不同皮制来源胶原多肽对小鼠老化皮肤作用的研究

发布时间：2018-03-19 06:45

本文选题：胶原多肽　切入点：抗氧化　出处：《江南大学》2017年硕士论文　论文类型：学位论文

【摘要】：本研究根据消费市场上胶原多肽的通行相对分子量,采用酶法制备了猪皮、鸡皮、鱼皮胶原多肽。通过皮下注射D-半乳糖制备了小鼠皮肤衰老模型,同时给予不同组小鼠灌胃胶原多肽,分别为空白组、模型组、鱼皮高剂量组、鱼皮低剂量组,猪皮高剂量组、猪皮低剂量组、鸡皮高剂量组、鸡皮低剂量组以及氨基酸组。饲养结束后,对小鼠的血液生化指标、皮肤生化指标、皮肤特征性RNA等进行了分析。结果表明:1.D-半乳糖衰老模型能使小鼠皮肤变薄,变松弛,使皮肤胶原纤维减少。而三种皮制来源的胶原多肽都能对延缓皮肤变薄,增加胶原纤维含量具有一定作用。2.D-半乳糖至衰老模型可使小鼠皮肤中羟脯氨酸含量下降,SOD活力下降,MDA值上升,GSH活力下降,MMPs活力上升。而服用胶原多肽后,相比于D半乳糖模型对照组,猪皮胶原多肽组皮肤中羟脯氨酸提高了25.12%,SOD活力提高了83.49%,MDA值减少27.94%,GSH活力提高了25.39%;鱼皮胶原多肽组羟脯氨酸含量提高了32.93%,SOD活力提高了70.09%,MDA含量减少32.13%,GSH活力提高了24.24%;鸡皮胶原多肽组羟脯氨酸提高27.76%,SOD活力提高了47.67%,MDA含量减少23.89%,GSH活力提高了14.85%。3.通过制备小鼠皮肤基因芯片,并进行分析验证。结果显示:有关小鼠皮肤衰老的基因大约有2763个。衰老模型中中下调基因1488个,上调基因1275个。再通过聚类分析、基因富集分析、KEGG通路分析、GO分析以及半定量PCR检测的结果表明了影响不同皮制来源胶原多肽代谢的主要途径:既能通过上调脯氨酸羟化酶的表达影响胶原的合成,又能通过下调基质金属蛋白酶的表达,提高基质金属蛋白酶与其抑制剂相对表达量的比值,影响胶原的降解。结论:不同皮制来源的胶原多肽都能够延缓D半乳糖致衰老的小鼠皮肤老化,提高机体的抗氧化能力,调节胶原合成和降解途径中相关基因的表达,并且猪皮胶原多肽的效果最好,鱼皮胶原多肽次之,而鸡皮胶原多肽则相对较差。
[Abstract]:According to the relative molecular weight of collagen peptides in the consumer market, the collagen peptides of pig skin, chicken skin and fish skin were prepared by enzymatic method, and the skin aging model of mice was established by subcutaneous injection of D-galactose. At the same time, different groups of mice were given intragastric administration of collagen polypeptide, which were blank group, model group, fish skin high dose group, fish skin low dose group, pig skin high dose group, pig skin low dose group, chicken skin high dose group. After feeding, the blood biochemical index, skin biochemical index, skin characteristic RNA and so on of mice were analyzed. The results showed that 1. 1. D-galactose aging model could make skin thin and relax in mice. Reduce collagen fibers in the skin. And collagen peptides from all three skin sources can slow skin thinning. Increasing the content of collagen fibers has a certain effect. 2. D-galactose to aging model can reduce the content of hydroxyproline in skin of mice and decrease the activity of SOD. The activity of GSH increases and the activity of MMPs increases. However, after taking collagen polypeptide, the activity of MMPs is increased. Compared with D-galactose model control group, The content of hydroxyproline in the skin of collagen polypeptide group increased the activity of SOD, the activity of SOD increased by 83.49 and the MDA value decreased by 27.94 and GSH increased 25.39; the content of hydroxyproline in collagen polypeptide group increased the activity of SOD and the activity of SOD increased by 70.09; the activity of GSH increased by 24.244in the skin of chicken skin; The activity of SOD in collagen polypeptide group increased by 27.760.MDA content decreased by 23.89% and GSH activity increased by 14.85.3.Through the preparation of mouse skin gene chip, the activity of GSH was increased. The results showed that there were about 2763 genes related to skin senescence in mice. In the aging model, 1488 genes were down-regulated and 1275 genes were up-regulated. The results of KEGG pathway analysis and semi-quantitative PCR analysis showed that the main pathway affecting the metabolism of collagen peptides from different skin sources was that the synthesis of collagen could be affected by up-regulating the expression of proline hydroxylase. By down-regulating the expression of matrix metalloproteinase, the ratio of matrix metalloproteinase to its inhibitor can be increased. Conclusion: collagen peptides from different skin systems can delay the aging of aging mice induced by D-galactose, improve the antioxidant ability of the body, and regulate the expression of related genes in collagen synthesis and degradation pathway. The effect of collagen polypeptide in pig skin was the best, followed by collagen polypeptide in fish skin and collagen peptide in chicken skin.
【学位授予单位】：江南大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TS201.2

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