乌饭树树叶多糖的提

发布时间：2018-06-20 23:23

本文选题：乌饭树 + 多糖　；参考：《合肥工业大学》2017年硕士论文

【摘要】：乌饭树树叶含有多种活性成分。本文连续分级提取了乌饭树树叶多糖,测定了乌饭树树叶多糖的组成成分和基本特性,研究了乌饭树树叶多糖的流变学特性和乳化活性,分析了乌饭树树叶多糖的抗氧化活性,并对乌饭树树叶多糖四种组分的上述性质进行了比较。不同的提取方法会引起多糖的理化性质产生差异。乌饭树树叶经热缓冲液、螯合剂、稀碱溶液和浓碱溶液连续提取后得到四种多糖组分:HBSS、CHSS、DASS和CASS。傅里叶红外光谱分析表明乌饭树树叶多糖在3600 3200 cm-1和1200 800cm-1呈现糖类的特征吸收峰。高效液相色谱法测定提取的四种多糖组分的分子量分别为7.66×103,5.92×103,1.02×104,7.64×102 kDa。HBSS主要由阿拉伯糖、葡萄糖、半乳糖组成,CHSS主要由阿拉伯糖、半乳糖组成,DASS主要由鼠李糖、阿拉伯糖组成,CASS主要由阿拉伯糖、木糖、葡萄糖、半乳糖组成。在20-300°C温度范围内,CHSS表现出最高的峰值温度,HBSS具有最高的热焓。HBSS乳化活性最强,CASS乳化稳定时间最长。4种乌饭树树叶多糖的流变性质存在一定的差异。在质量浓度为0.6-2.5%的范围内,该多糖水溶液表现为典型的非牛顿流体剪切稀化特征。在pH为4.0或10.0,高温以及Ca~(2+)存在的条件下,该多糖溶液的表观粘度减小;而在低温或Na~+存在的条件下,其表观粘度会增加。4种乌饭树树叶多糖组分在抗氧化活性上有一定的差异。其中CASS具有最高的还原力;CHSS能有效地清除DPPH自由基;HBSS对羟基和ABTS自由基的清除能力最强;CHSS对亚铁离子的螯合能力相较于其他三种组分更强。上述对乌饭树树叶多糖理化特性和抗氧化性的研究,将来可能在功能食品中应用。
[Abstract]:The leaves of Eucalyptus chinensis contain a variety of active ingredients. In this paper, the polysaccharides from leaves of black rice were extracted continuously, the components and basic characteristics of polysaccharides from leaves were determined, the rheological properties and emulsifying activities of polysaccharides from leaves of black rice were studied, and the antioxidant activities of polysaccharides from leaves of black rice were analyzed. The above properties of four polysaccharides from leaves were compared. Different extraction methods may lead to different physicochemical properties of polysaccharides. Four kinds of polysaccharides were obtained by continuous extraction in hot buffer solution, chelating agent, dilute alkali solution and concentrated alkali solution. Fourier transform infrared spectroscopy (FTIR) analysis showed that polysaccharides from leaves of Prunus chinensis showed the characteristic absorption peaks of carbohydrates at 3600 ~ 3200 cm-1 and 1200 800cm-1. The molecular weight of the four polysaccharides extracted by HPLC was 7.66 脳 10 ~ 3N 5.92 脳 10 ~ 3N ~ (1.02) 脳 10 ~ (4) C ~ (7.64 脳 10 ~ 2) kDa.HBSS mainly consisted of arabinose, glucose and galactose, and its components were mainly composed of arabinose, galactose and rhamnose. Arabinose consists of arabinose, xylose, glucose and galactose. In the temperature range of 20-300 掳C, CHSS showed the highest peak temperature and HBSS had the highest heat enthalpy. HBSS emulsification activity was the strongest. There was some difference in rheological properties of polysaccharides from leaves of Cass with the longest time of emulsification and stabilization. In the range of 0.6-2.5%, the polysaccharide aqueous solution exhibits typical shear thinning characteristics of non-Newtonian fluids. The apparent viscosity of the polysaccharide solution decreased at pH 4.0 or 10.0, at high temperature and in the presence of CaTi2, while at low temperature or in the presence of Na ~, the apparent viscosity of the polysaccharide solution decreased. The apparent viscosity increased the antioxidant activity of 4. 4 species of leaves polysaccharides. Among them, Cass has the highest reductive power and CHSS can effectively scavenge DPPH free radical HBSS to hydroxyl radical and Abts radical. CHSS has stronger chelating ability to ferrous ion than the other three components. The above studies on the physicochemical properties and antioxidant properties of Polysaccharide from leaves may be used in functional foods in the future.
【学位授予单位】：合肥工业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TQ281

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