miRNA和高脂食物对达乌尔黄鼠糖脂代谢的影响

发布时间：2018-01-10 20:04

本文关键词：miRNA和高脂食物对达乌尔黄鼠糖脂代谢的影响　出处：《沈阳师范大学》2017年硕士论文　论文类型：学位论文

【摘要】：为了探究miRNA及高脂食物对贮脂类冬眠动物达乌尔黄鼠(Spermophilus dauricus)从冬眠前育肥期至冬眠期这一过程中对能量选择发生的调节机制及其适应性变化的影响,采用第二代转录组测序(RNA-Seq)技术,对达乌尔黄鼠起始育肥期、快速育肥期、育肥完成期和冬眠期四个阶段的白色脂肪组织进行了测序,并分析了与代谢相关的miRNA差异表达情况。结果表明:在脂肪酸合成通路中,rno-miR-30a-3p、rno-miR-30e-3p在育肥完成期显著上调,rno-miR-106b-3p在育肥完成期显著上调。此时达乌尔黄鼠的体重呈下降趋势,提示在此期间这些miRNA可能抑制脂肪酸合成;在糖酵解/糖异生通路中,rno-miR-139-5p、rno-mi R-185-5p、rno-miR-206-3p、rno-miR-23b-5p、rno-miR-29b-2-5p在快速育肥期和育肥完成期下调,rno-miR-30e-3p和rno-miR-135a-3p、rno-194-5p在快速育肥期下调,rno-miR-32-5p在完成育肥期下调,rno-miR-130b-3p、rno-miR-144-5p、rno-mi R-194-5p、rno-miR-196b-5p、rno-miR-449a-5p、rno-miR-451-5p在冬眠期上调;在TCA循环通路中,rno-miR-181a-5p、rno-miR-350、rno-mi R-7a-5p在冬眠期上调。从快速育肥期到完成育肥期,黄鼠体重快速增加到顶峰的过程,黄鼠的供能主要来自食物,以糖代谢为主。进入冬眠期,糖代谢降低,推测此时已转化为脂代谢供能为主。为了探究高脂食物对达乌尔黄鼠糖脂代谢和冬眠的影响,通过对快速育肥期的达乌尔黄鼠喂以高脂食物,在饲喂前后对呼吸商和血糖稳态进行测定,在饲喂期间对体重和能量收支进行测定,在饲喂之后的冬眠期进行冬眠阵的观察。结果表明:1.在饲喂高脂食物的第60天高脂组体重显著高于对照组,高脂组在第48天和第54天的消化率和可代谢能率高于对照组,推测高脂食物可能通过增加消化率和可代谢能效率增加体重,推迟完成育肥期的到来;2.高脂食物饲喂后的高脂组呼吸商显著低于饲喂前,提示饲喂后高脂组代谢底物转变为以脂脂肪为主;3.高脂组血糖耐受性显著低于对照组,胰岛素敏感性没有差异,推测高脂食物可能会导致;4.高脂食物饲喂一个月对冬眠情况可能并没有影响。
[Abstract]:In order to explore the miRNA and high fat diet on lipid storage in the hibernating animal ground squirrel (Spermophilus dauricus) the choice of the regulating mechanism and adaptive changes influence on the energy from the front to the fattening period of hibernation hibernation this process, using the second generation transcriptome sequencing (RNA-Seq) technology of Citellus dauricus starting fast fattening period. The fattening period, the four stages of fattening period and complete hibernation of white adipose tissue was sequenced, and analyzed the difference of miRNA expression correlated with metabolism. The results showed that rno-miR-30a-3p in fatty acid synthesis pathway, and rno-miR-30e-3p was significantly up-regulated in fattening period, rno-miR-106b-3p period was significantly up-regulated in fattening. At this time of Citellus dauricus the body weight decreased, suggesting that during these miRNA may inhibit fatty acid synthesis; in glycolysis / gluconeogenesis pathway, rno-miR-139-5p, rno-mi, R-185-5p, R No-miR-206-3p, rno-miR-23b-5p, rno-miR-29b-2-5p in the fast fattening period and fattening period complete cut, rno-miR-30e-3p and rno-miR-135a-3p, rno-194-5p in the fast fattening period down, rno-miR-32-5p finished the fattening period down, rno-miR-130b-3p, rno-miR-144-5p, rno-mi, R-194-5p, rno-miR-196b-5p, rno-miR-449a-5p, rno-miR-451-5p in the hibernation period increases; in the TCA pathway, rno-miR-181a-5p, rno-miR-350, rno-mi and R-7a-5p in hibernation during the period of rise. From the fast fattening period to complete the fattening period, body weight increased rapidly to the peak of the ground squirrel, squirrel supply mainly from food to sugar metabolism. Into hibernation, reduced glucose metabolism, lipid metabolism has been transformed into speculation at this time can be based. In order to explore the effects of high fat diet on glucose and lipid metabolism in ground squirrels and through the rapid ur hibernation, fattening period of Citellus dauricus fed with high fat food, feed and The respiratory quotient and glucose homeostasis were measured in the feeding period on body weight and energy balance were measured after feeding in hibernation hibernation were observed. The results showed that 1. in sixtieth high fat group fed high fat food weight was significantly higher than the control group, high fat group in forty-eighth days and fifty-fourth days of digestion and the rate of metabolism was higher than the control group, high fat foods that could increase digestibility and metabolic efficiency can increase weight, delay the arrival of the fattening period; high fat group respiratory quotient 2. high fat diet after feeding was significantly lower than that before feeding, feeding after high fat group metabolic substrate changed to fatty. The high fat group 3.; glucose tolerance was significantly lower than the control group, there was no difference in insulin sensitivity, high fat food may lead to speculation; 4. fed a high-fat diet for one month may hibernate does not influence.

【学位授予单位】：沈阳师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：Q41

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