超声波强化酶水解提取污泥蛋白质研究
发布时间：2019-05-13 16:00
【摘要】：近年来,随着污水处理厂大量建设,水体污染情况得到了有效的控制,但是随之而来的是大量剩余污泥的产生。如果不能对其进行合理有效的处理处置,就会给生态环境和人体健康带来巨大的危害。污泥中含有30~60%的蛋白质,对其进行合理的提取和利用有助于实现污泥的稳定化、资源化和减量化。本文以郑州市某城市污水处理厂剩余污泥为研究对象,首先分别采用单独超声波法和单独酶法对其进行水解,研究了这两种方法对污泥蛋白质提取的主要影响因素;在此基础上,探索了超声波预处理对酶解污泥提取蛋白质的强化效果。课题研究的主要结论如下:(1)采用超声波提取污泥蛋白,在优化条件下,以功率密度为2W/mL的超声波对含水率为94%的污泥辐射处理20min,其蛋白质的提取率为15.3%,污泥比阻由原来的1.7×1010S2/g减小为2.419×109S2/g,污泥的脱水性能提高了83%;(2)采用碱性蛋白酶对污泥进行水解提取蛋白,在污泥含水率为94%时,用NaOH调节其pH为8,加入2%(w/w)的碱性蛋白酶,在55℃下水解4h,可获的蛋白质的最高提取率为58%,此时污泥比阻为1.382×109S2/g,污泥脱水性能提高了90%;(3)采用优化条件下的超声波对污泥进行预处理后,用碱性蛋白酶进行水解,其优化酶解条件与单独酶解的优化条件是一致的;但是其蛋白提取率达到69%,较单独酶解提高了19%;污泥比阻为0.62×109S2/g,其脱水性能较单独酶解提高了55%,表明超声波预处理可以有效强化酶解提取污泥蛋白质过程;(4)超声波-酶法对蛋白质的提取率较碱热法低21%左右,但是污泥的脱水性能较碱热法高56%。其蛋白液成分中氨基酸的含量较高,适合做叶面肥、动物饲料等。且超声波-酶法反应条件温和,不产生二次污染,因此具有广阔的应用前景。
[Abstract]:In recent years, with the construction of sewage treatment plants, water pollution has been effectively controlled, but a large number of excess sludge has been produced. If it can not be dealt with reasonably and effectively, it will bring great harm to the ecological environment and human health. The sludge contains 30% 60% protein, and its reasonable extraction and utilization is helpful to realize the stabilization, resource utilization and reduction of sludge. In this paper, the excess sludge of a municipal wastewater treatment plant in Zhengzhou was taken as the research object. Firstly, the main influencing factors of these two methods on the protein extraction of sludge were studied by using single ultrasonic method and single enzyme method respectively. On this basis, the enhancement effect of ultrasonic pretreatment on protein extraction from hydrolyzed sludge was explored. The main conclusions of this study are as follows: (1) under the optimized conditions, the protein extraction rate of sludge with water content of 94% was 15.3% when the sewage sludge with water content of 94% was irradiated by ultrasonic wave with power density of 2W/mL under the optimized conditions, and the main conclusions of the study were as follows: (1) under the optimized conditions, the extraction rate of sludge protein was 15.3%. The specific resistance of sludge is reduced from 1.7 脳 1010S2/g to 2.419 脳 109S2 / g, and the dewatering performance of sludge is improved by 83%. (2) the protein was hydrolyzed and extracted by alkaline protease. When the moisture content of sludge was 94%, the pH was adjusted to 8 by NaOH, and 2% (w 鈮，
本文编号：2476018
[Abstract]:In recent years, with the construction of sewage treatment plants, water pollution has been effectively controlled, but a large number of excess sludge has been produced. If it can not be dealt with reasonably and effectively, it will bring great harm to the ecological environment and human health. The sludge contains 30% 60% protein, and its reasonable extraction and utilization is helpful to realize the stabilization, resource utilization and reduction of sludge. In this paper, the excess sludge of a municipal wastewater treatment plant in Zhengzhou was taken as the research object. Firstly, the main influencing factors of these two methods on the protein extraction of sludge were studied by using single ultrasonic method and single enzyme method respectively. On this basis, the enhancement effect of ultrasonic pretreatment on protein extraction from hydrolyzed sludge was explored. The main conclusions of this study are as follows: (1) under the optimized conditions, the protein extraction rate of sludge with water content of 94% was 15.3% when the sewage sludge with water content of 94% was irradiated by ultrasonic wave with power density of 2W/mL under the optimized conditions, and the main conclusions of the study were as follows: (1) under the optimized conditions, the extraction rate of sludge protein was 15.3%. The specific resistance of sludge is reduced from 1.7 脳 1010S2/g to 2.419 脳 109S2 / g, and the dewatering performance of sludge is improved by 83%. (2) the protein was hydrolyzed and extracted by alkaline protease. When the moisture content of sludge was 94%, the pH was adjusted to 8 by NaOH, and 2% (w 鈮，
本文编号：2476018
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