阿维A与干扰素-α对小鼠淋巴瘤白血病模型的抑制作用

发布时间：2018-01-18 01:34

本文关键词：阿维A与干扰素-α对小鼠淋巴瘤白血病模型的抑制作用　出处：《吉林大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:探讨阿维A与干扰素-α联合应用对小鼠淋巴瘤白血病模型的抑制作用。方法:1.选用C57BL/6纯系雄性小鼠,6-8周龄,体重18-22g,鼠T淋巴瘤白血病细胞株EL-4悬浮培养于含20%马血清的DMEM培养液中常规培养。待细胞生长至对数期时,收集细胞,配置成1×107/ml的单细胞悬液。2.取0.1ml接种于C57BL/6小鼠右后肢靠背侧制作动物模型,于接种后第2日开始给药。3.实验分组给药:A组(空白对照组):PBS液0.05ml腹腔注射,PBS液0.1ml灌胃;B组(阿维A高剂量组):PBS液0.05ml腹腔注射,阿维A20mg/kg,0.1ml灌胃;C组(干扰素组):干扰素α-2β0.05ml腹腔注射,PBS液0.1ml灌胃;D组(联合用药组):干扰素α-2β0.05ml腹腔注射,阿维A20mg/kg,0.1ml灌胃;E组(阿维A低剂量组):PBS液0.05ml腹腔注射、阿维A10mg/kg,0.1ml灌胃。4.每日观察小鼠状态以及肿瘤生长情况,从第6天开始,测量肿瘤体积及小鼠重量。接种第14日处死小鼠,剥离肿瘤组织测定肿瘤体积及重量,测定肿瘤抑制率。组织固定、切片、HE染色。ELISA方法测定各组血清IL-6水平。荷瘤小鼠瘤重、肿瘤体积、IL-6细胞因子表达等计量资料用均数±标准差表示,应用SPSS 21.0软件对数据进行统计分析,结果以x±s表示,各组均值比较用LSD进行方差分析。P0.05为差异有显著性。结果:1.小鼠淋巴瘤白血病模型建立,成瘤率100%。2.接种后第7天,药物抑瘤作用最强,接种后第14天,药物抑瘤作用降低。阿维A能够抑制小鼠T细胞淋巴瘤白血病的增殖(p0.05)。干扰素-α可以抑制小鼠T细胞淋巴瘤白血病的增殖,(p0.05)且以干扰素与阿维A联合用药组抑瘤作用最明显,抑瘤率为51.38%。3.HE染色显示细胞异常分裂,未发现肿瘤转移。4.ELISA检测结果显示实验组较阴性对照组IL-6的浓度稍降低,但无显著性差异,各实验组之间浓度无差异。结论:1.在早期,阿维A和干扰素可以抑制小鼠T细胞淋巴瘤白血病的增殖,并以干扰素-α与阿维A组联合应用效果明显。2.干扰素-α、阿维A的抑瘤作用可能与下调IL-6有关,需要进一步实验来完善。3.在肿瘤快速生长期,单用阿维A和/或干扰素治疗淋巴瘤是不足够的,需要配合化疗或其他。
[Abstract]:Objective: to investigate the inhibitory effect of Avera combined with interferon-伪 (IFN- 伪) on murine lymphoma leukemia model. Methods: 1. C57BL / 6 male mice aged 6-8 weeks were selected. Body weight 18-22 g, murine T lymphoma leukemia cell line EL-4 suspension was cultured in DMEM medium containing 20% horse serum. When the cells grew to logarithmic phase, the cells were collected. A single cell suspension of 1 脳 10 7 / ml. 2. 0.1 ml was inoculated into the right hind limb of C57BL / 6 mice to make animal model. On the 2nd after inoculation, the drug was given to mice in group A (control group: 0. 05 ml, intraperitoneally injected with 0. 1 ml of PBS solution), and the control group was given intraperitoneal injection of 0. 1 ml of PBS solution (n = 0. 05 ml). Group B (Avera high dose group: 0. 05 ml) was injected intraperitoneally and Avera 20 mg / kg 路kg ~ (-1) / ml was intragastrically perfused. Group C (interferon group: interferon 伪 -2 尾 0.05ml intraperitoneally injected with 0.1 ml PBS solution; Group D (group D): interferon 伪 -2 尾 0.05ml was injected intraperitoneally and Avera 20mg / kg ~ (-1) was given intragastrically. Group E (Avera low dose group: 0. 05 ml) was injected intraperitoneally with Avera 10 mg / kg 路ml. 4. The status of mice and tumor growth were observed daily. From the 6th day, the tumor volume and the weight of the mice were measured. The mice were killed on 14th, the tumor volume and weight were determined by stripping the tumor tissue, the tumor inhibition rate, the tissue fixation and the section were determined. The levels of serum IL-6 in each group were determined by HE staining and Elisa. The measurement data of tumor weight and tumor volume IL-6 cytokine expression were expressed as mean 卤standard deviation. The data were analyzed by SPSS 21.0 software, and the results were expressed as x 卤s. Results: 1. Mouse lymphoma leukemia model was established, the tumorigenesis rate was 100. 2. 7 days after inoculation. The drug had the strongest inhibitory effect on tumor, and it was 14 days after inoculation. Avera can inhibit the proliferation of murine T-cell lymphoma leukemia. Interferon-伪 can inhibit the proliferation of mouse T-cell lymphoma leukemia. The inhibition rate was 51.38.3.HE staining showed abnormal cell division. No tumor metastasis. 4. Elisa results showed that the concentration of IL-6 in the experimental group was slightly lower than that in the negative control group, but there was no significant difference. Avera and interferon can inhibit the proliferation of murine T-cell lymphoma leukemia, and the combination of interferon- 伪 and avea group has obvious effect .2.Interferon- 伪. The antitumor effect of Avera may be related to down-regulation of IL-6, and further experiments are needed to perfect .3.In the rapid stage of tumor growth, it is not enough to treat lymphoma with avea and / or interferon alone. Need chemotherapy or other.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R733;R-332

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