两种提取工艺对金福菇和羊肚菌多糖的影响
发布时间：2024-12-21 01:11
食用菌多糖具有诸多药理作用,是食用菌中重要的生物活性成分。众所周知的,提取方法会极大的影响食用菌多糖的化学结构,进而影响其生物活性,采用新型快速、有效的多糖提取技术对食用菌多糖具有重要意义。在本文中,我们研究了两种新提取技术对金福菇（Tricholoma lobayense）和羊肚菌（Morchella esculenta）多糖的提取效果,阐明了提取方法对其提取率、结构及生物活性的影响。主要研究内容如下:1.闪提对金福菇多糖结构及抗氧化性的影响以水为溶剂对金福菇多糖进行闪式提取,对获得的多糖TLH-G进行结构解析和抗氧化性研究。研究发现TLH-G是一种分子量为4.1×10～3 Da的杂多糖,总糖、蛋白质和糖醛酸的含量分别为99.10%、0.72%和13.85%。单糖组成为甘露糖、鼠李糖、葡萄糖醛酸、葡萄糖和半乳糖,摩尔比为1.00:0.33:0.56:14.13:2.92,其主链由→1)-β-D-Glcp-（6→和→1）-α-D-Galp-(3→构成。抗氧化性研究表明TLH-G的DPPH和ABTS自由基的清除的IC50值分别为0.43 mg/mL和>0.5 m...
【文章页数】：102 页
【学位级别】：博士
【文章目录】：
Acknowledgements
摘要
abstract
Chapter1.Introduction to polysaccharides from edible fungi and their extraction techniques
1.1.Medicinal mushrooms
1.2.Nutritious significance of mushrooms
1.3.Mushroom bioactives
1.3.1.Mushroom polysaccharides
1.3.1.1.Glucans
1.3.1.2.Chitin and chitosan
1.3.1.3.Mannans and mannoproteins
1.3.2.Other nutritious portions of mushrooms
1.4.Structural properties of mushroom polysaccharides
1.5.Conventional extraction methods for mushrooms
1.5.1.Solvent-assisted extraction(SAE)
1.5.1.1.Hydro-alcohol extractions
1.6.Emerging technologies for polysaccharide extraction
1.6.1.Ultrasound-assisted extraction(UAE)
1.6.2.Enzyme-assisted extraction(EAE)
1.6.3.Microwave-assisted extraction(MAE)
1.6.4.Accelerated-solvent Extraction(ASE)or pressurized liquid extraction(PLE)
1.6.5.Supercritical fluid extraction(SFE)
1.6.6.Pulsed-electric field extraction(PEF)
1.6.7.High-pressure homogenization extraction(HPH)
1.6.8.Flash-assisted extraction(FAE)
1.6.9.Hydrothermal extraction or subcritical water/pressurized hot water extraction
1.6.9.1.Mechanisms involved during hydrothermal extraction
1.6.9.2.Parameters affecting hydrothermal extraction
Aims and objectives
References
Chapter2.Structural elucidation and antioxidant activity of a novel heteroglycan from Tricholoma lobayense by flash extraction
2.1.Introduction
2.2.Experimental Section
2.2.1.Materials and apparatus
2.2.2.Extraction procedure
2.2.3.Deproteination and purification
2.2.4.Physical and chemical properties analysis
2.2.5.Analysis of monosaccharide composition
2.2.6.Methylation and GC-MS analysis
2.2.7.NMR spectroscopy
2.2.8.Antioxidant activity assays
2.2.8.1.DPPH radical scavenging assay
2.2.8.2.ABTS assay
2.3.Results and discussions
2.3.1.Physical and chemical properties of TLH-G
2.3.2.FT-IR analysis of TLH-G
2.3.3.TLH-G monosaccharide analysis and their effect on antioxidant activity
2.3.4.Structural characterization of TLH-G via GC-MS analysis
2.3.5 NMR analysis of TLH-G
2.3.6.Antioxidant activity evaluation by DPPH and ABTS assays
2.3.7.Glycosidic bond types in TLH-G polysaccharides
References
Chapter3.Extraction of polysaccharides from Tricholoma lobayense by hydrothermal methodology
3.1.Introduction
3.2.Experimental section
3.2.1.Materials and methods
3.2.2.Extraction of Tricholoma lobayense mushroom
3.2.3.Total carbohydrate and protein content
3.2.4.Extraction at ideal conditions
3.2.5.Purification of T.lobayense polysaccharides
3.2.6.Antioxidant activity assays
3.2.6.1.DPPH radical scavenging activity assay
3.2.6.2.ABTS radical scavenging activity assay
3.2.6.3.Hydroxyl radical scavenging activity assay
3.2.6.4.Reducing power
3.2.6.5.Superoxide radical scavenging activity assay
3.3.Results and discussions
3.3.1.Extraction parameters and optimization
3.3.2.Optimization of ratio of solid to liquid(g/mL)
3.3.3.Optimization of temperature
3.3.4.Optimization of extraction time
3.3.5.In vitro antioxidant assays
References
Chapter4.Effects of hydrothermal extraction on polysaccharides from Morchella esculenta
4.1 Introduction
4.2.Experimental Section
4.2.1.Materials and reagents
4.2.2.Extraction of mushroom
4.2.3.Purification of Morchella esculenta polysaccharides
4.2.4.Total carbohydrate and protein content analysis
4.2.5.In vitro Antioxidant activity assays
4.2.5.1.DPPH radical scavenging assay
4.2.5.2.ABTS radical scavenging assay
4.2.5.3.Reducing power
4.2.5.4.Hydroxyl radical scavenging assay
4.2.6.Ethanol gradient precipitation
4.2.7.HPLC analysis
4.2.8.CTAB treatment
4.3.Results and discussions
4.3.1.Total sugar,protein,and in vitro antioxidant activities of M.esculenta polysaccharides
4.3.2.Fractionation by ethanol gradient precipitation and their HPLC analysis
References
Chapter5.Conclusions
Publications
Author’s profile
本文编号：4018154
【文章页数】：102 页
【学位级别】：博士
【文章目录】：
Acknowledgements
摘要
abstract
Chapter1.Introduction to polysaccharides from edible fungi and their extraction techniques
1.1.Medicinal mushrooms
1.2.Nutritious significance of mushrooms
1.3.Mushroom bioactives
1.3.1.Mushroom polysaccharides
1.3.1.1.Glucans
1.3.1.2.Chitin and chitosan
1.3.1.3.Mannans and mannoproteins
1.3.2.Other nutritious portions of mushrooms
1.4.Structural properties of mushroom polysaccharides
1.5.Conventional extraction methods for mushrooms
1.5.1.Solvent-assisted extraction(SAE)
1.5.1.1.Hydro-alcohol extractions
1.6.Emerging technologies for polysaccharide extraction
1.6.1.Ultrasound-assisted extraction(UAE)
1.6.2.Enzyme-assisted extraction(EAE)
1.6.3.Microwave-assisted extraction(MAE)
1.6.4.Accelerated-solvent Extraction(ASE)or pressurized liquid extraction(PLE)
1.6.5.Supercritical fluid extraction(SFE)
1.6.6.Pulsed-electric field extraction(PEF)
1.6.7.High-pressure homogenization extraction(HPH)
1.6.8.Flash-assisted extraction(FAE)
1.6.9.Hydrothermal extraction or subcritical water/pressurized hot water extraction
1.6.9.1.Mechanisms involved during hydrothermal extraction
1.6.9.2.Parameters affecting hydrothermal extraction
Aims and objectives
References
Chapter2.Structural elucidation and antioxidant activity of a novel heteroglycan from Tricholoma lobayense by flash extraction
2.1.Introduction
2.2.Experimental Section
2.2.1.Materials and apparatus
2.2.2.Extraction procedure
2.2.3.Deproteination and purification
2.2.4.Physical and chemical properties analysis
2.2.5.Analysis of monosaccharide composition
2.2.6.Methylation and GC-MS analysis
2.2.7.NMR spectroscopy
2.2.8.Antioxidant activity assays
2.2.8.1.DPPH radical scavenging assay
2.2.8.2.ABTS assay
2.3.Results and discussions
2.3.1.Physical and chemical properties of TLH-G
2.3.2.FT-IR analysis of TLH-G
2.3.3.TLH-G monosaccharide analysis and their effect on antioxidant activity
2.3.4.Structural characterization of TLH-G via GC-MS analysis
2.3.5 NMR analysis of TLH-G
2.3.6.Antioxidant activity evaluation by DPPH and ABTS assays
2.3.7.Glycosidic bond types in TLH-G polysaccharides
References
Chapter3.Extraction of polysaccharides from Tricholoma lobayense by hydrothermal methodology
3.1.Introduction
3.2.Experimental section
3.2.1.Materials and methods
3.2.2.Extraction of Tricholoma lobayense mushroom
3.2.3.Total carbohydrate and protein content
3.2.4.Extraction at ideal conditions
3.2.5.Purification of T.lobayense polysaccharides
3.2.6.Antioxidant activity assays
3.2.6.1.DPPH radical scavenging activity assay
3.2.6.2.ABTS radical scavenging activity assay
3.2.6.3.Hydroxyl radical scavenging activity assay
3.2.6.4.Reducing power
3.2.6.5.Superoxide radical scavenging activity assay
3.3.Results and discussions
3.3.1.Extraction parameters and optimization
3.3.2.Optimization of ratio of solid to liquid(g/mL)
3.3.3.Optimization of temperature
3.3.4.Optimization of extraction time
3.3.5.In vitro antioxidant assays
References
Chapter4.Effects of hydrothermal extraction on polysaccharides from Morchella esculenta
4.1 Introduction
4.2.Experimental Section
4.2.1.Materials and reagents
4.2.2.Extraction of mushroom
4.2.3.Purification of Morchella esculenta polysaccharides
4.2.4.Total carbohydrate and protein content analysis
4.2.5.In vitro Antioxidant activity assays
4.2.5.1.DPPH radical scavenging assay
4.2.5.2.ABTS radical scavenging assay
4.2.5.3.Reducing power
4.2.5.4.Hydroxyl radical scavenging assay
4.2.6.Ethanol gradient precipitation
4.2.7.HPLC analysis
4.2.8.CTAB treatment
4.3.Results and discussions
4.3.1.Total sugar,protein,and in vitro antioxidant activities of M.esculenta polysaccharides
4.3.2.Fractionation by ethanol gradient precipitation and their HPLC analysis
References
Chapter5.Conclusions
Publications
Author’s profile
本文编号：4018154
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