细胞介电泳空间操作及微组装的自动化技术研究

发布时间：2024-05-21 20:07

　　组织工程学是一门多学科交叉的研究领域,其目的是在体外构建人造组织,然后将其移植到体内进行组织的修复和再生。组织的构造通常包括两个主要的步骤,首先是从病人的骨髓中取得干细胞,随后将这些细胞在生物相容性的支架上进行培养以此来促使组织的发育。然而大多数现有的支架结构都过于简单,难以控制细胞的分布。本论文中,我们设计了新型的生物支架结合介电泳技术来实现细胞在支架上的自动种植。本文由以下三个方面展开:首先,提出了一种由多层生物相容性材料构成的新型生物支架,该支架结合介电泳技术实现了对生物细胞的三维操作和排列。我们检验了不同的工艺参数对三维细胞构型的质量和细胞成活率的影响。实验结果表明我们所提出的三维支架能够利用介电泳力机理实现细胞的自动排列,从而构建组织工程学上所需的人造组织。不同的外加电压所产生的电场力对被操作生物细胞生存率的影响也做了评估,选择了最合适的外加电场。其次,设计了一种蜂窝状的模拟原始骨组织结构的多孔生物支架。该支架能够自动将细胞排列成多层的蜂窝状构型。我们使用三种不同的哺乳动物的细胞进行实验,以检测影响蜂窝状细胞构型形成过程中的不同因素。我们还对材料的生物相容性进行了测试,以保证...

【文章页数】：112 页

【学位级别】：博士

【文章目录】：
摘要
ABSTRACT
Chapter 1 Introduction
    1.1 Background
    1.2 Statement of Problems
    1.3 Research Objectives
    1.4 Methodology and Significance
    1.5 Conclusion
Chapter 2 Literature Review
    2.1 Introduction
    2.2 Cell Manipulation Technologies
        2.2.1 Optical Tweezers
        2.2.2 Magnetic
        2.2.3 DEP
        2.2.4 Mechanical
    2.3 Microfabrication Techniques
        2.3.1 2D Planar Electrode Fabrication
        2.3.2 3D Electrode Fabrication
    2.4 Different Electrodes for DEP Manipulation
        2.4.1 2D Planar Microelectrodes
        2.4.2 3D Microelectrodes
    2.5 Conclusion
Chapter 3 Development of Multilayer Scaffold for 3D Cell Manipulation andPatterning
    3.1 Introduction
    3.2 Materials and Methods
        3.2.1 Multilayer Scaffold Structure Design
        3.2.2 Scaffold Fabrication and Assembly
        3.2.3 Hardware Setup
    3.3 Results and Discussions
        3.3.1 Effect of Supply Voltage Input on Multilayer Structure
        3.3.2 Characterization of 3D Cellular Pattern
        3.3.3 Biocompatibility of Scaffold for Cell Culture
        3.3.4 Assessment of Cell Viability after Cell Patterning by DEP
        3.3.5 Assessment of Cellular Pattern after Culture
    3.4 Conclusions
Chapter 4 Characterization of Honeycomb-patterned Scaffold with DEP-basedPatterning for Bone TE
    4.1 Introduction
    4.2 Materials and Methods
        4.2.1 Scaffold Design
        4.2.2 Fabrication
        4.2.3 Cell Culture
        4.2.4 Cell Preparation for DEP Manipulation
        4.2.5 Experimental Setup for DEP-based Cell Patterning
    4.3 Results and Discussion
        4.3.1 Effects of Input Voltage Frequency
        4.3.2 Effects of Material on Cell Culture
        4.3.3 Cell Viability during DEP Patterning
        4.3.4 Patterning Efficiency
    4.4 Conclusions
Chapter 5 Development of Engineered Bone Scaffolds with DEP-basedPatterning utilizing 3D Printing
    5.1 Introduction
    5.2 Materials and Methods
        5.2.1 Scaffold Design
        5.2.2 Scaffold Fabrication
        5.2.3 Cell Preparation
        5.2.4 DEP Cell Patterning
        5.2.5 Bone Nodule Formation
    5.3 Results and Discussion
        5.3.1 Scaffold Characterization
        5.3.2 DEP Patterning
        5.3.3 Bone Nodule formation
    5.4 Conclusions
Chapter 6 Conclusion and Future Work
    6.1 Conclusion
    6.2 Future Work
Chapter 7 结束语
    7.1 主要工作与贡献
    7.2 研究前景和展望
Reference
致谢
在读期间发表的学术论文与取得的其他研究成果
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