高压电烧伤大鼠血清SOD、MDA的变化及乌司他丁的干预作用

发布时间：2018-01-16 15:14

  本文关键词：高压电烧伤大鼠血清SOD、MDA的变化及乌司他丁的干预作用　出处：《河北医科大学》2017年硕士论文　论文类型：学位论文

  更多相关文章： 烧伤 高压电 超氧化物岐化酶 丙二醛 乌司他丁 氧化应激 大鼠

【摘要】：目的:高压电通过电热效应、电化学效应及电场力等作用,对机体神经、血管、肌肉等组织及器官造成损伤。高压电烧伤致伤的病理及生理机制尚未完全明了。研究认为,氧化应激参与高压电对机体的损伤过程。研究表明,烧伤后机体产生大量活性氧(reactive oxygen species,ROS),活性氧通过脂质过氧化等反应损伤组织和器官,并与炎症因子和血管活性物质协同作用,造成氧化应激损伤。高压电烧伤导致全身氧化应激反应的因素很多,其中血浆超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)在调节氧化应激方面有重要作用。SOD是广泛存在于生物体的金属氧化酶,能催化超氧阴离子自由基的歧化反应,通过清除细胞产生的超氧离子而发挥作用。MDA是脂质过氧化反应的产物。目前,关于高压电烧伤后大鼠血清SOD、MDA定量及定性变化的相关研究较少,本研究检测高压电烧伤大鼠血清SOD、MDA含量的变化,并采用乌司他丁(Ulinastatin,UTI)进行干预,旨在探索大鼠高压电烧伤后血清SOD、MDA的变化规律,证明氧化应激在高压电烧伤渐进性损伤发生和发展过程中的作用,探索UTI对高压电烧伤的治疗效果。方法:1实验动物分组:本实验用成年SD大鼠240只,大鼠由河北医科大学动物实验中心提供(合格证编号832457),体重291-362g,按随机区组设计法将其随机分为四组,每组60只,即假高压电烧伤组(假伤组)、高压电烧伤组(电伤组)、高压电烧伤UTI治疗组(UTI组)、高压电烧伤盐水治疗组(盐水组)。按随机数字表法,每组按观察时相分为电伤前15min、电伤后5min、电伤后1h、2h、4h、8h六个时相组,每一时相组10只大鼠。2实验前准备:(1)将实验大鼠称重并给予其编号登记;(2)准备实验用采血管、采血针等物品,记录实验数据;(3)按药品说明配置实验药品使其达到实验所需浓度。3高压电烧伤动物模型制备:用10%的水合氯醛(0.3ml/100g)溶液注入大鼠腹腔以麻醉实验大鼠,待麻醉成功后,将麻醉后大鼠仰卧固定于专用的电击实验台上。大鼠左上肢及右下肢脱毛处理,大鼠的左上肢(电流入口)及右下肢(电流出口)分别连接1cm×1cm电极片。将实验变压器和调压器电线相连接;再接通电源,调节调压器指针,将升压器输出电压调至2kv,接通升压器输出电源开关,使高压电流通过大鼠致电击,持续电击3s,高压电烧伤模型制作完成。将电伤后大鼠分笼放好,待采血。假伤组不予通电,其余处理步骤与电伤组及uti组的相同。盐水组电后5min内注射生理盐水5ml/kg至大鼠腹腔内,uti组电后5min内注射uti(2ml/kg)至大鼠腹腔内。每组样品按时相进行采血、分离血清。4标本采集与保存:将高压电烧伤大鼠再次麻醉,待麻醉后开胸暴露心脏,于心脏内直接抽血取样,血量约6ml,将抽出的血液注入促凝管中,静置30min,待血清析出后,放入离心机,调整转速3000转/min,离心10min,取上清液置于eppendorf管中于-70℃条件下保存。5指标检测:采用酶联免疫吸附双抗体夹心法(enzymelinkedimmunosorbentassay,elisa),分别检测每组大鼠的六个时相组血清sod、mda含量。6实验数据处理:采用spss21.0统计软件,行两因素析因设计的方差分析,多重比较采用lsd法t检验。以p0.05为显著性检验水准。结果:1大鼠血清sod含量变化电伤组大鼠血清sod含量与假伤组差异有显著性,总体低于假伤组(主效应f=165.245,p0.05);电伤组血清sod含量各时相变化差异有显著性(主效应f=16.905,p0.05),伤后5min~8h各时相均低于本组伤前值(p0.05),且呈逐渐降低趋势,sod含量在8h时相时达到最低,但电伤组sod含量仍较假伤组同时相低。血清sod含量uti组与盐水组差异有显著性,总体高于盐水组(主效应f=31.043,p0.05);uti组sod含量各时相变化差异有显著性(主效应f=62.830,p0.05),uti组sod含量伤后5min~8h各时相均高于本组伤前值(p0.05),且呈逐渐降低趋势。2大鼠血清mda含量变化电伤组血清mda含量与假伤组差异有显著性,总体高于假伤组(主效应F=1586.203,P0.05);电伤组MDA含量各时相变化差异有显著性(主效应F=126.552,P0.05),伤后5min~8h呈逐渐增高趋势,且各时相均高于本组电伤前值(P0.05)。UTI组血清MDA含量与盐水组差异有显著性,总体低于盐水组(主效应F=45.326,P0.05);UTI组MDA含量各时相变化差异有显著性(主效应F=246.201,P0.05),UTI组伤后5min~8h各时相均高于本组伤前值(P0.05),且呈逐渐增高趋势。结论:高压电烧伤使大鼠血清SOD含量降低、MDA含量升高,诱导高压电烧伤后氧化应激反应。高压电烧伤后采用UTI治疗,可使血清SOD的含量增加,MDA含量降低,表明UTI可以抑制高压电烧伤后的氧化应激反应,减轻氧化应激对高压电烧伤后机体的损伤。
[Abstract]:Objective: through high-voltage electric heating effect, effect of electrochemical effect and electric field force on the body, vascular nerve, muscle damage to tissues and organs. High voltage electric burn injury pathological and physiological mechanisms are not completely understood. Studies suggest that oxidative stress is involved in the damage process of high-voltage electrical machine body. The results show that after burn the body produces a large number of reactive oxygen species (reactive oxygen, species, ROS), reactive oxygen species through lipid oxidation reaction of damaged tissue and organs, and the synergistic effect of vasoactive substances and factors, inflammation, oxidative stress injury caused by high voltage electric burn. Factors leading to systemic oxidative stress reaction of many, including plasma superoxide dismutase (superoxide dismutase SOD, malondialdehyde (malondialdehyde), and MDA) in the regulation of oxidative stress plays an important role in metal.SOD oxidase widely exists in organisms, can catalyze the superoxide anions By the disproportionation reaction of radicals, superoxide anion scavenging by cells and play a role of.MDA is the product of lipid peroxidation. At present, on serum SOD of rats after electric burns, MDA related research on quantitative and qualitative changes less, this study is to detect the high-voltage electric burn rat serum SOD, MDA content changes, and the effects of ulinastatin (Ulinastatin, UTI) to intervene, to explore after electrical burn serum SOD hypertension rats, the changing rules of MDA, prove the oxidative stress in the high voltage electric burn progressive damage occurrence and development process, explore the therapeutic effect of UTI on high voltage electric burn. Methods: 1 animal groups: experiment with 240 adult SD rats, rats were provided by the animal experimental center of Hebei Medical University (Certificate No. 832457), weight 291-362g, according to a randomized block design method will be divided into four groups randomly, 60 rats in each group: sham, high-voltage electric burn 缁，

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