水华蓝藻及其毒素降解菌的降解能力和机理研究
发布时间：2021-07-08 07:05
随着淡水水体富营养化程度的加剧,蓝藻水华（Cyanobacterial blooms）在全球范围的爆发日趋严重,对水生生态,经济,甚至是人类的健康都可能产生不良影响,引发了严重的环境问题。目前,已经有大量关于蓝藻水华控制和蓝藻毒素降解的相关研究。其中溶藻微生物（溶藻细菌）已经被作为一种可以抑制蓝藻水华和降解蓝藻毒素的方法而得到广泛研究。然而,在溶藻微生物的相关研究中,除了对一种可产生抗菌素真菌的抑藻能力的研究外,对于溶藻真菌的情况还知之甚少。与此同时,尽管溶藻细菌有一定的抑制藻类生长的能力,但是相比于真菌而言,随着溶藻细菌自身的繁殖,细胞数量的增加会大大降低水体的透明度,且溶藻细菌的次生代谢产物多,变异快,不易控制等缺点都会影响到溶藻细菌的实际应用。自2007年以来,我国太湖也爆发了大规模的蓝藻水华,对当地的人民生活,经济及社会的发展都造成了严重的影响。为了能够更好的控制蓝藻水华,减小其造成的危害,本研究分离到两株白腐真菌Trichaptum abietinum 1302BG和Lopharia spadicea,并对其抑藻能力进行了研究。1.本论文研究了两株白腐真菌对不同淡水蓝藻的抑制...
【文章来源】：南京大学江苏省 211工程院校 985工程院校 教育部直属院校
【文章页数】：131 页
【学位级别】：博士
【文章目录】：
LIST oF ABBREVIATIONS
摘要
ABSTRACT
CHAPTER 1:INTRODUCTION
1. CYANOBACTERIA
2. HARMFULALGAL BLOOMS(HABs)
2.1 Harmful cyanobacteria
2.2 Harmful cyanobacterial blooms
3. RESEARCH OBJECTIVES
REFERENCES
CHAPTER 2:SCREENING FUNGAL STRAINS FROM BAMBOO SOILS AND EVALUATE THEIR ALGAE INHIBITION CAPACITIES AND MECHANISMS
1. INTRODUCTION
2. MATERIALS AND METHODS
2.1. Cyanobacterial strains and cultivation
2.2. Fungal isolation,identification,culture and maintenance
2.3. Degradation test of TA-1302 on algae
2.4. Lipid peroxidation
2.5. Biomass of mycelial pellicle
2.6. Algae lysis
2.7. Scanning electron microscopy (SEM)
2.8. Transmission electron microscopy (TEM)
2.9. Statistical analysis
3. Results
3.1. Changes in algal cells during incubation
3.2. Changes in lipid peroxidation
3.3. The mass of mycelial pellicle
3.4. Degradation mechanism of the algal cells
3.5 Identification and phylogenetic analysis of TA-1302
4. Discussion
References
CHAPTER 3: A CAPABLE OF TRICHAPTUM ABIETINUM 1302BG ONDEGRADING MICROCYSTIN-LR AND REDUCING BIOMASS IN THEALGAL CULTURE
1. Introduction
2. Materials and methods
2.1. Phytoplankton strains and cultivation
2.2 Fungal isolation, culture, and maintenance
2.3 Degradation test of TA-1302 on algae and MC-LR
2.4 HPLC analysis
2.5 Antioxidant enzyme activity assays
2.6 Toxicity studies
2.7 Statistical analysis
3. Results
3.1 The biomass of algal cultures
3.2 Changes in concentration of MC-LR
3.3 Effects of MC-LR on antioxidant enzymes
3.4 The toxicity of degraded algal cuture
4. Discussion
References
CHAPTER 4: EVALUATION OF ALGICIDAL ABILITY WITH FUNGI ONDIFFERENT FRESHWATER ALGAE SPECIES
1. Introduction
2. Materials and methods
2.1. Algal strains and cultivation
2.2. Fungal strains and maintenance
2.3. Inhibiting tests of different fungi on different algal species
2.4. Dry weight of mycelial pellicle
2.5. Statistical analysis
3. Results
3.1 Preliminary screening of algicidal fungi
3.2 Variations in biomass of algicidal fungi during incubation
4. Discussion
References
CHAPTER 5: A NEW STRATEGY FOR DEALING WITH THECYANOBACTERIAL BLOOMS
1. Introduction
2. Materials and methods
2.1 Site and sampling
2.2 Fungal culture and maintenance
2.3 Growth test
2.4 Degradation test of TA-1302 and LS-1302 on cyanobacterial bloom algae
2.5 Statistical analysis
3. Results
3.1 Mycelial plaque of TA-1302 andLS-1302
3.2 Dry weight of TA-1302 andLS-1302
3.3 Changes in biomass of culture during incubation
4. Discussion
References
CHAPTER 6: SUMMARY
6.1 Conclusions
6.2 Main innovation
6.3 Prospects
SUPPLEMENTAL MATERIAL
PRODUCTIONS
ACKNOWLEDGEMENTS
附件
【参考文献】：
期刊论文
[1]Isolation and algae-lysing characteristics of the algicidal bacterium B5[J]. MU Rui-min FAN Zheng-qiu PEI Hai-yan YUAN Xue-liang Liu Si-xiu WANG Xiang-rong 1.Department of Environmental Science and Engineering,Fudan University,Shanghai 200433,China. 2.College of Environmental Science and Engineering,Ocean University of China,Qingdao 266003,China 3.Environmental Research Institute,Shandong University,Jinan 250100,China. Journal of Environmental Sciences. 2007(11)
[2]太湖微囊藻毒素年变化及其与浮游生物的关系[J]. 许秋瑾,高光,陈伟民. 中国环境科学. 2005(01)
[3]APPLICATION OF CLAYS TO REMOVAL OF RED TIDE ORGANISMS Ⅱ.COAGULATION OF DIFFERENT SPECIES OF RED TIDE ORGANISMS WITH MONTMORILLONITE AND EFFECT OF CLAY PRETREATMENT[J]. 俞志明,邹景忠,马锡年. Chinese Journal of Oceanology and Limnology. 1994(04)
本文编号：3271102
【文章来源】：南京大学江苏省 211工程院校 985工程院校 教育部直属院校
【文章页数】：131 页
【学位级别】：博士
【文章目录】：
LIST oF ABBREVIATIONS
摘要
ABSTRACT
CHAPTER 1:INTRODUCTION
1. CYANOBACTERIA
2. HARMFULALGAL BLOOMS(HABs)
2.1 Harmful cyanobacteria
2.2 Harmful cyanobacterial blooms
3. RESEARCH OBJECTIVES
REFERENCES
CHAPTER 2:SCREENING FUNGAL STRAINS FROM BAMBOO SOILS AND EVALUATE THEIR ALGAE INHIBITION CAPACITIES AND MECHANISMS
1. INTRODUCTION
2. MATERIALS AND METHODS
2.1. Cyanobacterial strains and cultivation
2.2. Fungal isolation,identification,culture and maintenance
2.3. Degradation test of TA-1302 on algae
2.4. Lipid peroxidation
2.5. Biomass of mycelial pellicle
2.6. Algae lysis
2.7. Scanning electron microscopy (SEM)
2.8. Transmission electron microscopy (TEM)
2.9. Statistical analysis
3. Results
3.1. Changes in algal cells during incubation
3.2. Changes in lipid peroxidation
3.3. The mass of mycelial pellicle
3.4. Degradation mechanism of the algal cells
3.5 Identification and phylogenetic analysis of TA-1302
4. Discussion
References
CHAPTER 3: A CAPABLE OF TRICHAPTUM ABIETINUM 1302BG ONDEGRADING MICROCYSTIN-LR AND REDUCING BIOMASS IN THEALGAL CULTURE
1. Introduction
2. Materials and methods
2.1. Phytoplankton strains and cultivation
2.2 Fungal isolation, culture, and maintenance
2.3 Degradation test of TA-1302 on algae and MC-LR
2.4 HPLC analysis
2.5 Antioxidant enzyme activity assays
2.6 Toxicity studies
2.7 Statistical analysis
3. Results
3.1 The biomass of algal cultures
3.2 Changes in concentration of MC-LR
3.3 Effects of MC-LR on antioxidant enzymes
3.4 The toxicity of degraded algal cuture
4. Discussion
References
CHAPTER 4: EVALUATION OF ALGICIDAL ABILITY WITH FUNGI ONDIFFERENT FRESHWATER ALGAE SPECIES
1. Introduction
2. Materials and methods
2.1. Algal strains and cultivation
2.2. Fungal strains and maintenance
2.3. Inhibiting tests of different fungi on different algal species
2.4. Dry weight of mycelial pellicle
2.5. Statistical analysis
3. Results
3.1 Preliminary screening of algicidal fungi
3.2 Variations in biomass of algicidal fungi during incubation
4. Discussion
References
CHAPTER 5: A NEW STRATEGY FOR DEALING WITH THECYANOBACTERIAL BLOOMS
1. Introduction
2. Materials and methods
2.1 Site and sampling
2.2 Fungal culture and maintenance
2.3 Growth test
2.4 Degradation test of TA-1302 and LS-1302 on cyanobacterial bloom algae
2.5 Statistical analysis
3. Results
3.1 Mycelial plaque of TA-1302 andLS-1302
3.2 Dry weight of TA-1302 andLS-1302
3.3 Changes in biomass of culture during incubation
4. Discussion
References
CHAPTER 6: SUMMARY
6.1 Conclusions
6.2 Main innovation
6.3 Prospects
SUPPLEMENTAL MATERIAL
PRODUCTIONS
ACKNOWLEDGEMENTS
附件
【参考文献】：
期刊论文
[1]Isolation and algae-lysing characteristics of the algicidal bacterium B5[J]. MU Rui-min FAN Zheng-qiu PEI Hai-yan YUAN Xue-liang Liu Si-xiu WANG Xiang-rong 1.Department of Environmental Science and Engineering,Fudan University,Shanghai 200433,China. 2.College of Environmental Science and Engineering,Ocean University of China,Qingdao 266003,China 3.Environmental Research Institute,Shandong University,Jinan 250100,China. Journal of Environmental Sciences. 2007(11)
[2]太湖微囊藻毒素年变化及其与浮游生物的关系[J]. 许秋瑾,高光,陈伟民. 中国环境科学. 2005(01)
[3]APPLICATION OF CLAYS TO REMOVAL OF RED TIDE ORGANISMS Ⅱ.COAGULATION OF DIFFERENT SPECIES OF RED TIDE ORGANISMS WITH MONTMORILLONITE AND EFFECT OF CLAY PRETREATMENT[J]. 俞志明,邹景忠,马锡年. Chinese Journal of Oceanology and Limnology. 1994(04)
本文编号：3271102
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