BTBD7及E-cadherin在唾液腺腺样囊性癌中的表达与相关性的研究

发布时间：2018-01-14 08:14

  本文关键词：BTBD7及E-cadherin在唾液腺腺样囊性癌中的表达与相关性的研究　出处：《山东大学》2017年硕士论文　论文类型：学位论文

  更多相关文章： BTBD7 E-cadherin 唾液腺 腺样囊性癌 免疫组织化学

【摘要】：目的:检测BTBD7和E-cadherin在唾液腺腺样囊性癌(SACC)中的表达情况,并研究其与SACC不同临床病理特性的相关关系,同时研究二者之间的相关关系。材料和方法:应用免疫组织化学的方式,将本研究搜集的50例SACC组织标本及10例正常唾液腺组织标本进行染色处理,分别检测其中BTBD7及E-cadherin的表达情况。抗BTBD7抗体结合位置为细胞浆,抗E-cadherin抗体结合位置除细胞浆外,同时有细胞膜;细胞质内出现大量的棕黄色颗粒状物质着色判定为BTBD7阳性表达的标准,细胞质及细胞膜表面同时出现明显的棕黄色颗粒状物质显色为E-cadherin阳性表达的标准。用统计学软件SPSS 23.0分析实验结果,将50例组织标本按照各临床病理特征(病理类型、神经侵犯、血行转移、临床分期)进行分组,采取x2检验和Fisher确切概率法检验差异,采取Spearman相关分析检验BTBD7及E-cadherin的关联性;结果以P0.05标记为存在统计学差异。结果:(1)与正常唾液腺组织比较,在唾液腺腺样囊性癌组织中BTBD7表达显著增加,在50例肿瘤组织中有21例表达呈阳性,其阳性表达率为420%,两者之间具有统计学差异(P=0.011)。BTBD7在低分化SACC中表达增加;在Ⅰ期、Ⅱ期与Ⅲ期、Ⅳ期之间具有统计学差异(P=0.002)。BTBD7在有神经浸润的SACC组织中阳性表达率高达63.640%,明显高于无神经浸润的肿瘤组织(21.430%),其差异具有显著性(P=0.002)。在16例有血行性转移的唾液腺腺样囊性癌组织中BTBD7的阳性表达率为62.5%,显著高于无转移的肿瘤组织,有统计学意义(P=0.022)。(2)与正常唾液腺组织比较,在SACC组织中E-cadherin表达下降(62%),其差异具有统计学意义(P=0.018)。E-cadherin在低分化SACC中表达减少,在Ⅰ期、Ⅱ期与Ⅲ期,Ⅳ期之间的差异具有统计学意义(P=0.002)。E-cadherin在有神经浸润的SACC组织中阳性表达率为45.45%,明显低于无神经浸润的癌组织(78.57%),其差异有统计学意义(P=0.015)。在有血行性转移的SACC组织中E-cadherin的阳性表达率为37.50%,低于无转移的癌组织(75.86%),差异具有显著性(P=0.011)。(3)在50例SACC样本中,有8例表现为BTBD7与E-cadherin共同阳性,6例为共同阴性,二者单独阳性的例数分别有23例和6例,经过Spearman相关分析检验,二者的表达在SACC中呈现负相关(r=-0.419,P=0.002)。结论:BTBD7在SACC中呈现高表达,E-cadherin呈现低表达,且二者与SACC的病理分期、有无神经浸润及有无远处转移存在相关性;同时BTBD7和E-cadherin在SACC组织中的表达呈现负相关。意义:本课题研究了 BTBD7与E-cadherin在唾液腺腺样囊性癌中的表达情况,结果发现二者与SACC的病理分期、神经侵犯、远处转移均相关,同时二者在SACC组织中表达呈现负相关,同时检测两者也许可为研究SACC细胞的生物学行为提供参考指标,为临床治疗SACC提供新思路。
[Abstract]:Objective: to detect the expression of BTBD7 and E-cadherin in salivary adenoid cystic carcinoma (SCC) and to study the relationship between them and the different clinicopathological characteristics of SACC. Materials and methods: 50 SACC tissues and 10 normal salivary gland tissues collected in this study were stained by immunohistochemistry. The expression of BTBD7 and E-cadherin were detected respectively. The binding position of anti-#en1# antibody was cytoplasm, and the binding position of anti-E-cadherin antibody was except cytoplasm. There is cell membrane at the same time; A large number of brown-yellow granules in the cytoplasm were identified as positive expression of BTBD7. The standard of E-cadherin positive expression was observed on the surface of cytoplasm and cell membrane. The results were analyzed by statistical software SPSS 23.0. According to the clinicopathological features (pathological type, nerve invasion, blood metastasis, clinical stage), 50 tissue specimens were divided into two groups. The difference was detected by x2 test and Fisher exact probability method. The correlation between BTBD7 and E-cadherin was examined by Spearman correlation analysis. Results compared with normal salivary gland tissue, the expression of BTBD7 in salivary adenoid cystic carcinoma was significantly increased. Of the 50 cases, 21 were positive, and the positive rate was 420%. There was a significant difference between the two groups in the expression of PTEP 0.011. BTBD7 in poorly differentiated SACC. There was statistical difference between stage 鈪，

本文编号：1422750