Wip1在糖尿病视网膜病变前膜中的表达

发布时间：2017-12-27 05:17

  本文关键词：Wip1在糖尿病视网膜病变前膜中的表达　出处：《广西医科大学》2017年硕士论文　论文类型：学位论文

  更多相关文章： Wip1 视网膜前膜 增生性糖尿病视网膜病变 NF-κB 胶质细胞

【摘要】：目的:通过检测野生型P53诱导的磷酸酶1(Wild-type p53-induced phosphatase 1,Wip1)在增生性糖尿病视网膜病变前膜中的表达,同时分析Wip1和核转录因子κB(nuclear factor kappa-B,NF-κB)、Wip1和胶质细胞在PDR前膜中的定位关系,初步探讨其参与增生性糖尿病视网膜病变前膜形成的机制。方法:从玻璃体切割术中获取患者视网膜前膜组织,取增生性糖尿病视网膜病变患者的前膜作为实验组(PDR组),取不合并其他眼病患者的特发性视网膜前膜(idiopathic epiretinal membrane,i ERM)作为对照组(iERM组)。利用逆转录聚合酶链反应(RT-PCR)检测前膜组织中Wip1的mRNA表达。用免疫组化及免疫荧光法检测前膜中Wip1的蛋白表达。运用免疫荧光双染法观察Wip1和GFAP(视网膜胶质细胞的标记物),以及Wip1和NF-κB的共定位关系。结果:共收集26例视网膜前膜组织,其中PDR组17例,iERM组9例。RT-PCR结果显示Wip1的mRNA在PDR组中呈高表达,而在iERM组中仅有微弱表达。免疫组化和免疫荧光结果均显示Wip1在PDR组的前膜中有较强的阳性信号。通过免疫荧光双染发现Wip1和GFAP、NF-κB在PDR组前膜中的表达有相关性。结论:本研究结果说明Wip1可能通过其与NF-κB的相互作用参与了增生性糖尿病视网膜病变中纤维血管膜的形成。
[Abstract]:Objective: through the detection of wild type P53 induced phosphatase 1 (Wild-type p53-induced 1 phosphatase, Wip1) expression in proliferative diabetic retinopathy before in the film, and the analysis of the Wip1 and nuclear factor kappa B (nuclear factor kappa-B, NF- K B), relationship between the localization of Wip1 and glial cells in the PDR membrane in the preliminary to explore the mechanism of the participation of proliferative diabetic retinopathy before film formation. Methods: from the patients with epiretinal membrane cutting obtain vitreous body tissue, membrane from patients with proliferative diabetic retinopathy as experimental group (PDR group), were not associated with other eye disease in patients with idiopathic epiretinal membrane (idiopathic epiretinal membrane, I ERM) as control group (group iERM). The mRNA expression of Wip1 in the anterior membrane was detected by reverse transcriptase polymerase chain reaction (RT-PCR). Immunofluorescence and immunofluorescence were used to detect the protein expression of Wip1 in the anterior membrane. Wip1 and GFAP (the markers of retinal glia) and the co localization of Wip1 and NF- kappa B were observed by double staining with immunofluorescence. Results: a total of 26 cases of retinal anterior membrane were collected, including 17 cases in group PDR and 9 in group iERM. The RT-PCR results showed that the mRNA of Wip1 was highly expressed in the PDR group, but only in the iERM group. The results of immunohistochemistry and immunofluorescence showed that Wip1 had a strong positive signal in the anterior membrane of group PDR. The expression of Wip1, GFAP and NF- kappa B in the anterior membrane of group PDR was correlated by double staining of immunofluorescence. Conclusion: the results of this study suggest that Wip1 may be involved in the formation of fibrous vascular membrane in proliferative diabetic retinopathy through its interaction with NF- kappa B.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R587.2;R774.1

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