荷叶多酚的提

发布时间：2018-03-19 17:45

  本文选题：荷叶　切入点：多酚　出处：《安徽工程大学》2017年硕士论文　论文类型：学位论文

【摘要】：荷叶为睡莲科植物莲的叶片。在我国,其种植范围面积广,资源十分丰富。目前,国内对荷叶的开发利用主要在粗加工方面,导致荷叶利用程度较低。荷叶中富含多酚类物质,它具有抗氧化、抑菌、减肥降脂、抗辐射、增强机体免疫性、抗炎抗病毒、抗衰老等多种功能,具有较大的应用价值。本文选取荷叶作为实验原材料,研究荷叶中多酚的提取条件,通过体外抗氧化实验考察其抗氧化能力,初步研究了荷叶多酚微胶囊的制备条件,开发了一种富含荷叶多酚的功能性黄酒的制备方法,并研究其理化指标。本文的主要研究结果如下:1、荷叶粗多酚的提取条件优化以荷叶为原材料,在单因素实验的基础上,经过响应面实验优化,确定荷叶中粗多酚的最佳提取条件。研究结果表明,荷叶粗多酚的最佳提取工艺条件为:液料比70 mL/g、浸提温度47 ℃C、浸提时间97min,在此条件下荷叶粗多酚的提取率为54.12 mg/g。2、荷叶粗多酚的抗氧化活性研究以荷叶粗多酚为研究对象,考察其对羟基自由基、DPPH自由基、ABTS自由基的清除效果。研究结果表明,在实验浓度范围内,荷叶粗多酚对羟基自由基、DPPH自由基、ABTS自由基的清除能力随着浓度的增大而增强,具有明显的浓度依赖性,但在同等浓度下,荷叶粗多酚对自由基的清除效果低于对照组VC的清除效果。3、荷叶多酚微胶囊的制备及释放性研究以荷叶多酚为芯材,壳聚糖、海藻酸钠为壁材,制备荷叶多酚微胶囊,并通过扫描电镜(SEM)和傅里叶变换光谱(FT-IR)对其表征,同时对荷叶多酚微胶囊进行体外释放研究。实验结果表明,荷叶多酚成功包覆于微胶囊内,且包埋率达到了 70.04%。体外释放研究表明,荷叶多酚微胶囊能较好的达到缓释效果。4、荷叶多酚黄酒的制备在传统淋饭法酿造黄酒的基础上,通过添加荷叶多酚制备荷叶多酚功能性黄酒。分析比较荷叶多酚的添加时间、添加量对黄酒理化指标的影响,确定最优酿造条件为:在黄酒后发酵过程中添加荷叶多酚,其添加量为300 mg/L,所酿造的黄酒富含荷叶多酚功能性成份。成品黄酒中:酒精度:12.2%vol;总糖:15.10 g/L;总酸:6.67 g/L;氨基酸态氮:0.42 g/L;多酚含量:0.453 g/L;氨基酸含量为3.64 g/L。荷叶多酚黄酒样品中检测出主要挥发性成份有58种,检测出的挥发性成分的种类比对照组黄酒样品增加了 18种。
[Abstract]:The lotus leaf is the leaf of the water lily plant. In our country, its planting area is wide and the resources are very rich. At present, the exploitation and utilization of lotus leaf in our country is mainly in rough processing, which leads to the low utilization degree of lotus leaf, and the lotus leaf is rich in polyphenols. It has many functions, such as antioxidation, bacteriostasis, weight loss and lipid-lowering, anti-radiation, enhancing body immunity, anti-inflammation and anti-virus, anti-aging and so on. The extraction conditions of polyphenols from lotus leaves were studied. The antioxidant capacity of polyphenols in lotus leaves was investigated in vitro. The preparation conditions of lotus leaf polyphenols microcapsules were preliminarily studied, and a preparation method of functional yellow rice wine rich in lotus leaf polyphenols was developed. The main results of this paper are as follows: 1. The extraction conditions of lotus leaf polyphenols were optimized using lotus leaves as raw materials. On the basis of single factor experiments, the response surface experiments were carried out to optimize the extraction conditions. The optimum extraction conditions of crude polyphenols from lotus leaves were determined. The optimum extraction conditions were as follows: the ratio of liquid to material was 70 mL / g, the extraction temperature was 47 鈩，

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