基于数据挖掘分析急性脑出血用药规律及涤痰通瘀方对Kupffer细胞的干预研究

发布时间：2018-01-16 00:12

本文关键词：基于数据挖掘分析急性脑出血用药规律及涤痰通瘀方对Kupffer细胞的干预研究　出处：《北京中医药大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:1.基于中医传承辅助系统(V2.5)软件,通过文献挖掘,研究中医药治疗急性脑出血的组方规律,为中医药治疗急性脑出血提供参考和依据。2.通过对急性脑出血大鼠Kupffer细胞的实验研究,探讨涤痰通瘀方对其保护作用及对白细胞介素-1受体相关激酶-M(Interleukin-1 Receptor Associated Kinase-M,IRAK-M)相关表达的影响,为涤痰通瘀方的临床应用提供一定的实验基础。方法:1.文献挖掘:筛选1991年-2016年相关期刊论文(CNKI)中有关中医药治疗急性脑出血文献中的方剂,应用中医传承辅助系统软件,运用无监督数据挖掘方法,挖掘高频药物、药对、核心组合、新组处方等,探讨治疗急性脑出血的组方规律。2.实验研究:采用尾状核注射Ⅶ型胶原酶法建立大鼠脑出血模型,将健康SD大鼠随机分为正常对照组、脑出血模型组和涤痰通瘀组(酒大黄、胆南星、石菖蒲、蒲黄、三七、水蛭),采集血清,-20℃冻存备用。密度梯度离心法提取正常大鼠Kupffer细胞(Kupffercells,KCs),给药后培养 6、12、24h。PCR 检测 IRAK-MmRNA 基因表达,Western blot检测NF-κB蛋白变化,Elisa检测TNF-α表达水平,流式细胞技术检测KCs凋亡。结果:1.文献挖掘:筛选出符合标准的文献共计312篇,收录方剂318首,涉及中药206味,按照药物频次由高到低排列前十位药物依次为大黄、三七、石菖蒲、水蛭、丹参、钩藤、牛膝、胆南星、天麻、川芎,其中大黄的用药频率最高(199次);得到核心组合7组,药物组合中以大黄为核心;核心组合中以大黄、三七组合的用药频次最高(101次);此外,得到新处方7个。2.实验研究:涤痰通瘀组IRAK-M mRNA表达水平与正常组相比略降低,但是较脑出血模型组明显升高(P0.05);与脑出血模型组相比,涤痰通瘀组可以显著降低KCs中NF-κB、TNF-α水平(P0.05,P0.01);与正常对照组相比,脑出血模型组可见较多凋亡细胞,涤痰通瘀组KCs凋亡率较脑出血模型组显著减少(P0.05)。结论:1.通过数据挖掘方法分析治疗急性脑出血组方规律,大黄的应用最广泛;核心组合以大黄、三七为主;药物种类以清热解毒、通腑化痰、活血化瘀药物居多。中医传承辅助平台系统软件在中医药对急性脑出血的临床文献研究应用,具有一定的指导意义。2.涤痰通瘀方可以提高KCs内IRAK-M mRNA的表达,阻滞NF-κB的活化,减少TNF-α的释放,有效抑制KCs凋亡,对急性脑出血应激性肝损害具有一定的保护作用。
[Abstract]:Objective: 1. TCM inheritance system (V2.5) based on software, through literature mining, prescription law research of traditional Chinese medicine in the treatment of acute cerebral hemorrhage, and provide reference for.2. through the experimental study on rat Kupffer cells in acute cerebral hemorrhage acute cerebral hemorrhage for the treatment of Chinese medicine, phlegm and blood stasis on the protective effect and interleukin -1 receptor associated kinase -M (Interleukin-1 Receptor Associated Kinase-M, IRAK-M) related to the expression, provide some experimental basis for clinical application of phlegm and Blood Stasis Decoction. Methods: 1. literature mining: screening 1991 -2016 China Journal Full-text Database (CNKI) bleeding prescription in literature about traditional Chinese medicine the treatment of acute cerebral, application of TCM inheritance system software, using unsupervised data mining method, mining high frequency drugs, drug combination, core, new prescription, treatment of acute cerebral hemorrhage group Experimental researches on the rules of.2.: to establish a rat model of intracerebral hemorrhage by injection of collagenase into caudate nucleus, healthy SD rats were randomly divided into normal control group, model group and blood stasis group phlegm in cerebral hemorrhage (Jiu Dahuang, bile Arisaema, Shichangpu, Puhuang, 37, leech), serum -20, C cryopreserved. From normal rat Kupffer cells by density gradient centrifugation (Kupffercells, KCs), to detect the expression of IRAK-MmRNA gene of 6,12,24h.PCR cultured after administration of Western blot detection of NF- kappa B protein changes, Elisa detection of TNF- expression level, flow cytometry to detect the apoptosis of KCs. Results: 1. literature mining: Selected to meet the standard the documents included a total of 312 articles, 318 prescriptions, involving 206 herbal medicines, drugs according to the frequency from high to low ranked the top ten drugs were 37, Shichangpu, rhubarb, leech, salvia, Achyranthes, uncaria, bile Arisaema, Rhizoma Gastrodiae, Rhizoma Chuanxiong, rhubarb with The highest frequency of drugs (199); a central group of 7 combinations of drugs, in combination with rhubarb as the core; core combination with rhubarb, 37 combined medication were the highest (101); in addition, get a new prescription 7.2. experimental research: phlegm and blood stasis group IRAK-M mRNA expression level was slightly decreased compared with the normal group, but compared to the cerebral hemorrhage model group increased significantly (P0.05); compared with the cerebral hemorrhage model group, phlegm and blood stasis group can significantly reduce KCs NF- kappa B, TNF- levels (P0.05, P0.01); compared with the normal control group, cerebral hemorrhage model group showed more apoptotic cells, phlegm and blood stasis group the apoptosis rate of KCs compared to the cerebral hemorrhage model group decreased significantly (P0.05). Conclusion: 1. through the analysis of data mining method in treatment of acute cerebral hemorrhage of prescription, the most widely used Rhubarb; core combined with rhubarb, 37; drug types to detoxify, Tongfu phlegm, promoting blood circulation drug are traditional Chinese medicine. The auxiliary platform system software in traditional Chinese medicine on acute cerebral hemorrhage clinical research literature, which has a certain guiding significance for removing phlegm and blood stasis.2. recipe can improve the expression of KCs in IRAK-M mRNA, block NF- kappa B activation, reduce TNF- release, inhibit the apoptosis of KCs, has a protective effect on stress liver injury in patients with acute cerebral hemorrhage.

【学位授予单位】：北京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R277.7

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