脱落酸对大鼠学习记忆的影响及可能机理

发布时间：2018-01-06 03:32

本文关键词：脱落酸对大鼠学习记忆的影响及可能机理　出处：《合肥工业大学》2017年硕士论文　论文类型：学位论文

更多相关文章： ABA 树突棘 树突棘密度 NDR1/2激酶

【摘要】：脱落酸(ABA)作为关键的植物激素,广泛存在于植物体内,现研究发现ABA也存在于动物(包括人)组织中,且具有多种生物活性。ABA作为类胡萝卜素的直接衍生物,和视黄酸(RA)具有相似的分子结构,研究表明RA可以明显改善啮齿动物的空间记忆能力,但ABA对空间学习与记忆的影响及其分子机制尚不明确。目的:探究ABA给药对成年SD大鼠空间记忆的影响;探究ABA给药对SD大鼠海马树突棘密度及形态的影响,并从NMDA受体、Arc蛋白以及NDR1/2激酶途径来探究其可能的分子机制。方法:1.ABA给药实验:ABA给药剂量分别为5 mg/kg/day、25 mg/kg/day、50 mg/kg/day,给药方式为腹腔注射,给药时间为SD大鼠出生第七天开始一直到第八周进行水迷宫实验的前一天为止;2.Golgi-cox染色:观察ABA给药后海马神经细胞的树突棘形态,通过Western blot及荧光定量PCR检测NMDA受体、Arc蛋白以及NDR1/2激酶的蛋白表达。结果:1.行为学测试发现,中剂量ABA增加了大鼠在目标象限中花费的持续时间和在Morris水迷宫(MWM)的探测测试中进入的频率,并减少了于目标象限的等待时间。2.ABA能引起树突棘结构的显著变化,表现为增加树突棘的形态复杂性、树突棘密度和成熟蘑菇状树突棘数目的增加。3.此外,ABA对NMDA受体R1、R2及Arc蛋白的表达无显著影响,进一步研究发现,ABA能显著提高NDR1/2蛋白及下游基因Rabin3的表达量;离体的原代海马神经元转染NDR1/2 shRNA,研究发现NDR下游基因Rabin3表达的显著降低,即进一步验证了NDR1/2激酶途径参与了ABA对大鼠海马区神经元成熟过程的调节。结论:ABA通过NDR1/2激酶途径调节大鼠海马区神经元树突棘成熟过程,从而增强SD大鼠的学习记忆能力。
[Abstract]:Abscisic acid abscisic acid (Aba), as a key plant hormone, widely exists in plants. Now it has been found that ABA also exists in animal (including human) tissues. ABA as a direct derivative of carotenoid and retinoic acid (RAA) has similar molecular structure. Studies show that RA can significantly improve the spatial memory ability of rodents. But the effect of ABA on spatial learning and memory and its molecular mechanism are not clear. Objective: to explore the effect of ABA administration on spatial memory in adult SD rats. To investigate the effect of ABA administration on the density and morphology of hippocampal dendritic spine in SD rats, and to investigate the effects of NMDA receptor. Arc protein and NDR1/2 kinase pathway were used to explore its possible molecular mechanism. Methods: 1. The dosages of Arc protein and NDR1/2 kinase were 5 mg/kg/day respectively. 25 mg / kg / kg / day 50 mg / kg / kg / day, administered by intraperitoneal injection. The time of administration was from 7th days after birth to the day before the water maze test at 8th weeks. 2. Golgi-cox staining: the morphology of dendritic spine of hippocampal neurons was observed after ABA administration, and NMDA receptor was detected by Western blot and fluorescence quantitative PCR. Arc protein and NDR1/2 kinase protein expression. Results: 1. Behavioral test found. Medium-dose ABA increased the duration spent in the target quadrant and the frequency of entry in the Morris water maze (MWM) detection test. And reduced the waiting time in the target quadrant. 2. ABA can cause significant changes in the structure of dendritic spine, as shown by increasing the morphological complexity of dendritic spine. The density of dendritic spine and the number of mature mushroom dendritic spine increased .3.In addition, there was no significant effect of NMDA on the expression of R1R ~ 2 and Arc protein. ABA could significantly increase the expression of NDR1/2 protein and downstream gene Rabin3. The primary hippocampal neurons were transfected with NDR1/2 shRNAs in vitro. It was found that the Rabin3 expression of NDR downstream gene was significantly decreased. It is further verified that the NDR1/2 kinase pathway is involved in the regulation of ABA on the maturation of hippocampal neurons in rats. Conclusion:. ABA regulates the maturation of dendritic spine in rat hippocampal neurons through NDR1/2 kinase pathway. So as to enhance the learning and memory ability of SD rats.
【学位授予单位】：合肥工业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：Q42

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