基于二氧化锆纳米颗粒载药体系的制备及应用研究
发布时间：2019-01-17 13:16
【摘要】：文章设计了第一个基于空心氧化锆纳米球的智能纳米容器(HMZSs-1),主要由中空介孔氧化锆纳米球(HMZSs)作为纳米支架和双稳态准轮烷作为超分子开关组成。使用HMZSs取代介孔二氧化硅纳米支架的显着优点是HMZSs可以经受热碱性反应环境,其提供了用于官能化的新颖策略,并且因此通过简单和可行的装配程序实现了双pH响应的控制释放功能。本文首先合成了双(2-氨基乙基)缩酮(PBAEK),并通过单保护将其改性至HMZSs的表面。通过透射电子显微镜(TEM)、氮气吸附脱附等温线(BET)、傅里叶红外光谱仪(FTIR)、固体核磁共振仪(SS 13C NMR)和热重分析仪(TG)等仪器对HMZSs的表面改性及结构进行了表征。使用了紫外分光光度计(UV)对HMZSs-1的释放性能进行了研究。发现在中性溶液下,葫芦[7]脲(CB[7])大环化合物与PBAEK络合形成[2]准轮烷作为超分子开关,阻断孔口并防止药物的泄漏。当溶液调节至碱性时,CB[7]大环化合物作为盖,与PBAEK链分离,并且由于离子-偶极相互作用的显着降低而打开开关,释放效率达到81%。当在酸性条件下,由于缩酮基团的断裂,PBAEK链被破坏,导致超分子开关的打开和随后的药物的释放,释放效率达到82%。具有酸碱双响应的控释特性的HMZSs-1预期应用于许多领域。本文中,研究了罗丹明B(RhB)负载的HMZSs-1作为药物递送系统的可行性。体外细胞研究表明,装载RhB的HMZSs-1可以容易地被SMMC-7721细胞摄取,在细胞内快速释放RhB,证明它们具有应用于药物化疗的潜力。
[Abstract]:The first intelligent nano-vessel (HMZSs-1) based on hollow zirconia nanospheres is designed in this paper, which consists of hollow mesoporous zirconia nanospheres (HMZSs) as nano-scaffolds and bistable quasirotane as supramolecular switches. The remarkable advantage of using HMZSs to replace mesoporous silica nanospheres is that HMZSs can withstand hot-alkaline reaction environments which provide novel strategies for functionalization. Therefore, the control and release function of double pH response is realized by a simple and feasible assembly program. In this paper, bis (2-aminoethyl) ketone (PBAEK), was synthesized and modified to the surface of HMZSs by single protection. Adsorption and desorption of nitrogen by transmission electron microscope (TEM),) isotherm (BET), Fourier transform infrared spectrometer (FTIR), The surface modification and structure of HMZSs were characterized by SS 13 C NMR and thermogravimetric analyzer (TG). The release performance of HMZSs-1 was studied by ultraviolet spectrophotometer (UV). It is found that in neutral solution, the macrocyclic compound of gourd [7] urea (CB [7]) complexed with PBAEK to form [2] quasiroalkane as a supramolecular switch to block the pore opening and prevent the leakage of the drug. When the solution is adjusted to alkalinity CB [7] macrocyclic compounds act as capping and separate from PBAEK chain and switch on due to the significant decrease of ion-dipole interaction. The release efficiency reaches 81%. Under acidic conditions, the PBAEK chain is destroyed due to the breaking of the Ketal group, which leads to the opening of supramolecular switch and the subsequent release of the drug, and the release efficiency is up to 82%. HMZSs-1 with acid-base dual-response controlled release properties is expected to be applied in many fields. In this paper, the feasibility of Rhodamine B (RhB) loaded HMZSs-1 as drug delivery system is studied. In vitro cellular studies showed that HMZSs-1 loaded with RhB could be easily ingested by SMMC-7721 cells. The rapid release of RhB, in cells proved their potential for drug chemotherapy.
【学位授予单位】：南京理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TQ460.6
本文编号：2410096
[Abstract]:The first intelligent nano-vessel (HMZSs-1) based on hollow zirconia nanospheres is designed in this paper, which consists of hollow mesoporous zirconia nanospheres (HMZSs) as nano-scaffolds and bistable quasirotane as supramolecular switches. The remarkable advantage of using HMZSs to replace mesoporous silica nanospheres is that HMZSs can withstand hot-alkaline reaction environments which provide novel strategies for functionalization. Therefore, the control and release function of double pH response is realized by a simple and feasible assembly program. In this paper, bis (2-aminoethyl) ketone (PBAEK), was synthesized and modified to the surface of HMZSs by single protection. Adsorption and desorption of nitrogen by transmission electron microscope (TEM),) isotherm (BET), Fourier transform infrared spectrometer (FTIR), The surface modification and structure of HMZSs were characterized by SS 13 C NMR and thermogravimetric analyzer (TG). The release performance of HMZSs-1 was studied by ultraviolet spectrophotometer (UV). It is found that in neutral solution, the macrocyclic compound of gourd [7] urea (CB [7]) complexed with PBAEK to form [2] quasiroalkane as a supramolecular switch to block the pore opening and prevent the leakage of the drug. When the solution is adjusted to alkalinity CB [7] macrocyclic compounds act as capping and separate from PBAEK chain and switch on due to the significant decrease of ion-dipole interaction. The release efficiency reaches 81%. Under acidic conditions, the PBAEK chain is destroyed due to the breaking of the Ketal group, which leads to the opening of supramolecular switch and the subsequent release of the drug, and the release efficiency is up to 82%. HMZSs-1 with acid-base dual-response controlled release properties is expected to be applied in many fields. In this paper, the feasibility of Rhodamine B (RhB) loaded HMZSs-1 as drug delivery system is studied. In vitro cellular studies showed that HMZSs-1 loaded with RhB could be easily ingested by SMMC-7721 cells. The rapid release of RhB, in cells proved their potential for drug chemotherapy.
【学位授予单位】：南京理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TQ460.6
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1 宫光彩;基于二氧化锆纳米颗粒载药体系的制备及应用研究[D];南京理工大学;2017年
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