煤炭地下气化废水中苯酚的微生物降解研究

发布时间：2018-01-07 12:14

  本文关键词：煤炭地下气化废水中苯酚的微生物降解研究　出处：《河南理工大学》2015年硕士论文　论文类型：学位论文

  更多相关文章： 煤炭地下气化 地下水污染 苯酚 细菌 降解

【摘要】：煤炭地下气化直接在地下将煤炭转化成气体燃料或原料,被誉为“绿色”开采技术,但气化过程中产生的有机污染物可能对地下水造成污染,影响其推广应用。有机污染物中尤以酚类化合物含量最高,主要为苯酚、甲酚、二甲酚等。酚类化合物为原生质毒物,对所有生物均会产生一定毒害作用,我国把苯酚列为中国环境优先控制污染物,因此对酚类化合物的脱除应是煤炭地下气化地下水有机污染物去除的重点。本文以活性污泥为菌源,以苯酚为唯一碳源筛选得到两株苯酚优势降解菌:JC和WX,经生理生化试验和16SrDNA鉴定,JC为绿脓假单胞菌,WX为无色杆菌。考察发现JC与WX菌最适生长环境为:温度30~40℃,pH 7,摇床转速120r/min,接种量7~16%。在苯酚浓度800mg/L,摇床转速120r/min,温度40℃的环境中,40~48h内细菌生长繁殖最快,细菌活性也最高。分别利用管式炉及自行搭建的地下气化模拟系统,以褐煤为原料进行水蒸气气化模拟试验,收集煤气洗涤水。用JC及WX菌对管式炉煤气洗涤水原水TOC浓度为570mg/L及稀释后TOC分别为375.07mg/L、211.94mg/L的废水进行降解处理,均具有良好降解效果,TOC为211.94mg/L的废水经60h降解处理后,TOC含量分别下降到64.427mg/L、62.944mg/L,TOC降解率分别为69.6%、70.3%。对煤炭地下气化模拟废水,将TOC从1089.19 mg/L稀释到175.07 mg/L,经JC及WX菌处理96h,废水中TOC分别降低到42.01 mg/L、47.44 mg/L;使用美国安捷伦气相色谱与质谱联用仪(HP 7890/5975)测定废水中有机污染物组分及含量,经细菌降解后废水有机污染物中含量最高的苯酚、邻甲苯酚、间甲苯酚含量为0,其他酚类化合物含量明显降低,降解后废水中出现烷烃类、烯烃类等小分子链烃化合物,可能细菌对苯酚等有机物的降解是逐步进行的,先将大分子物质分解为小分子,再进行小分子有机物的降解。
[Abstract]:Underground gasification of coal directly converts coal into gas fuel or raw material, which is called "green" mining technology, but organic pollutants produced in gasification process may cause pollution to groundwater. The organic pollutants are mainly phenol, cresol, dimethylphenol and so on. Phenolic compounds are protoplast poisons, which have a certain toxic effect on all organisms. In China, phenol is listed as a priority pollutant for environmental control in China, so the removal of phenolic compounds should be the focus of removal of organic pollutants from underground coal gasification groundwater. In this paper, activated sludge is used as the source of bacteria. Using phenol as the sole carbon source, two strains of phenol-degrading bacteria were isolated and identified as Pseudomonas aeruginosa by physiological and biochemical tests and 16s rDNA. The optimum growth environment of JC and WX was as follows: temperature 30 ~ 40 鈩，

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