同步辐射原位微流设备的研制及在蛋白质研究中的应用

发布时间：2018-01-12 14:03

本文关键词：同步辐射原位微流设备的研制及在蛋白质研究中的应用　出处：《中国科学院研究生院(上海应用物理研究所)》2016年博士论文　论文类型：学位论文

【摘要】：同步辐射小角X射线散射技术是解析物质结构信息的一种技术手段,因其特有优势,被广泛应用于凝聚态物理、生物化学、材料科学等学科领域。其中,蛋白质等生物大分子溶液就是同步辐射小角散射技术研究的重要体系之一。利用同步辐射小角X射线散射技术,可对溶液状态(更接近生理状态)下的生物大分子样品进行微观结构和形态的研究。为了进一步提升同步辐射小角X射线散射实验线站针对溶液样品的原位实验能力,拓展其在蛋白质等生物大分子结构研究中的应用,本论文主要的工作是结合具体的实验需求,研制可用于同步辐射小角X射线散射实验的原位微流设备,并采用研制的原位微流设备进行了蛋白质的微观结构变化的应用研究。主要的工作内容如下:1.设计并建立了一种可控微流体蠕动设备。采用LabVIEW软件编写了此蠕动设备的控制程序,通过实验对可控微流体蠕动设备的进样量、溶液粘度与蠕动速度的关系进行了标定,并将此设备安装于同步辐射小角X射线散射实验线站,使用溶菌酶蛋白质溶液样品,对该设备的防辐射损伤性能进行了实验验证。此外,还对该设备进行了硬件及软件的优化升级。2.设计并建立了一种温度跳变微流芯片原位研究设备。利用ANSYS CFX流体力学软件对多种微流通道结构的流体换热特性进行了有限元分析,根据数值分析结果,确定了微流芯片的加热方案,完成了微流芯片通道结构的物理设计,并提出了实际加工方案。通过荧光染料温度指示剂标定方法对微流芯片的跳变温度进行了标定,并根据实验结果对微流芯片原型设计进行了优化改进。最后,将微流设备安装于同步辐射小角X射线散射实验线站,进行了蛋白质的复折叠过程的时间分辨SAXS研究。本论文的工作内容以实际实验需求为起点,研制了两种用于同步辐射小角X射线散射实验的原位微流设备,推动了上海光源同步辐射实验技术在溶液样品中的应用,对提升同步辐射溶液样品原位小角散射实验能力具有重要的意义。
[Abstract]:Synchrotron small angle X ray scattering technique is a kind of technical means of analytical information of material structure, because of its unique advantages, is widely used in condensed matter physics, biochemistry, materials science and other fields. Among them, one of the important system of bio macromolecules such as protein solution is on synchrotron radiation scattering technique using synchrotron small angle. Radiation small angle X ray scattering technique, the solution state (closer to the physiological state of biological macromolecules) to study the sample microstructure and morphology. In order to further enhance the synchrotron small angle X ray scattering experimental station for in situ experiments of solution samples, expand its application in the research of protein structure of biological macromolecules in this paper, the main work is the combination of the specific requirements of the development of experiment, can be used for in situ synchrotron small angle X ray scattering experiment of micro flow equipment, and using research Application of in situ micro flow equipment for the microstructure change of the protein. The main contents are as follows: 1. the design and establishment of a controllable micro peristaltic fluid equipment. The control program is compiled by using LabVIEW software the peristaltic equipment, the amount of sample experiment of controllable micro peristaltic fluid equipment, the relationship between viscosity and creep the speed was calibrated, and the equipment installed in the synchrotron small angle X ray scattering experimental station, using lysozyme protein solution samples of radiation damage performance of the device was tested. In addition, the device is optimized for hardware and software upgrade.2. designed and built a temperature jump in situ study of microfluidic chip devices. Fluid microchannel structure of using ANSYS CFX CFD software the heat transfer characteristics of the finite element analysis, according to the results of numerical analysis,. The heating program set a microfluidic chip, completed the physical design of microfluidic chip channel structure, and put forward the actual processing scheme. Through the fluorescent dye temperature indicator calibration method to calibrate the temperature jump of microfluidic chips, and according to the experimental results of the prototype design of microfluidic chip is improved. Finally, the micro flow equipment installed at the synchrotron small angle X ray scattering experimental station, the complex folding of the time-resolved SAXS study. The content of the thesis to the actual experimental demand as the starting point, two for in situ synchrotron small angle X ray scattering experiment of micro flow equipment was developed and promoted the application of Shanghai the light source synchrotron radiation technology in solution, has an important significance for enhancing the ability of small angle X-ray scattering of synchrotron radiation solution samples in situ.

【学位授予单位】：中国科学院研究生院(上海应用物理研究所)
【学位级别】：博士
【学位授予年份】：2016
【分类号】：Q51;O434.1

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