基于延胡索药材品质的全国适宜性区划研究

发布时间：2018-02-13 15:12

本文关键词： 延胡索延胡索甲素延胡素乙素指纹图谱生态适宜性适宜产区　出处：《浙江中医药大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:本课题通过对全国不同种植区的延胡索中延胡索甲素、延胡索乙素的含量测定,建立延胡索生物碱指纹图谱,为延胡索质量控制提供依据;通过分析延胡索中的延胡索甲素、延胡索乙素含量与生态因子的相关性,得到延胡索甲素、延胡索乙素各自的.空间分布估计,并对该药材进行全国适宜性区划,为种植优质延胡索、引种优质延胡索提供科学依据。方法:1.采用高相液相等度洗脱法测定5省15产区延胡索药材中延胡索甲素、延胡索乙素的含量。2.采用高效液相梯度洗脱法建立10产地延胡索生物碱指纹图谱及其共有模式图,运用SPSS软件分别采用相似度分析、聚类分析和主成分分析法全面评价延胡索质量。3.对15产区延胡索进行质量规划,运用R语言进行逐步回归法筛选相关因子,得出影响延胡索甲素、延胡索乙素的气候因子;运用AcrGIS系统并进行栅格计算,进行全国延胡索适宜性区划。结果:1.延胡索甲素与延胡索乙素两种成分的峰面积对数值与浓度对数值的线性关系良好(R≥0.997),平均加样回收率分别为107.52%和104.97%;15个产区延胡索样品中延胡索甲素和延胡索乙素的含量分别在0.08%-0.44%,0.03-0.20%区间。其中浙江缙云壶镇的延胡索甲素与延胡索乙素含量最高。2.建立了延胡索生物碱指纹图谱及共有模式。延胡索生物碱指纹图谱中有10个峰为10批次延胡索样品所共有,为其特征峰。不同产地延胡索之间指纹图谱相似度较高,与聚类分析和主成分分析结果一致。3.建立了延胡索全国适宜性区划。结论:1.对不同产地延胡索中延胡索甲素和延胡索乙素的含量进行测定,建立了延胡索药材质量控制体系,为提高延胡索质量提供依据。2.建立了延胡索的指纹图谱质量评价体系,生物碱含量相对较高的延胡索与其它延胡索相比相似度较低。3.延胡索种植基地最优区主要分布在最适宜产区包括河南省、河北省、云南省、四川省和广东省;适宜的产区包括浙江省、福建省、安徽省、广西壮族自治区、山东省、江苏省、贵州省和湖北省。
[Abstract]:Objective: to establish the fingerprint of Corydalis alkaloids in different planting areas of China by determining the contents of Corydalis and tetrahydropalmatine in order to provide the basis for the quality control of Corydalis corydalis. Based on the analysis of the correlation between the contents of Corydalis and tetrahydropalmatine and ecological factors, the spatial distribution of Corydalis and tetrahydropalmatine were estimated, and the national suitability regionalization was carried out. To provide scientific basis for planting and introducing high quality Corydalis corydalis. Methods: 1. High phase liquid phase isometric elution method was used for the determination of Corydalis in Corydalis officinalis in 15 producing regions of 5 provinces. The content of tetrahydropalmatine. 2. HPLC gradient elution method was used to establish the alkaloid fingerprint and common pattern map of Corydalis from 10 producing areas, and the similarity analysis was used by SPSS software, respectively. Cluster analysis and principal component analysis (PCA) were used to evaluate the quality of Corydalis corydalis. The quality planning of Corydalis corydalis was carried out in 15 production areas, and the relative factors were screened by using R language step by step regression method, and the climatic factors affecting Corydalis and tetrahydropalmatine were obtained. Using AcrGIS system and grid calculation, Results: 1. The linear relationship between the peak area logarithm and concentration logarithm of Corydalis A and tetrahydropalmatine was good. The average recoveries were 107.52% and 104.97, respectively, in 15 producing regions. The contents of Corydalis A and tetrahydropalmatine in the samples were in the range of 0.08-0.44, 0.03-0.20%, respectively. The contents of Corydalis A and tetrahydropalmatine were the highest in Huzhen, Jinyun, Zhejiang Province. The alkaloid fingerprint and common pattern of Corydalis were established. 10 peaks of alkaloid fingerprint of Corydalis corydalis were found in 10 batches of Corydalis corydalis samples. The fingerprint of Corydalis corydalis from different areas was similar. The results of cluster analysis and principal component analysis were in agreement with the results of cluster analysis and principal component analysis. The national suitability regionalization of Corydalis was established. Conclusion: 1. The content of Corydalis and tetrahydropalmatine in Rhizoma Corydalis from different habitats was determined, and the quality control system of Corydalis was established. In order to improve the quality of Rhizoma Corydalis, the fingerprint quality evaluation system of Corydalis corydalis was established. The relative high alkaloid content of Corydalis corydalis compared with other Corydalis corydalis. 3. The optimum area of Yanhusuo planting base mainly distributed in the most suitable production areas including Henan Province, Hebei Province, Yunnan Province, Sichuan Province and Guangdong Province; Suitable production areas include Zhejiang Province, Fujian Province, Anhui Province, Guangxi Zhuang Autonomous region, Shandong Province, Jiangsu Province, Guizhou Province and Hubei Province.
【学位授予单位】：浙江中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S567.239;R284.1

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