藓叶卷瓣兰、芳香石豆兰和韭菜根的化学成分研究

发布时间：2018-04-12 10:17

本文选题：藓叶卷瓣兰 + 芳香石豆兰　；参考：《云南大学》2016年博士论文

【摘要】：本论文对藓叶卷瓣兰(Bulbophyllum retusiusculum)、芳香石豆兰(Bulbophyllum ambrosia)和韭菜(Allium tuberosum)根的化学成分进行了研究。实验中应用硅胶柱层析,反向(RP-18)柱层析,葡聚糖凝胶(Sephadex LH-20)柱层析,大孔树脂HPD101柱层析等多种分离材料和分离技术,同时应用波谱分析方法和化学方法,从以上3种植物中分离鉴定了93个化合物,其中新化合物17个。论文还对分离得到的部分化合物进行了活性筛选,发现了一些有明显活性的化合物。藓叶卷瓣兰(Bulbophyllum retusiusculum)是兰科(Orchidaceae)石豆兰属(Bulbophyllum)植物。目前对藓叶卷瓣兰(Bulbophyllum retusiusculum)的化学成分研究还未见报道。本论文对藓叶卷瓣兰的化学成分进行了深入研究,从中分离得到37个化合物,包括9个新化合物：retusiusines A-G (1-7), bulbotetusine (8), apigenin 6-C-α-arabino-furanosyl 8-C-a-arabinopyranoside (9)和28个已知化合物。主要化学成分为苯丙素类化合物、联苄类化合物、菲类和二氢菲类化合物。对9个新化合物进行了细胞毒活性筛选实验,结果表明化合物4,5和7具有较强的抗肝癌(SMMC-7721)活性,化合物4和5有较弱的抗白血病(HL-60)活性,化合物7具有一定的抗乳腺癌(MCF-7)活性。另外还对化合物1,2,4,9,14,21,22,24和26进行了抗菌活性筛选,结果显示对于白色念珠菌(C. albicans),化合物2有较强的抗菌活性,化合物22有中等强度的抗菌活性,化合物4,21,24和26也有微弱的抗菌活性；对枯草芽孢杆菌(B. subtilis),化合物21,22,24和26有微弱的抗菌活性。芳香石豆兰(Bulbophyllum ambrosia),是兰科(Orchidaceae)石豆兰属(Bulbophyllum)植物。具有清热,止咳的作用,主要用于治疗肺热咳嗽。目前对芳香石豆兰的化学成分研究还未见报道,本论文对芳香石豆兰的化学成分进行了深入分析。从中分离鉴定了30个化合物,包括1个新化合物amrosine (1)和29个已知化合物。该植物主要化学成分为苯丙素类化合物、联苄类化合物、菲类和二氢菲类化合物。韭(Allium tuberosum)为百合科葱属多年生草本植物,其茎、叶、花均可食用,是云南日常食用的蔬菜,也可用于治疗腹痛、腹泻、吐血、毒蛇咬伤、哮喘等。目前研究主要集中在韭菜籽,而对于韭菜根的研究较少,为了对韭菜进行深入的开发利用,本文对韭菜根的化学成分进行了研究。从中分离得到了26个化合物,其中有7个新化合物tuberosines A-G(1-7)和19个已知化合物。7个新化合物包括3个甾体皂苷化合物(1-3),1个苯丙酸甲酯苷类化合物(4),3个苯乙酮苷类化合物(5-7)。还对分离到的3个新的甾体皂苷化合物(1-3)进行了抗肿瘤活性研究,结果表明化合物3对肝癌(SMMC-7721)、肺癌(A-549)、结肠癌(SW480)、白血病(HL-60)和乳腺癌(MCF-7)均具有活性。对分离到的9个化合物(化合物1-5,8,18,23和24)进行了抗菌活性筛选,结果显示化合物23和24对枯草杆菌(B. subtilis,32 μg/mL)和大肠杆菌(E. coil,16 μg/mL)有一定的抗菌活性,化合物2对枯草杆菌(B. subtilis, 64 μg/mL)和大肠杆菌(E. coil,64 μg/mL)也有一定的抗菌活性,化合物3和18对枯草杆菌(B. subtilis,128μg/mL)有微弱的抗菌活性,其它化合物没有抗菌活性。本论文最后对2015年及以前发表的石豆兰属(Bulbophyllum)植物化学成分及药理活性研究进行了综述,共涉及105个化合物和31篇文献。
[Abstract]:The moss leaf cactus (Bulbophyllum retusiusculum), fragrant orchid (Bulbophyllum Ambrosia) and Shi Doulan (Allium tuberosum) the chemical composition of leek root was investigated by silica gel column chromatography experiment, reverse (RP-18) column chromatography, Sephadex column chromatography (Sephadex LH-20), HPD101 macroporous resin column chromatography and other separation materials and separation technology, at the same time the application of spectral analysis and chemical methods, from more than 3 kinds of plants in the isolation of 93 compounds, including 17 new compounds. The compounds were isolated from active screening, some obvious active compounds are found. Moss leaf cactus (Bulbophyllum retusiusculum) is blue Bulbophyllum genus of Orchidaceae (Orchidaceae) (Bulbophyllum) plants. The leaf moss orchid (Bulbophyllum retusiusculum) researched the chemical composition of the study has not been reported. In this paper, moss leaves The chemical composition of the orchid cactus were studied, 37 compounds have been isolated from, including 9 new compounds: retusiusines A-G (1-7), bulbotetusine (8), apigenin 6-C- -arabino-furanosyl 8-C-a-arabinopyranoside alpha (9) and 28 known compounds. The main chemical constituents, phenylpropanoids, bibenzyl compounds the Philippines, and two hydrogen Philippines compounds. 9 new compounds were cytotoxic activity screening experiments, the results showed that compounds 4,5 and 7 have strong anti hepatoma activity (SMMC-7721), 4 and 5 compounds have weak ANTI LEUKEMIA activity (HL-60), compound 7 has certain anti breast cancer (MCF-7) in addition to the activity of compounds 1,2,4,9,14,21,22,24 and 26 were screened for antibacterial activity, the results show that for Candida albicans (C., albicans) 2 compounds had strong antibacterial activity, 22 compounds have moderate antibacterial activity Of compounds 4,21,24 and 26 have weak antibacterial activity against Bacillus subtilis (B.; subtilis), compounds 21,22,24 and 26 have weak antibacterial activity. Shi Doulan (Bulbophyllum Ambrosia), fragrant orchid (Orchidaceae) is a species of Bulbophyllum (Bulbophyllum) plant. With heat, cough, mainly used for the treatment of lung heat cough. At present the studies on chemical constituents of fragrant Bulbophyllum have been reported, the chemical composition of aromatic Bulbophyllum odoratissimum were analyzed. From the isolation of 30 compounds, including 1 new compounds of amrosine (1) and 29 known compounds. The main chemical constituents of this plant phenylpropanoids compound bibenzyl compound, phenanthrene and two hydrogen Philippines compounds. (Allium tuberosum) for leek Allium perennial herbaceous plants, stems, leaves, flowers can be eaten, Yunnan daily consumption of vegetables, but also can be used for the treatment of Abdominal pain, diarrhea, vomiting, snake bites, such as asthma. The present research mainly focuses on the research of Chinese chive seed, leek root less, in order to carry on the development and utilization of Chinese chive in depth, the chemical composition of leek root were studied. We obtained 26 compounds isolated from, including 7 new compounds tuberosines A-G (1-7) and 19 known compounds.7 new compounds including 3 steroidal saponins compounds (1-3), 1 benzene methyl propionate glycosides (4, 3) acetophenone glycosides (5-7). 3 new steroidal saponins compounds on isolated (1-3) studied the antitumor activity, the results showed that the compound 3 on hepatocellular carcinoma (SMMC-7721), lung cancer (A-549), colon cancer (SW480), leukemia (HL-60) and breast cancer (MCF-7) were activated. 9 compounds isolated (compounds 1-5,8,18,23 and 24) were screened for antibacterial activity. The results showed that. 23 and 24 of Bacillus subtilis (B. subtilis, 32 g/mL) and Escherichia coli (E. coil, 16 g/mL) has certain antibacterial activity, 2 compounds of Bacillus subtilis (B. subtilis, 64 g/mL) and Escherichia coli (E. coil, 64 g/mL) also has certain antibacterial activity, compounds 3 and 18 of Bacillus subtilis (B. subtilis, 128 g/mL) has weak antibacterial activity, other compounds have no antibacterial activity. In the end of 2015 and previously published species of Bulbophyllum (Bulbophyllum) study on chemical constituents and pharmacological activities of plants were reviewed, involving a total of 105 compounds and 31 articles.

【学位授予单位】：云南大学
【学位级别】：博士
【学位授予年份】：2016
【分类号】：Q946

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