微生物絮凝剂产生菌的廉价培养基研究及应用

发布时间：2018-04-15 16:07

  本文选题：微生物絮凝剂 + 廉价培养　； 参考：《中北大学》2017年硕士论文

【摘要】：本课题选取第三类絮凝剂作为主要对象,通过筛选菌株,进行替代的廉价培养减少成本,并探讨其絮凝剂结构组成与作用机理针对不同的废水研究替代传统絮凝剂,以期提高絮凝效率及范围,废物利用来进行绿色工艺,主要内容与所得结论如下:以生活污水和污泥池筛选,通过富集分化分离出56株菌,选用高岭土测定反应体系具体数值进行筛选,得到17株具有产絮能力的菌株,根据絮凝活性的大小和絮凝稳定性的测定确定来自污泥中的w-3菌株为以后实验的研究对象,絮凝活性高达63.22%。同时归纳种种形态学特性,利用具体理化测验,初步判定此菌属于气单胞菌属。通过拟合的响应面模型,进行实际的测定及优化,对w-3进行富集培养,用以参照的最佳工艺为:pH 7,温度31℃,培养时间50 h、转速141r/min,絮凝率为74.61%。采用较为廉价的豆腐、乳业乳品、白醋食品加工废水作廉价替代品进行驯化。豆腐废水酸碱度为7,引入K2HPO4 0.05g,31℃,培养箱振荡每分钟140r进行50h,测定值为72.43%。乳业废水培养基在pH 7,添加葡萄糖0.05g,CaCl2 0.05g,温度31℃,培养箱振荡每分钟140r,体系值为71.27%。将P-B筛选与响应面结合建立模型,利用数据分析及实际实验参数确定白醋培养条件,确定培养条件最佳工艺参数为:转速140r/min、培养时间48h、温度32℃、pH 6.88、磷酸氢二钾4.08g/L、氯化铵2.39g/L,絮凝率75.66%。通过选取食品废水代替传统方式优化,实验成本降低的同时达到废物绿色循环目的。通过菌株w-3用白醋废水做培养基制备微生物絮凝剂,并对菌液投加量,酸碱性研究,为废水处理的广谱性提供了参考性,并通过对其代谢进行不同方式提纯检测鉴定,通过分布情况看其性质及活性,确定为胞外生物制剂。用白醋废水做培养基培养生物制剂,对实际造纸废水进行处理,投加菌液4.5ml、离子含量提升6ml,色度减少95.60%、COD测定达41.35%,絮凝值达97.89%,可以发现作为食品加工废水白醋有其实际利用意义,获得实际依据。对实际洗煤废水进行利用,絮凝含量添加4ml、助凝剂添加含量4ml时,分光光度值达96.42%。对实际电镀废水进行处理,菌种引入为2mL,酸碱度为5。模型参考为70.71%,通过验证实验值为72.56%,发现模型可以作为参考,能提供有可信度的参数数据,具有实际研究可行性。对絮凝剂进行提纯后,利用电位变化及官能团的不同,检测分析得出:该絮凝剂为多糖类絮凝剂,蛋白质不起絮凝作用,在结构中可以看出有许多支链,通过吸附位点来进行絮凝作用。主要以吸附架桥为反应方式,从而对体系造成作用。
[Abstract]:The third kind of flocculant was selected as the main object. By screening strains, the cost of the flocculant was reduced, and the structure and mechanism of the flocculant was studied to substitute the traditional flocculant for different wastewater.The main contents and conclusions are as follows: 56 strains of bacteria were isolated from domestic sewage and sludge tank by enrichment and differentiation.The specific values of kaolin reaction system were selected and 17 strains with flocculation ability were obtained. According to the determination of flocculation activity and flocculation stability, w-3 strain from sludge was selected as the research object.The flocculation activity is as high as 63.22.At the same time, various morphological characteristics were summarized, and the specific physical and chemical tests were used to identify the bacteria as Aeromonas.Through the fitting response surface model, the actual measurement and optimization were carried out, and the enrichment and culture of w-3 were carried out. The optimum conditions for reference were as follows: ph 7, temperature 31 鈩，

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