly. The activities of CAT (catalase) and POD (peroxidase) were significantly increased. Compared with the non-inoculated group, inoculation of AM fungi significantly promoted the growth of maize plants, and increased the w (TN) and w (TP). In the aboveground parts of maize plants. Under the treatment of three Sb treatments, the Sb accumulation above ground and underground part of w (Sb), and the CAT activity of above ground part were significantly increased by 8.90% and 23.30%, respectively, in the subterranean part of maize plant, and the inoculation increased significantly by 8.90% and 23.30% respectively in the subsurface part of maize plant. 18.87%, 28.37%, 27.68%, 78.95% and 14.92%, 88.52%, respectively. At the same time, inoculation decreased the transfer rate of Sb in maize plants, and the contents of w (Sb) and MDA in the aboveground parts of maize plants were significantly different at 1000 mg/kg Sb, which decreased by 36.35%, 22.81% and 24.29%, respectively. The results showed that inoculation of AM fungi could reduce the degree of membrane lipid peroxidation in maize plants under the environment of Sb pollution, and could not only increase the w (Sb) of underground part of maize plants, but also decrease the transport of Sb to the aboveground parts of maize plants. Reduce the toxic effect of Sb on maize.
【作者单位】：湖南农业大学资源环境学院;中国环境科学研究院环境基准与风险评估国家重点实验室;
【基金】：国家自然科学基金项目(41271338,41303066) 科技部科研院所技术开发研究专项(2014EG166135)
【分类号】：X172;X53

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