生物体系偏振测量系统的设计

发布时间：2018-04-18 05:16

  本文选题：偏振 + GLP　； 参考：《清华大学》2016年博士论文

【摘要】：偏振光能够探测生物组织的微观结构信息,而作为一种非标记、无损伤的检测技术,偏振成像技术已经在生物医学尤其是肿瘤癌症检测领域显示出潜在的应用前景。偏振方法的基础是偏振测量,快速稳定的偏振测量系统对偏振调制器件提出了较高的要求;同时,由于偏振测量具有复杂的误差源及误差传递性质,偏振测量系统必须经过合理的设计才能获得精确的测量结果。本论文对偏振测量系统的设计方法进行了系统的研究,提出了新型偏振调制器件和方法,并将偏振设计理论与偏振调制技术相结合进行了生物体系中偏振测量系统的设计。本论文以误差传递为线索,在降噪-优化-校准三个方面系统地研究了偏振测量系统的设计方法。本论文研究了全面评估偏振测量系统的方法,兼容现有优化指标;研究了检偏通道数目对优化的影响;归纳了偏振元件误差的校准方法,提出了非偏振元件偏振残差的校准方法,并在生物测量系统中对其进行了验证;提出了光束漂移的校准技巧及相应的60次穆勒矩阵测量方案;提出了最佳校准偏振态的选取方法。此外,由于待测体系通常具有特定的偏振特征,而且不同的偏振调制元件具有不同的精度,为了充分利用这两个特点,论文提出了分步式优化和分步式校准的思想。本论文提出了一种新型偏振调制器件和方法,发明了基于自聚焦透镜(GRIN lens)的GLP(GRIN lens polarimeter)偏振测量系统。GRIN lens不仅是波前调制器件,还是偏振调制器件。GLP偏振测量系统不仅能够实现单帧同时性偏振态测量,而且能够遍历所有可能的偏振调制方式,这意味着它不仅包含现有的各种偏振调制方式,还能开发出全新的偏振调制方式。本论文将偏振设计理论与偏振调制技术相结合,设计了生物体系中的新型偏振显微系统。基于分焦平面偏振相机的偏振显微系统既具备最佳的穆勒矩阵测量能力,又具备同时性斯托克斯向量成像能力。通过临床生物组织病理切片的偏振成像研究,验证了使用偏振显微系统进行快速癌症切片诊断的潜力。同时,本论文总结了将偏振测量方法应用于生物诊断的步骤:从局部偏振指标测量到全穆勒矩阵测量,再到有针对性的局部偏振指标测量。穆勒矩阵测量能够反映组织全部的偏振光学信息,而局部偏振指标测量往往更加快速和准确。
[Abstract]:Polarized light can detect the microstructure information of biological tissue. As a non-marker and non-injury-free detection technology, polarization imaging technology has shown a potential application in biomedicine, especially in the field of cancer detection.The polarization measurement method is based on polarization measurement, and the fast and stable polarization measurement system has put forward higher requirements for polarization modulation devices, at the same time, the polarization measurement has complex error sources and error transfer properties.Polarization measurement system must be properly designed to obtain accurate measurement results.In this paper, the design method of polarization measurement system is systematically studied, and a new polarization modulation device and method is proposed. The polarization measurement system in biological system is designed by combining polarization design theory with polarization modulation technology.Based on error transfer, the design method of polarization measurement system is studied systematically in three aspects: noise reduction, optimization and calibration.In this paper, the methods of comprehensive evaluation of polarization measurement system are studied, which are compatible with the existing optimization indexes; the influence of the number of polarization channels on the optimization is studied; and the calibration method of polarization component error is summarized.The calibration method of polarization residuals of non-polarization components is proposed and verified in the biometric system, and the calibration technique of beam drift and the corresponding 60 order Muller matrix measurement scheme are presented.A method for selecting the best calibrated polarization state is proposed.In addition, because the system under test usually has specific polarization characteristics and different polarization modulators have different precision, in order to make full use of these two characteristics, the idea of stepwise optimization and stepwise calibration is proposed in this paper.In this paper, a novel polarization modulation device and method is proposed. The polarization measurement system of GLP(GRIN lens polarimeter.GRIN lens is not only a wavefront modulation device based on self-focusing lens GRINLens.Or the polarization modulation device. GLP polarization measurement system can not only realize single frame simultaneous polarization state measurement, but also can traverse all possible polarization modulation modes, which means that it not only includes all kinds of polarization modulation methods,New polarization modulation can also be developed.In this paper, the polarization design theory and polarization modulation technology are combined to design a new polarization microscope system in biological system.The polarization microscopy system based on the focal plane polarization camera has both the optimal Muller matrix measurement capability and the simultaneous Stokes vector imaging capability.The potential of rapid diagnosis of cancer sections using polarization microscopy was verified by the study of polarization imaging of clinical pathological sections of biological tissues.At the same time, this paper summarizes the steps of applying polarization measurement to biological diagnosis: from local polarization index measurement to full Muller matrix measurement, and then to targeted local polarization index measurement.Muller matrix measurement can reflect all the polarized light information of tissue, and the measurement of local polarization index is more rapid and accurate.
【学位授予单位】：清华大学
【学位级别】：博士
【学位授予年份】：2016
【分类号】：O436.3
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本文编号：1766953