肠道病毒EV71的双模检测及表面配体对CdSe量子点发光性质的影响

发布时间：2018-01-22 17:16

本文关键词： 量子点表面配体发光性质肠道病毒EV71 双模式免疫传感器　出处：《武汉大学》2017年硕士论文　论文类型：学位论文

【摘要】：纳米材料具有巨大的科研价值和广阔的应用前景。在众多纳米材料中,量子点和磁性纳米材料因其独特的性质而成为最具研究价值的纳米材料。众所周知,量子点的性质主要由其晶体结构、表面配体及所处环境决定。利用表面配体调控量子点发光性质具有灵活性多样性等优势。磁性纳米材料具有优良的磁性及易操控性,已被广泛用于生物医学分析化学检测中。基于此,本论文开展了以下工作:(1)将磁性纳米材料与酶放大策略、比色分析法、电化学免疫分析技术相结合,构建了基于双标记磁性纳米探针放大策略的比色和电化学双模式免疫传感器,并将其应用于现场检测肠道病毒EV71。整个实验可以在2 h内完成,比色分析和电化学检测的检出限分别达到1.0 ng/mL和10.0 pg/mL。该方法灵敏、简便,选择性好,抗干扰能力强,无需样品预处理和复杂昂贵的仪器及试剂,而且样品用量少至20μL。临床样品检测结果表明该方法的检测结果与RT-PCR结果相一致。(2)合成了以油胺/油酸为表面配体的硒化镉量子点(CdSe QDs),研究了辛硫醇取代中表面配体对其光致发光以及电致化学发光性质的影响。结果表明,辛硫醇能够强烈地猝灭CdSe QDs的带边发光;随着表面辛硫醇含量的增加,CdSe QDs的电致化学发光强度却逐渐增大,而电致化学发光光谱峰位置没有显著影响。据此提出了CdSe QDs的发光模型,揭示了表面配体对量子点发光机理的影响:CdSe QDs不仅存在与本征发射相关的激子态,还存在与表面密切相关的深能级陷阱态以及由内部杂质引起的浅能级陷阱态。
[Abstract]:Among many nanomaterials, quantum dots and magnetic nanomaterials have become the most valuable nanomaterials due to their unique properties. The properties of quantum dots are mainly due to their crystal structure. The surface ligands and their environment are determined. The use of surface ligands to regulate the luminescent properties of quantum dots has the advantages of flexibility and diversity. The magnetic nanomaterials have excellent magnetic properties and easy to control. Has been widely used in biomedical analytical chemistry detection. Based on this, this paper carried out the following work: 1) combining magnetic nanomaterials with enzyme amplification strategy, colorimetric analysis, electrochemical immunoassay. A colorimetric and electrochemical dual-mode immunosensor based on double-label magnetic nanoprobe amplification strategy was constructed and applied to the detection of enterovirus EV71.The whole experiment could be completed in 2 hours. The detection limits of colorimetric analysis and electrochemical detection were 1.0 ng/mL and 10.0 PG / mL, respectively. The method was sensitive, simple, selective and anti-jamming. There is no need for sample pretreatment and complex and expensive instruments and reagents. In addition, the dosage of the sample is as low as 20 渭 L. the results of clinical sample detection show that the results of this method are in agreement with the results of RT-PCR. 2) the cadmium selenide quantum dots with oleic acid / oleic acid as the surface ligands were synthesized. CdSe QDs. The effects of the ligands on the photoluminescence and electrochemiluminescence properties of CdSe QDs were studied. The results showed that octyl mercaptan could quench the band edge luminescence of CdSe QDs. The electrochemiluminescence intensity of CdSe QDs increases with the increase of octyl mercaptan content. However, the location of the electrochemiluminescence peak was not significantly affected. Based on this, the luminescence model of CdSe QDs was proposed. The effect of surface ligands on the luminescence mechanism of quantum dots is revealed. The exon states related to intrinsic emission are not only present in the QDs of: CdSe. There are also deep level trap states closely related to the surface and shallow level trap states caused by internal impurities.
【学位授予单位】：武汉大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R446.6;O657

【相似文献】