紫山药主要化学成分测定、花青素提取及抗肿瘤活性的筛选

发布时间：2018-06-20 22:06

  本文选题：紫山药 + 化学成分　； 参考：《西南交通大学》2017年硕士论文

【摘要】：目的研究紫山药中的主要化学成分的含量测定、花青素的最佳提取方法、花青素稳定性以及抗肿瘤活性的筛选。方法①利用考马斯亮蓝法测定蛋白质含量、利用水提-醇沉法测定总糖的含量。②紫山药洗净,切片,采用酸性乙醇提取法、超声辅助提取法、加热辅助提取法进行粗提取。通过测出530 nm处吸光度,进行对比,取最大的吸光度值所采用的方法进行提取。再进行单因素实验及正交实验,确定提取花青素的最佳条件。③利用CCk-8法对HepG2肝癌细胞、MDA-MB-231乳腺癌细胞、RAW264.7巨噬细胞进行抗肿瘤活性筛选。结果①蛋白质含量为2.0%,多糖的含量为2.8%。②1kg紫山药中含有580 mg花青素。紫山药中的花青素的最佳提取工艺参数是:60℃,2%HCl-40%乙醇,提取40 min,料液比1:10。③花青素对HepG2肝癌细胞的IC50值是223μg/mL、对乳腺癌细胞的 IC50 值为 112.56μg/mL、对 RAW264.7 巨噬细胞的 IC50 值是 26.32μg/mL。结论①相比普通的山药品种,紫山药中多糖含量较高。②利用酸性乙醇溶液可以有效提取紫山药中的花青素。同时,对花青素提取影响最大实验因素是料液比。在酸性条件下,色素的稳定性较高。花青素在高温条件下容易分解。花青素对光照比较敏感。③花青素对HepG2肝癌细胞、MDA-MB-231乳腺癌细胞、RAW264.7巨噬细胞都有不同的抑制作用,其中对HepG2肝癌细胞、MDA-MB-231乳腺癌细胞、抑制作用低,对RAW264.7巨噬细胞抑制作用比较明显。
[Abstract]:Objective to study the content determination of the main chemical constituents, the optimum extraction method of anthocyanin, the stability of anthocyanin and the screening of antitumor activity in purple yam. Methods 1 the content of protein was determined by Coomassie brilliant blue method, and the content of total sugar was determined by water extraction and alcohol precipitation. The crude extraction was carried out by acid ethanol extraction, ultrasonic assisted extraction and heating assisted extraction. The absorbance at 530 nm was measured and compared, and the maximum absorbance was extracted by the method. The optimal conditions of anthocyanin extraction were determined by single factor experiment and orthogonal experiment. The anti-tumor activity of Raw264.7 macrophages was screened by CCk-8 method on MDA-MB-231 breast cancer cell line of HepG2. Results 1 the content of protein was 2.0 and the content of polysaccharide was 580mg anthocyanin in 2.8%.21kg purple yam. The optimum extraction parameters of anthocyanin in Violet yam were as follows: 1: 60 鈩，

本文编号：2045812