绿色木霉发酵法制备苹果渣膳食纤维的工艺及其理化特性的研究

发布时间：2018-04-20 16:49

  本文选题：苹果渣 + 绿色木霉　； 参考：《沈阳农业大学》2017年硕士论文

【摘要】：我国是苹果种植大国,产量居世界前列,苹果产品的大量加工导致苹果渣严重浪费,不仅污染环境而且浪费其作为膳食纤维的优质来源。本文以寒富苹果(Hanfu Apple)、国光苹果(Ralls Apple)、嘎啦苹果(Gala Apple)为原料,探讨了微生物发酵法(绿色木霉发酵法)制取苹果渣膳食纤维(Dietary Fiber)的加工工艺及其理化特性,为苹果渣的综合利用提供了理论依据和指导作用,为苹果渣膳食纤维的工业化生产打下试验基础。主要研究结果如下:在绿色木霉发酵制备膳食纤维试验中,单因素试验显示:发酵剂接种量、培养温度、培养时间和pH值对产品的DF含量有较大影响,采用四因素三水平L9(34)正交试验,确定了绿色木霉发酵法制备寒富苹果渣、国光苹果渣和嘎啦苹果渣膳食纤维的最佳工艺条件为:发酵剂接种量12%、培养温度28℃、培养时间56h、pH7.3。优化条件下得到的寒富苹果渣、国光苹果渣和嘎啦苹果渣膳食纤维与原粉相比,TDF、IDF、SDF含量均比原粉有所提高;绿色木霉发酵法制取的苹果渣膳食纤维中的蛋白质含量和水分与原粉比下降了,而灰分与原粉相比却提升了。在苹果渣膳食纤维漂白试验中,单因素试验显示:H2O2溶液浓度、pH值、培养温度、培养时间对绿色木霉发酵法制备寒富苹果渣脱色效果有很大影响采用四因素三水平L9(34)正交试验,确定其最佳工艺条件为:H2O2浓度为10%、漂白温度50℃、漂白时间2h、pH9。在物化特性的试验中,绿色木霉发酵法制取苹果渣膳食纤维产品具有良好的持水力、溶胀性和持油力;在pH2.0的条件下,绿色木霉发酵法制取的苹果渣膳食纤维的产品对Cu~(2+)的最大束缚量较低,而对Pb~(2+)、Cd~(2+)具有良好的最大束缚量。综上所述:经过本文中绿色木霉发酵制备的苹果渣膳食纤维,其质量明显改善,本试验中的发酵工艺简单,成本较低,将苹果加工后所剩余的渣变废为宝,为进一步的大规模工业化生产奠定了坚实的基础。
[Abstract]:China is a big country of apple planting, and its output is among the highest in the world. The processing of apple products leads to a serious waste of apple pomace, which not only pollutes the environment but also wastes it as a good source of dietary fiber. In this paper, the processing technology and physicochemical properties of Dietary fiber from apple dregs by microbial fermentation (Trichoderma viridis fermentation) were studied by using Hanfu Appleo, Ralls Appleus and Gala Applel of Hanfu, Guoguang and Gala Apple. as raw materials. It provides theoretical basis and guidance for the comprehensive utilization of apple dregs and lays the experimental foundation for the industrial production of apple dregs dietary fiber. The main results are as follows: in the experiment of preparing dietary fiber by Trichoderma viride fermentation, the single factor test showed that inoculation amount of starter, culture temperature, culture time and pH value had great influence on DF content of the product. The orthogonal experiment of four factors and three levels (L9 / 34) was used to determine the optimum conditions for the preparation of cold rich apple dregs by Trichoderma viride fermentation. The optimum technological conditions were as follows: inoculation amount of starter 12, culture temperature 28 鈩，

本文编号：1778642