聚氨酯静电纺丝膜的改性及其抗菌性能

发布时间：2018-05-02 09:02

本文选题：聚氨酯静电纺丝膜 + 多壁碳纳米管　；参考：《聊城大学》2017年硕士论文

【摘要】：敷料是用来覆盖伤口的材料,目的是提供有利的环境使伤口愈合。众所周知,伤口容易被各种细菌感染,从而导致伤口恶化、化脓等,因此,用来覆盖伤口的敷料应具有比较好的抗菌性,从而能够用来防止伤口感染,但是目前所使用的大部分敷料不能满足抗菌的要求。本论文以聚氨酯(TPU)静电纺丝膜为基材,在TPU静电纺丝膜上构建了三类抗菌表面,并且研究了改性前后表面的结构、组成以及抗菌性能。具体研究内容如下:第一:在TPU静电纺丝膜上构建单层杀菌表面。利用超声分散法将多壁碳纳米管(MWCNT)固定到TPU静电纺丝膜上,制成了TPU/MWCNT静电纺丝膜。得到的主要结论如下:(1)射线光电子能谱(XPS)、扫描电子显微镜(SEM)和液滴形貌分析仪(DSA)表征了TPU静电纺丝膜改性前后的组成、形貌以及润湿性的变化,证明MWCNT成功的固定到TPU静电纺丝膜上(2)SEM以及涂布平板法证明了TPU/MWCNT静电纺丝膜具有较好单层杀菌性能。第二:在TPU静电纺丝膜上构建双层杀菌表面。先将多壁碳纳米管的羧基活化,再与盐酸万古霉素(Van)的氨基反应,制成了Van-MWCNT粉末。然后将MWCNT、Van-MWCNT分别超声到TPU静电纺丝膜上,制备了TPU/MWCNT、TPU/Van-MWCNT静电纺丝膜。得到的主要结论如下:(1)表面全反射傅里叶红外光谱(ATR-FTIR)、XPS表征了TPU静电纺丝膜改性前后的表面化学性质,证明Van成功接枝到MWCNT。(2)ATR-FTIR、颜色变化、SEM和DSA表征了TPU静电纺丝膜改性前后的表面化学性质、颜色、形貌以及润湿性的变化,证明Van-MWCNT成功的固定到TPU静电纺丝膜上。(3)对金黄色葡萄球菌进行最低抑菌浓度测试,发现Van-MWCNT比MWCNT的最低抑菌浓度小,证明Van-MWCNT具有较好的杀菌性能。(4)SEM以及涂布平板法证明了TPU/Van-MWCNT静电纺丝膜具有较好的双层杀菌性能。第三:在TPU静电纺丝膜上构建抗杀结合的表面。通过紫外接枝的方法使具有抗污功能的聚乙二醇(PEG)接枝到TPU静电纺丝膜上,再利用超声波细胞粉碎机将MWCNT超声到TPU-g-PEG静电纺丝膜上,得到TPU-g-PEG/MWCNT静电纺丝膜。得到的主要结论如下:(1)X-射线光电子能谱仪(XRD)、DSA以及SEM表征了TPU静电纺丝膜改性前后的表面化学性质、润湿性和形貌的变化,证明了PEG成功的接枝到TPU静电纺丝膜上。(2)XRD、DSA、颜色变化以及SEM表征了TPU静电纺丝膜改性前后的表面化学性质、润湿性、颜色和形貌的变化,证明了MWCNT成功的固定到TPU-g-PEG静电纺丝膜上。(3)红细胞粘附和溶血测试,证明了PEG具有很好的抗粘附的功能并且减少了MWCNT对红细胞的毒性。(4)SEM以及涂布平板法证明了TPU-g-PEG/CNT静电纺丝膜不仅具有较好的抗污性能,还具有较好的杀菌性能。
[Abstract]:Dressings are materials used to cover wounds to provide an enabling environment for wound healing. As we all know, the wound is susceptible to various bacterial infections, leading to wound deterioration, pus, etc. Therefore, the dressing used to cover the wound should have a relatively good antibacterial properties, thus can be used to prevent wound infection, However, most of the dressings currently used do not meet the antibacterial requirements. In this paper, three kinds of antimicrobial surfaces were constructed on the TPU electrospun film based on the electrospun polyurethane film, and the structure, composition and antibacterial properties of the surface before and after modification were studied. The main contents are as follows: firstly, monolayer bactericidal surface was constructed on TPU electrostatic spinning film. The multiwalled carbon nanotubes (MWCNT) were immobilized on the TPU electrospinning film by ultrasonic dispersion method to prepare the TPU/MWCNT electrostatic spinning film. The main conclusions obtained are as follows: X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and droplet morphology analyzer (DSA) to characterize the composition, morphology and wettability of TPU films before and after modification. It was proved that MWCNT was successfully immobilized on TPU electrostatic spinning film and that TPU/MWCNT electrospun film had good monolayer bactericidal property. Second, double-layer sterilization surface was constructed on TPU electrostatic spinning film. Van-MWCNT powder was prepared by activating carboxyl group of multiwalled carbon nanotubes and reacting with vancomycin hydrochloride (Vann) amino group. Then the TPA / MWCNT / Van-MWCNT electrospun films were prepared by ultrasound on the TPU electrospun films. The main conclusions obtained are as follows: (1) the surface chemical properties of TPU electrospun films before and after modification were characterized by ATR-FTIR spectra. It was proved that Van was grafted to MWCNT.2AATR-FTIR.The changes of color were characterized by SEM and DSA. The changes of surface chemical properties, color, morphology and wettability of TPU films before and after modification were characterized. It was proved that Van-MWCNT was successfully immobilized on the electrostatic spinning membrane of TPU) to test the minimum inhibitory concentration of Staphylococcus aureus. It was found that the minimum inhibitory concentration of Van-MWCNT was lower than that of MWCNT. The results showed that Van-MWCNT had better bactericidal properties. The TPU/Van-MWCNT electrospun film had better double-layer bactericidal properties. Third, the surface of TPU electrostatic spinning film was constructed. Polyethylene glycol (PEG) with anti-fouling function was grafted onto TPU electrostatic spinning film by ultraviolet grafting method, and then MWCNT was ultrasonic onto TPU-g-PEG electrostatic spinning film by ultrasonic cell pulverizer, and TPU-g-PEG/MWCNT electrostatic spinning film was obtained. The main conclusions obtained are as follows: (1) SEM and SEM have characterized the changes of surface chemical properties, wettability and morphology of TPU electrospun films before and after modification. It was proved that PEG was successfully grafted onto the TPU electrospun film. The color change and the SEM characterization of the surface chemical properties, wettability, color and morphology of the TPU electrospun film before and after modification were demonstrated. It was proved that MWCNT was successfully immobilized on the electrostatic spinning membrane of TPU-g-PEG. It is proved that PEG has good anti-adhesion function and reduces the toxicity of MWCNT to erythrocyte. The coating plate method proves that TPU-g-PEG/CNT electrospinning film not only has good antifouling property, but also has better bactericidal property.
【学位授予单位】：聊城大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TQ340.64

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