人参鹿胎抗疲劳葡萄酒制备工艺及功能毒理学评价

发布时间：2019-02-22 12:18

【摘要】：本论文旨在研发一款针对体力疲劳有辅助保护作用的保健食品,研究以人参、鹿胎、玫瑰花、黄精为原料,以白砂糖为辅料制成的保健葡萄酒。对提取工艺进行考察,分别采用渗滤法、热回流法、冷浸法制备人参鹿胎抗疲劳葡萄酒,以总皂苷的含量以及葡萄酒的感官评价为指标综合评价,筛选出最佳制备方法。以料液质量比、提取时间、提取次数为因素设计正交试验,以上述指标为依据,优选出最佳工艺条件为采用冷浸法,正交实验最佳条件为料液质量比1:10、每次冷浸1天、冷浸2次。对制剂成型工艺的考察,分别考察离心转速、离心时间对样品产生沉淀的影响,确定最佳的离心条件确定最佳的离心条件为4000 r/min时间为6 min。多个样品中分别加入一定量的白砂糖或蜂蜜,根据感官评价得到最宜加入白砂糖,加入量为3g/100mL。对产品进行质量标准研究,考察紫外分光光度法测定人参鹿胎抗疲劳葡萄酒中总皂苷的含量的精密性、可重复性和加标回收率确定其线性范围,验证了该方法稳定、可行。对人参鹿胎抗疲劳葡萄酒进行功能学评价,各剂量组血清尿素氮含量与对照组比较均有降低,高剂量组与空白组的差别统计学意义,能够提升小鼠肝糖原储备能力,增加小鼠游泳时长,高剂量组与空白组差别有统计学意义;游泳后曲线下面积虽与对照组比较无统计学意义,但有下降的趋势,可见人参鹿胎抗疲劳葡萄酒具有缓解体力疲劳的功效。对人参鹿胎抗疲劳葡萄酒进行毒理学评价,急性毒性试验中MTD高于21 g/kg BW,属于无毒级;微核试验、Ames试验、精子畸形试验结果均为阴性;三十天喂养试验每项指标都没有发现不良影响。可见人参鹿胎抗疲劳葡萄酒安全可靠。
[Abstract]:The purpose of this paper is to develop a kind of health food which can protect physical fatigue, and study the health care wine made from ginseng, deer fetus, rose and yellow essence, and white sugar as auxiliary material. The extraction process was investigated and the best preparation method was obtained by synthetically evaluating the content of total saponins and the sensory evaluation of the wine by using percolation method heat reflux method and cold soaking method to prepare Panax ginseng deer fetal anti-fatigue wine with the content of total saponins as well as the sensory evaluation of the wine. The orthogonal experiment was designed with the mass ratio of material to liquid, extraction time and times of extraction as the factors. According to the above indexes, the optimum technological conditions were as follows: using cold leaching method. The optimum condition of orthogonal experiment was that the mass ratio of material to liquid was 1: 10, and the cold soaking was 1 day each time. Cold dip twice. The effects of centrifugal speed and time on the precipitation of the sample were investigated. The optimum centrifugation conditions were determined as 4000 r/min and 6 min.. A certain amount of white sugar or honey was added to several samples. According to the sensory evaluation, the most suitable addition of white sugar was 3 g / 100 mL. The quality standard of the product was studied. The precision of the determination of total saponins in ginseng deer fetal antifatigue wine by ultraviolet spectrophotometry was investigated. The repeatability and the recovery rate were determined. The method was proved to be stable and feasible. The functional evaluation of ginseng deer fetal antifatigue wine showed that the serum urea nitrogen content in each dose group was lower than that in the control group, and the difference between the high dose group and the blank group was statistically significant, which could improve the liver glycogen reserve ability of mice. Increase the swimming time of mice, the difference between the high dose group and the blank group was statistically significant; The area under the curve after swimming had no statistical significance compared with the control group, but it had a downward trend. It can be seen that ginseng deer fetal antifatigue wine has the effect of relieving physical fatigue. The toxicological evaluation of ginseng deer fetal antifatigue wine showed that MTD was higher than 21 g/kg BW, in acute toxicity test, but the results of micronucleus test, Ames test and sperm malformation test were all negative. No adverse effects were found in each index of the 30-day feeding trial. It can be seen that ginseng deer fetal anti-fatigue wine is safe and reliable.
【学位授予单位】：长春工业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TS262.6

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